Kevin S. Bodner - San Mateo CA Alfred P. Madden - Colorado Springs CO Joseph Jackson - Half Moon Bay CA Jason H. Halsey - San Francisco CA Mark T. Reed - Menlo Park CA Ward Frye - Foster City CA Mark F. Oldham - Los Gatos CA Stephen E. Moring - Lawrence KS Jon Hoshizaki - Cupertino CA
Assignee:
PE Corporation (NY) - Foster City CA
International Classification:
B01D 6100
US Classification:
210650, 21032175, 210474, 210261, 422101, 422104
Abstract:
The present invention provides multi-well plates and column arrays in which samples (e. g. , cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering.
Kevin S. Bodner - San Mateo CA Alfred P. Madden - Colorado Springs CO Joseph Jackson - El Granada CA Jason H. Halsey - San Francisco CA Mark T. Reed - Menlo Park CA Ward Frye - Foster City CA Mark F. Oldham - Los Gatos CA Stephen E. Moring - Lawrence KS Jon Hoshizaki - Cupertino CA
Assignee:
Applera Corporation - Foster City CA
International Classification:
B01L 300
US Classification:
422 99, 4352831, 436174, 215200
Abstract:
The present invention provides multi-well plates and column arrays in which samples (e. g. , cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering.
Manually-Operable Multi-Well Microfiltration Apparatus And Method
A multi-well microfiltration apparatus and method are provided and feature a manual touch-off system for transferring pendent drops hanging from discharge-conduits of a discharge-conduit array to respective receiving wells or receiving holes of a corresponding receiving array, with minimum or no cross-contamination between the discharge conduits, or the receiving wells or receiving holes. The manual touch-off is achieved by manually shifting a carriage that supports one of the arrays, into a position whereat pendent drops of fluid hanging from the distal ends of the discharge conduits contact the inner sidewalls of the corresponding receiving wells or receiving holes of the receiving array.
Douwe D. Haga - Redwood City CA, US Michael R. Flatbush - San Ramon CA, US Michael Mosseau - Santa Clara CA, US Tony S. Yan - Millbrae CA, US Willy Wiyatno - Union City CA, US Mark T. Reed - Menlo Park CA, US
Assignee:
Applera Corporation - Foster City CA
International Classification:
G01N 21/49 G01N 21/85 G01N 15/06
US Classification:
250577, 356624, 73293
Abstract:
The present application relates to an apparatus and method for measuring liquid levels in small-volume wells.
Mark T. Reed - Menlo Park CA, US Albert L. Carrillo - Redwood City CA, US Ian A. Harding - San Mateo CA, US
Assignee:
Applera Corporation - Foster City CA
International Classification:
B01L 3/00
US Classification:
422102, 422100, 422104
Abstract:
A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an assay input layer having a first surface and an opposing second surface. The assay input layer can comprise an assay input port extending from the first surface to the second surface, a venting manifold formed in the second surface, and a vent hole in fluid communication with the venting manifold and atmosphere. An output layer can comprise a plurality of staging capillaries each having an inlet and an outlet. A venting layer can be disposed between the assay input layer and the output layer. The venting layer can comprise a plurality of microfluidic channels, a flow aperture being in fluid communication with the assay input port and the plurality of microfluidic channels, and a plurality of vent apertures each in fluid communication with at least one of the plurality of staging capillaries. Two or more of the plurality of vent apertures can be in fluid communication with the venting manifold.
Encoding And Decoding Reactions For Determining Target Polynucleotides
Kai Qin Lao - Pleasanton CA, US Mark T. Reed - Menlo Park CA, US
Assignee:
Applied Biosystems, LLC - Carlsbad CA
International Classification:
C12Q 1/68 C07H 21/04
US Classification:
435 6, 536 2431, 536 2432, 536 2433
Abstract:
The present invention is directed to methods, reagents, and kits for detecting the presence or absence of (or quantifying) target polynucleotide sequences in at least one sample using encoding and decoding reactions. When a particular target polynucleotide is present in a sample, a reaction product is formed in the encoding reaction that includes addressable primer portions. At least one label and at least one address primer is employed in the decoding amplification reaction thereby providing a detectable signal value depending upon whether a sequence is present or absent. In some embodiments universal bases and reduced libraries of probes can be employed for highly multiplexed analysis of target polynucleotides.
