Mark T Reynolds

US Patent:

8399222, Mar 19, 2013

Filed:

Nov 25, 2009

Appl. No.:

12/625609

Inventors:

Amara Siva - Oceanside CA, US
Mark Alan Reynolds - Carlsbad CA, US

Assignee:

Gen-Probe Incorporated - San Diego CA

International Classification:

C12P 19/34
C12Q 1/68

US Classification:

435 912, 435 612

Abstract:

Compositions and methods are provided for the detection of small RNA target nucleic acids, preferably miRNA target nucleic acids, wherein the compositions and methods provide for sensitive and specific detection of the target nucleic acids. The compositions and methods include using one or more of a first amplification oligomer that is preferably an extender primer, a target capture oligomer that is preferably at least partially double stranded, a promoter primer/provider, a reverse primer that is preferably a universal primer and a detection probe. The compositions and methods are useful for diagnostics, prognostics, monitoring the effectiveness of treatment and/or determining a treatment.