Mary Therese Fletcher

US Patent:

44606940, Jul 17, 1984

Filed:

Mar 9, 1982

Appl. No.:

6/356348

Inventors:

Mary A. Fletcher - Miami FL

Assignee:

University of Miami - Coral Gables FL

International Classification:

G01N 3354
G01N 3356
C07G 700

US Classification:

436531

Abstract:

A novel purification scheme is described for obtaining two novel and useful forms of bovine glycoprotein (BGP) from bovine erythrocytes, each of which acts as an antigen in testing for the presence of the heterophile antibodies of human infectious mononucleosis. The first, or partially purified, BGP is obtained from crude BGP and contains about 10% by weight of a complex glycoplipid. It forms a single band upon gel electrophoresis at pH 7. 0 under specified conditions. The second, or homogeneous, BGP is obtained by removing essentially all of the complex glycolipid. It forms substantially a single band upon gel electrophoresis at pH 7. 0 under specified conditions. Both forms may be used to detect or quantify hemagglutination inhibition of a test sample (and hence to determine the presence or extent to mononucleosis infection) in a glass slide test wherein the partially purified or homogeneous BGP is carried on latex or synthetic resin beads. The homogeneous BGP is also useful in an immunoassay, preferably a radioimmunoassay, for heterophile antibodies characteristic of infectious mononucleosis.

US Patent:

45254591, Jun 25, 1985

Filed:

Jan 27, 1982

Appl. No.:

6/343235

Inventors:

Mary A. Fletcher - Miami FL

Assignee:

University of Miami - Coral Gables FL

International Classification:

G01N 3350
B01J 3906
B01J 4106
B01D 902

US Classification:

436544

Abstract:

Preparations useful in the diagnosis of infectious mononucleosis are disclosed. Horse and sheep erythrocytes are purified and essentially homogenous glycoproteins are extracted. The purified glycoproteins are useful as antigens in testing for the presence of the heterophile antibodies of infectious mononucleosis and in the preparation of reagents for the enumeration of rosetting lymphocytes without having to have available fresh sheep blood.