Mary Frances Lopez

US Patent:

7906630, Mar 15, 2011

Filed:

Apr 26, 2005

Appl. No.:

11/114629

Inventors:

Alvydas Mikulskis - West Roxbury MA, US
Mary F. Lopez - Bedford MA, US

Assignee:

PerkinElmer Heath Sciences, Inc. - Waltham MA

International Classification:

A23J 1/00
A61K 38/00
A61K 51/00

US Classification:

530416, 514 11, 424 169

Abstract:

The invention provides a method for isolating a cargo peptide from a sample containing a cargo peptide-carrier protein complex. The method involves contacting a sample comprising a cargo peptide-carrier protein complex with a binding moiety selective for the carrier protein, under conditions wherein the carrier protein binds non-covalently to the binding moiety, and wherein the binding moiety is attached to a support, dissociating the cargo peptide from the cargo peptide-carrier protein complex, wherein the carrier protein remains bound to the binding moiety, and collecting the cargo peptide, whereby the cargo peptide is isolated from the sample.

US Patent:

8288110, Oct 16, 2012

Filed:

Dec 4, 2007

Appl. No.:

11/950416

Inventors:

Mary F. Lopez - Bedford MA, US
Scott Kuzdzal - Shelton CT, US

Assignee:

PerkinElmer Health Sciences, Inc. - Waltham MA

International Classification:

G01N 33/53

US Classification:

435 71

Abstract:

The disclosure features, inter alia, a variety of biomarkers (e. g. , biomarker peptides), compositions, and kits for use in methods for diagnosing a cancer. The biomarkers can be used, e. g. , in methods for detecting a cancer in a subject. In addition, the disclosure also features methods of selecting a therapy for, and/or administering a therapy (e. g. , a therapy comprising an anti-cancer agent) to, a subject having a cancer.

US Patent:

8383417, Feb 26, 2013

Filed:

Dec 22, 2009

Appl. No.:

12/644284

Inventors:

Mary Frances Lopez - Boston MA, US
David A. Sarracino - Andover MA, US
Amol Prakash - Cambridge MA, US
Randall W. Nelson - Phoenix AZ, US
Paul Oran - Scottsdale AZ, US
Bryan E. Krastins - Somerville MA, US
Taha Rezai - Cambridge MA, US

Assignee:

Thermo Finnigan, LLC - San Jose CA
Intrinsic Bioprobes, Inc. - Tempe AZ

International Classification:

G01N 24/00
G01N 33/00

US Classification:

436173, 436 86, 436 89

Abstract:

Methods are described for monitoring the amounts of PTH variants in a biological sample by digesting the sample to produce surrogate peptides specific to the targeted PTH variants, and detecting and quantifying the surrogate peptides by selective reaction monitoring (SRM) mass spectrometry, using a set of precursor-to-product ion transitions optimized for sensitivity and selectivity. The PTH variants, or a portion thereof, may be concentrated in the sample by means of immunoaffinity capture or other suitable technique. The mass spectrometric method described herein enables the concurrent measurement of peptides representative of a plurality of targeted PTH variants in a single assay.

US Patent:

20120064558, Mar 15, 2012

Filed:

Sep 13, 2010

Appl. No.:

12/881115

Inventors:

Mary Frances LOPEZ - Boston MA, US
Amol PRAKASH - Cambridge MA, US
David A. SARRACINO - Andover MA, US
Taha REZAI - Cambridge MA, US
Bryan E. KRASTINS - Somerville MA, US
Michael J. ATHANAS - Wayland MA, US

International Classification:

C12Q 1/37

US Classification:

435 23

Abstract:

Methods are disclosed for rapidly and reliably differentiating between ischemic and hemorrhagic stroke events in a human patient. The methods include obtaining a biological sample, such as blood serum, and analyzing the sample or a derivative thereof by mass spectrometry to measure the amount of one or more targeted proteins differentially present and ischemic and hemorrhagic stroke patients. The analysis may include proteolytic digestion of the biological sample and selective reaction monitoring (SRM) tandem mass spectrometry of the digested sample to determine the amounts of surrogate peptides corresponding to the targeted proteins.

US Patent:

20130217058, Aug 22, 2013

Filed:

Feb 25, 2013

Appl. No.:

13/776449

Inventors:

Mary Frances LOPEZ - Boston MA, US
David A. SARRACINO - Andover MA, US
Amol PRAKASH - Cambridge MA, US
Randall W. NELSON - Phoenix AZ, US
Paul ORAN - Scottsdale AZ, US
Bryan E. KRASTINS - Somerville MA, US
Taha REZAI - Clovis CA, US

International Classification:

G01N 27/62

US Classification:

435 23

Abstract:

Methods are described for monitoring the amounts of PTH variants in a biological sample by digesting the sample to produce surrogate peptides specific to the targeted PTH variants, and detecting and quantifying the surrogate peptides by selective reaction monitoring (SRM) mass spectrometry, using a set of precursor-to-product ion transitions optimized for sensitivity and selectivity. The PTH variants, or a portion thereof, may be concentrated in the sample by means of immunoaffinity capture or other suitable technique. The mass spectrometric method described herein enables the concurrent measurement of peptides representative of a plurality of targeted PTH variants in a single assay.

US Patent:

7652129, Jan 26, 2010

Filed:

Aug 13, 2004

Appl. No.:

10/568215

Inventors:

Mary Lopez - Bedford MA, US

Assignee:

PerkinElmer LAS, Inc. - Boston MA

International Classification:

A23J 1/00

US Classification:

530412

Abstract:

A sample is divided into a series of aliquots with the aliquots being subjected to at least two successive parallel separation steps in order to resolve protein or viral components thereof. The separation steps are performed not only on a sample but subsamples each containing a prelabeled tag to afford comparisons between subsamples. The parallel separation is amenable to high throughput and automation.