Mark T. Reed - Menlo Park CA, US Albert L. Carrillo - Redwood City CA, US Ian A. Harding - San Mateo CA, US
Assignee:
Applied Biosystems, LLC - Carlsbad CA
International Classification:
B01L 11/00
US Classification:
422103, 422100, 422102
Abstract:
A filling apparatus for filling a microplate. The microplate can comprise a plurality of wells each sized to receive an assay. A substrate can comprise a first surface and an opposing second surface, a first assay input port for receiving the assay disposed on the first surface, a plurality of staging capillaries extending through the substrate, and a first plurality of microfluidic channels fluidly coupling the first assay input port with at least one of the plurality of staging capillaries. Each of the plurality of staging capillaries can comprise an inlet and an outlet and be sized to receive the assay.
Medicine Doctors
Mark Reed, Placentia CA - DPM (Doctor of Podiatric Medicine)
American Board of Podiatric Surgery re-certification, 2006
Languages:
English
Hospitals:
Mark and Melanie Reed, DPM 1275 N Rose Dr Suite 136, Placentia, CA 92870
Mark and Melanie Reed, DPM 21580 Yorba Linda Blvd, Yorba Linda, CA 92887
Placentia - Linda Hospital 1301 Rose Drive, Placentia, CA 92870
Philosophy:
As a Podiatrist, Dr. Reed treats each patient as a partner in their medical treatment to improve the patient's outcome with an emphasis on minimizing pain and optimizing results. Dr. Reed has 25 years of experience as a Podiatric surgeon and trained at the top residency in the nation at UCSF / VASF in San Francisco. Dr. Reed is ABPS Board Certified and is a Fellow in Wound Care for APWCA. Dr. Reed trained as a medical student at USC / LA County Medical Center.
Education:
Medical School Usc / La County Medical Center-California College Of Podiatric Medicine Graduated: 1989 Medical School University Of California San Francisco / Veterans Administration Medical Center Graduated: 1990 Medical School California State University, Los Angeles Graduated: 1982 Medical School Hadassah University Hospital Podiatric Surgical Fellowship, Jerusalem, Isreal Graduated: 1988
Dr. Reed graduated from the University of Tennessee College of Medicine at Memphis in 1976. He works in Memphis, TN and specializes in Family Medicine. Dr. Reed is affiliated with Methodist North Hospital.
West Clinic PCWest Cancer Center 7945 Wolf Riv Blvd, Germantown, TN 38138 9016830055 (phone), 9016859718 (fax)
West Clinic PCWest Clinic 2001 State Dr, Corinth, MS 38834 6622863694 (phone), 6622863853 (fax)
Education:
Medical School University of Tennessee College of Medicine at Memphis Graduated: 1987
Procedures:
Chemotherapy
Conditions:
Abnormal Vaginal Bleeding Breast Disorders Conditions of Pregnancy and Delivery Female Infertility Hypertension (HTN)
Languages:
English
Description:
Dr. Reed graduated from the University of Tennessee College of Medicine at Memphis in 1987. He works in Corinth, MS and 1 other location and specializes in Gynecologic Oncology. Dr. Reed is affiliated with Baptist Memorial Hospital-Collierville, Methodist Hospital South, Methodist Rehabilitation Center and Saint Francis Hospital Memphis.
