Mark Edward Powers

US Patent:

20070141105, Jun 21, 2007

Filed:

Oct 6, 2006

Appl. No.:

11/539580

Inventors:

Thomas Stein - Myersville MD, US
Mark Powers - New Market MD, US
Jonathan Cooper - Walkersville MD, US
Sorin Damian - Frederick MD, US
Pieter Johannes Huiberts - Frederick MD, US
Kenneth Guiseley - Hope ME, US

International Classification:

A61F 2/02
A61K 9/14
C12N 5/02

US Classification:

424423000, 424486000, 435404000

Abstract:

Biocompatible matrices or implants on which one or more specific cell-interactive molecules (“biomolecules”) can be immobilized have been developed. The matrices allow for the independent control of ligand concentration and matrix strength. In one embodiment, the matrix or implant is modified with one or more moieties capable of complexing bioconjugates prepared from one or more biomolecules. Suitable moieties include phenyl boronic acid complexing agents, such as salicylhydroxamic acid, which can complex to one or more biomolecules containing one or more phenyl boronic acid moieties. The biomolecules may be anchored to the matrix via a spacer molecule, which may allow for greater mobility of the biomolecules in aqueous solution. In one embodiment, the matrix is a hydrogel material which has been doubly-derivatized, wherein ligand concentration and matrix strength can be independently controlled. The matrices and implants can be used in vivo and in vitro applications including diagnostics, biosensors, bioprocess engineering, tissue engineering, regeneration and repair, and drug delivery.

US Patent:

20130130374, May 23, 2013

Filed:

Aug 3, 2011

Appl. No.:

13/813913

Inventors:

Mark Powers - New Market MD, US
Rafal Witek - Burlington NC, US
Kirsten Amaral - Cary NC, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12N 5/071

US Classification:

435350, 435325, 435351, 435352, 435370, 4353052

Abstract:

Methods for preparing a variety of cell (e.g., hepatocyte) preparations and preparations so prepared are described. Uses of such preparations are described. In one embodiment, centrifugal elutriation is used to separate hepatocytes with preferred characteristics. Such hepatocyte preparations may be used for cryopreservation, multiple cryopreservations, in vitro assays, plating and other methods for which preparations of hepatocytes are useful.