Marlene M Darfler

US Patent:

7473532, Jan 6, 2009

Filed:

Mar 10, 2004

Appl. No.:

10/796288

Inventors:

Marlene M. Darfler - Derwood MD, US
David B. Krizman - Gaithersburg MD, US

Assignee:

Expression Pathology, Inc. - Gaithersburg MD

International Classification:

G01N 33/53
G01N 1/00
G01N 35/00
G01N 37/00
C07K 1/12
C07K 1/16
C07K 1/36
C07K 2/00

US Classification:

435 72, 435 405, 435378, 530300, 530344, 530345, 530412, 530417

Abstract:

The current invention provides a method for directly converting histopathologically processed biological samples, tissues, and cells into a multi-use biomolecule lysate. This method allows for simultaneous extraction, isolation, solublization, and storage of all biomolecules contained within the histopathologically processed biological sample, thereby forming a representative library of said sample. This multi-use biomolecule lysate is dilutable, soluble, capable of being fractionated, and used in any number of subsequent experiments.

US Patent:

7906301, Mar 15, 2011

Filed:

May 25, 2006

Appl. No.:

11/915582

Inventors:

David B. Krizman - Gaithersburg MD, US
Marlene M. Darfler - Derwood MD, US

Assignee:

Expression Pathology, Inc. - Rockville MD

International Classification:

G01N 33/53

US Classification:

435 41, 435 71, 435 72

Abstract:

The invention provides methods for multiplex analysis of biological samples of formalin-fixed tissue samples. The invention provides for a method to achieve a multiplexed, multi-staged plurality of Liquid Tissue preparations simultaneously from a single histopathologically processed biological sample, where the protocol for each Liquid Tissue preparation imparts a distinctive set of biochemical effects on biomolecules procured from histopathologically processed biological samples and which when each of the preparations is analyzed can render additive and complementary data about the same histopathologically processed biological sample.

US Patent:

8293485, Oct 23, 2012

Filed:

Mar 15, 2011

Appl. No.:

13/048522

Inventors:

David B. Krizman - Gaithersburg MD, US
Marlene M. Darfler - Derwood MD, US

Assignee:

Expression Pathology, Inc. - Gaithersburg MD

International Classification:

G01N 33/53

US Classification:

435 71, 436518

Abstract:

The invention provides methods for multiplex analysis of biological samples of formalin-fixed tissue samples. The invention provides for a method to achieve a multiplexed, multi-staged plurality of Liquid Tissue preparations simultaneously from a single histopathologically processed biological sample, where the protocol for each Liquid Tissue preparation imparts a distinctive set of biochemical effects on biomolecules procured from histopathologically processed biological samples and which when each of the preparations is analyzed can render additive and complementary data about the same histopathologically processed biological sample.

US Patent:

8394609, Mar 12, 2013

Filed:

Dec 1, 2009

Appl. No.:

12/629045

Inventors:

Irina Nazarenko - Gaithersburg MD, US
Ayoub Rashtchian - Gaithersburg MD, US
Joseph Solus - Gaithersburg MD, US
Richard Pires - Mount Airy MD, US
Marlene Darfler - Derwood MD, US
Gulilat Gebeyehu - Potomac MD, US
Mekbib Astatke - Germantown MD, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12P 19/34

US Classification:

435 912

Abstract:

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

US Patent:

8609345, Dec 17, 2013

Filed:

May 25, 2006

Appl. No.:

11/915581

Inventors:

David B. Krisman - Gaithersburg MD, US
Marlene M. Darfler - Derwood MD, US
Thomas G. Guiel - Gaithersburg MD, US
Casimir P. Eitner - Washington DC, US

Assignee:

Expression Pathology Incorporated - Los Angeles CA

International Classification:

G01N 33/53
G01N 33/574

US Classification:

435 71, 435 614, 435 405, 435381

Abstract:

The invention provides methods for diagnosing diseases such as cancer and other conditions using biological samples. Liquid Tissue samples prepared from histopathologically prepared tissue obtained from a subject surprisingly can be used to identify and, optionally, to quantify analytes that are diagnostic of the presence of a disease, condition or syndrome in the subject.

US Patent:

20030165859, Sep 4, 2003

Filed:

Dec 27, 2001

Appl. No.:

10/026952

Inventors:

Irina Nazarenko - Gaithersburg MD, US
Ayoub Rashtchian - Gaithersburg MD, US
Joseph Solus - Madison AL, US
Richard Pires - Germantown MD, US
Marlene Darfler - Derwood MD, US
Gulilat Gebeyehu - Potomac MD, US
Mekbib Astatke - Germantown MD, US

Assignee:

Invitrogen Corporation

International Classification:

C12Q001/68
C07H021/04
C12P019/34

US Classification:

435/006000, 435/091200, 536/024300

Abstract:

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

US Patent:

20040247777, Dec 9, 2004

Filed:

Jun 4, 2004

Appl. No.:

10/863833

Inventors:

Bradley Ringeisen - Alexandria VA, US
Jason Barron - Alexandria VA, US
Peter Wu - Ashland OR, US
David Krizman - Gaithersburg MD, US
Marlene Darfler - Derwood MD, US

International Classification:

B05D003/00

US Classification:

427/002100, 427/596000

Abstract:

A method of laser forward transfer is disclosed. Photo energy is directed through a photon-transparent support and absorbed by an interlayer coated thereon. The energized interlayer causes the transfer of specific regions of a heterogeneous tissue sample coated thereon across a gap and onto a receiving substrate or into a receiving vessel.

US Patent:

20050042666, Feb 24, 2005

Filed:

Sep 2, 2004

Appl. No.:

10/932045

Inventors:

Irina Nazarenko - Gaithersburg MD, US
Ayoub Rashtchian - Gaithersburg MD, US
Joseph Solus - Madison AL, US
Richard Pires - Damascus MD, US
Marlene Darfler - Derwood MD, US
Gulilat Gebeyehu - Potomac MD, US
Mekbib Astatke - Germantown MD, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435006000, 435091200

Abstract:

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.