Mark E Quesada

US Patent:

54361303, Jul 25, 1995

Filed:

Oct 26, 1993

Appl. No.:

8/143377

Inventors:

Richard A. Mathies - Contra Costa County CA
Xiaohua C. Huang - Mt. View CA
Mark A. Quesada - San Francisco CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12Q 168
C12P 1934
G01N 3350

US Classification:

435 6

Abstract:

A DNA sequencing method described which uses single lane or channel electrophoresis. Sequencing fragments are separated in said lane and detected using a laser-excited, confocal fluorescence scanner. Each set of DNA sequencing fragments is separated in the same lane and then distinguished using a binary coding scheme employing only two different fluorescent labels. Also described is a method of using radio-isotope labels.