Valley Orthopedic Associates Surgery CenterOrthopedic Specialists Of Seattle 5350 Tallman Ave NW STE 500, Seattle, WA 98107 2067848833 (phone), 2067840676 (fax)
Valley Orthopedic Associates Surgery CenterSeattle Orthopedic Center 2409 N 45 St, Seattle, WA 98103 2066338100 (phone), 2066336107 (fax)
Education:
Medical School University of Alabama School of Medicine Graduated: 2006
Procedures:
Arthrocentesis Hallux Valgus Repair Hip/Femur Fractures and Dislocations Joint Arthroscopy Lower Leg/Ankle Fractures and Dislocations Occupational Therapy Evaluation Knee Arthroscopy Lower Arm/Elbow/Wrist Fractures and Dislocations Shoulder Surgery
Conditions:
Fractures, Dislocations, Derangement, and Sprains
Languages:
English Spanish
Description:
Dr. Reed graduated from the University of Alabama School of Medicine in 2006. He works in Seattle, WA and 1 other location and specializes in Orthopaedic Surgery. Dr. Reed is affiliated with Northwest Hospital & Medical Center, Swedish Medical Center - First Hill, Swedish Medical Center Ballard and Swedish Medical Center Cherry Hill.
Joseph J Schifini MD 600 S Tonopah Dr STE 240, Las Vegas, NV 89106 7028700011 (phone), 7028701144 (fax)
Virginia Mason Medical Center Podiatry 1100 9 Ave, Seattle, WA 98101 2062237527 (phone), 8888622737 (fax)
Procedures:
Hallux Valgus Repair
Conditions:
Hallux Valgus Plantar Fascitis Tinea Pedis
Languages:
English Spanish
Description:
Dr. Reed works in Seattle, WA and 1 other location and specializes in Podiatric Medicine. Dr. Reed is affiliated with Valley Hospital Medical Center and Virginia Mason Medical Center.
Mark O Reed MD 600 S Tonopah Dr STE 240, Las Vegas, NV 89106 7023833170 (phone), 7023823736 (fax)
Education:
Medical School St. George's University School of Medicine, St. George's, Greneda Graduated: 1983
Languages:
English Spanish
Description:
Dr. Mark O. Reed graduated from the St. George's University School of Medicine, St. George's, Grenada in 1983. Residency training completed at University of Missouri. He works in Las Vegas, NV with a private practice and specializes in Physical Medicine & Rehabilitation. Dr. Reed treats musculoskeletal conditions, post operative orthopedic, post operative and non surgical spine patients,
2011 to 2000 Vice President Division Sales Manager On PremiseEstate Fine Wine San Francisco, CA 2009 to 2011 Branch Manager - Director of SalesVice President Division Sales Manager On Premise 2007 to 2009Named
2001 to 2006 Wine & Spirits Sales RepresentativeAT&T San Francisco, CA 1999 to 2001 Beverage and In-Seat Service ManagerBeverage
1996 to 1999 Beverage and Catering Manager, Candlestick Park & Cow PalaceHyatt Corporation
1995 to 1996 Banquet and Convention ManagerAll Seasons Bed and Breakfast Restaurant Prescott, AZ 1993 to 1995 Owner/ChefManor Inn and Restaurant Ukiah, CA 1992 to 1993 General ManagerVirginia Museum of Fine Arts Richmond, VA 1985 to 1991 Restaurant and Event ManagerHost Marriott Corporation Dulles Airport Richmond, VA 1984 to 1985 Outlets Manager
Oct 1989 to Nov 2010 Senior BuyerEastern Electronics
Dec 1983 to Sep 1989 Purchasing AgentColeco Industries
Jun 1983 to Dec 1983 BuyerEastern Electronics
Jan 1980 to Jun 1983 BuyerTimes Defense Products
Jun 1979 to Jan 1980 Expediter/Stock Room Clerk
Education:
University of San Francisco San Francisco, CA Jan 2011 to Jan 2011 MSSSCM in Sustainable Supply Chain ManagementState University of New York at Canton Jan 1978 to Jan 1978 HVACState University of New York at Canton Jan 1977 to Jan 1977 Electrical TechGeneral Brown High School Jan 1972 to Jan 1976 Regents Diploma with Honors
Skills:
purchasing, quality auditing, global sourcing, Lean White Belt
Isbn (Books And Publications)
Produced Water 2 : Environmental Issues and Mitigation Technologies
Clarkesville, GeorgiaDeveloper Representative/Realtor at Land Specialti... Past: Substitute Teacher at Kelly Educational Staffing, Mayor at City of Baldwin Georgia...
