Mary F Longo

US Patent:

6703484, Mar 9, 2004

Filed:

Jan 8, 1998

Appl. No.:

09/004068

Inventors:

Deb Chatterjee - North Potomac MD
Mary Longo - Germantown MD
Elizabeth Flynn - Columbia MD
Robert Oberfelder - Woodland TX

Assignee:

Invitrogen Corporation - DE

International Classification:

C07K 100

US Classification:

530350, 435 691, 435 681, 435 697

Abstract:

The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as thioredoxin or a modified thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

US Patent:

7223566, May 29, 2007

Filed:

Dec 8, 2003

Appl. No.:

10/728766

Inventors:

Deb K Chatterjee - North Potomac MD, US
Mary Longo - Germantown MD, US
Elizabeth Flynn - Columbia MD, US
Robert Oberfelder - Woodland TX, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12P 21/04
C12P 21/06
C07K 1/00

US Classification:

435 697, 530350, 435 691

Abstract:

The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as thioredoxin or a modified thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

US Patent:

7259242, Aug 21, 2007

Filed:

Jan 11, 2006

Appl. No.:

11/329401

Inventors:

Deb K. Chatterjee - North Potomac MD, US
Mary C. Longo - Germantown MD, US
Elizabeth Flynn - Columbia MD, US
Robert W. Oberfelder - Woodland TX, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C07K 1/00
C12P 21/06
C12P 21/04

US Classification:

530350, 435 691, 435 681, 435 697

Abstract:

The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as thioredoxin or a modified thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

US Patent:

7265206, Sep 4, 2007

Filed:

Jan 11, 2006

Appl. No.:

11/329418

Inventors:

Deb K. Chatterjee - North Potomac MD, US
Mary C. Longo - Germantown MD, US
Elizabeth Flynn - Columbia MD, US
Robert W. Oberfelder - Woodland TX, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C07K 1/00
C12P 21/06
C12P 21/04

US Classification:

530350, 435 691, 435 681

Abstract:

The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as thioredoxin or a modified thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

US Patent:

8012715, Sep 6, 2011

Filed:

Dec 29, 2006

Appl. No.:

11/618723

Inventors:

Deb K. Chatterjee - North Potomac MD, US
Mary Longo - Germantown MD, US
Elizabeth Flynn - Columbia MD, US
Robert Oberfelder - Woodland TX, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C07K 1/00
C12P 21/04

US Classification:

435 697, 530350

Abstract:

The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as thioredoxin or a modified thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

US Patent:

8389681, Mar 5, 2013

Filed:

Dec 29, 2006

Appl. No.:

11/618725

Inventors:

Deb K. Chatterjee - North Potomac MD, US
Mary Longo - Germantown MD, US
Elizabeth Flynn - Columbia MD, US
Robert Oberfelder - Woodland TX, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C07K 1/00

US Classification:

530350

Abstract:

The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as thioredoxin or a modified thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

US Patent:

8519099, Aug 27, 2013

Filed:

Jan 13, 2011

Appl. No.:

13/006354

Inventors:

Deb Chatterjee - North Potomac MD, US
Mary Longo - Germantown MD, US
Elizabeth Flynn - Columbia MD, US
Robert Oberfelder - Woodland TX, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C07K 1/00

US Classification:

530350

Abstract:

The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as thioredoxin or a modified thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

US Patent:

20020168706, Nov 14, 2002

Filed:

Mar 7, 2002

Appl. No.:

10/091538

Inventors:

Deb Chatterjee - N. Potomac MD, US
Mary Longo - Germantown MD, US

Assignee:

Invitrogen Corporation

International Classification:

C12P021/06
C12P019/34

US Classification:

435/068100, 435/091200

Abstract:

Compositions, systems, kits and methods relating to in vitro synthesis are provided. The system includes one or more extracts having reduced activity of an enzyme that catalyses hydrolysis of high energy phosphate bonds or hydrolysis or formation of phosphodiester bonds, an inhibitor that inhibits hydrolysis of high energy phosphate bonds or hydrolysis or formation of phosphodiester bonds, and/or at least two energy sources. The composition may include a nucleic acid template and one or more products of the nucleic acid template.