at calls centersplus a pledge by Verizon to hire even more U.S. workersas a key piece of the new contract. Were especially proud of our commitment to 1,400 new hireshigh quality and well-paying American jobs, Mark Reed, Verizons chief administrative officer, crowed at the time.
Date: Oct 13, 2016
Category: Business
Source: Google
Star Wars fans dress up, line up and wait for premiere
"I was born in 1977 so I just grew up with it. One of my first memories is seeing Star Wars at home on VHS when I was 3 and I was angry because I couldn't watch it again immediately. It formed who we are for a lot of our generations," said Star Wars fan Mark Reed.
Date: Dec 17, 2015
Category: Entertainment
Source: Google
Schnepf: Ponder shows signs of hope in loss to Chicago
oorhead placed its hope on a transfer quarterback from the University of Minnesota. The anticipated savior was a senior named Marc Trestman. He ended up starting only five games, completing 34 of 91 passes for 423 yards. He was eventually replaced by Mark Reed, who would later become an All-American.
"The caretaker just didn't know, and she went outside and picked these mushrooms and made dinner, and she ate some of it herself," said Lt. Mark Reed of the Placer County sheriff's office. "It's definitely a sad, sad thing."
The poisonings are believed to be accidental, and the caregiver who allegedly prepared the soup is one of the people hospitalized, Sheriff's Lt. Mark Reed told the Associated Press. The three others hospitalized were elderly residents of the Gold Age Villa.
The caregiver who prepared the soup was among the six people sickened, said Sheriff's Lt. Mark Reed. The conditions of the four people hospitalized were not immediately known, and their names have not been released.
Date: Nov 11, 2012
Category: Health
Source: Google
2 elderly women die, 4 people sickened after eating wild mushrooms
Placer County sheriff's Lt. Mark Reed said the incident, reported Friday morning at the Gold Age Villa in Loomis, was believed to be an accident, the Bee reported. Reed said the dead were identified asBarbara Lopes, 86, and Teresa Olesniewicz, 73. The caregiver was among the four who were hospitali
Sheriff's Lt. Mark Reed says the caregiver who prepared the soup was among the six people sickened. The conditions of the four people hospitalized were not immediately known, and their names have not been released.
Santa Clara, Ca Milwaukee Wisc San Jose Mountain View Sunnyvale Milwaukee Bloomfleld
Work:
SanMax Solutions Drivedeals.com Inclose Designs NCA ASI Malabs
Education:
Cupertino High School, De Anza College, San Jose State
Mark Reed
Work:
Reading Borough Council - Senior Social Worker
Education:
University of Reading - Higher Specialist SW, Powers Hall Primary School - Primary School, King Edward VI Grammar School, Chelmsford - Secondary School, Bulmershe College of HE - B. Ed, Brunel University College - Dip. SW
Mark Reed
Work:
Buckeye Nissan - Parts Delivery Columbus Zoo (2005-2010)
Education:
Bowling Green State University - Accounting
Mark Reed
Work:
Self - Site builder (2000)
Education:
College of Hard Knocks - Life
Tagline:
Dr. CSS
Mark Reed
Work:
USAF
Education:
American Military University - MBA
Mark Reed
Work:
GSTC - OTR DRIVER
Education:
INTERNATIONAL SCHOOL OF DETOXIFICATION
Mark Reed
Education:
FLORIDA CAREED COLLEGE - IT
Bragging Rights:
PINE BLUFF HIGH SCHOOL
Mark Reed
Work:
United Country Mid West eServices Inc. - Data Entry (8)