Matt S Anderson

US Patent:

7270971, Sep 18, 2007

Filed:

Oct 15, 2004

Appl. No.:

10/966628

Inventors:

Matt S. Anderson - Berkeley Heights NJ, US

Assignee:

Merck & Co., Inc. - Rahway NJ

International Classification:

C12Q 1/60

US Classification:

435 11

Abstract:

An improved method of obtaining fluorescence measurements which may be used as a measure of the CETP-catalyzed rate of transfer of a lipophilic non-polar fluorescent cholesteryl ester between lipoprotein particles is disclosed. The method may be used in assays for screening CETP inhibitors.

US Patent:

20060147934, Jul 6, 2006

Filed:

Feb 2, 2004

Appl. No.:

10/544700

Inventors:

Matt Anderson - ChathamTownship NJ, US
Sheryl Hyland - Rahway NJ, US

International Classification:

C12Q 1/68
C12Q 1/48

US Classification:

435006000, 435015000

Abstract:

The polyprenyl phosphate: N-acetylhexosamine-1-phosphate transferase family are glycosyl transferase enzymes of interest in the development of antibacterial treatments. The bacterial proteins WecA and MraY are exemplary transferases, each of which catalyzes the transfer of a specific hexosamine 1-P from a soluble UDP-hexosamine substrate to a polyprenyl phosphate carrier at the membrane surface. The present invention provides a generalizable, high throughput, one-pot assay for this type of enzymatic activity by incorporating a solid-liquid, bead-based separation system to selectively adsorb the highly hydrophobic products of the reaction. By judicious choice of radiolabeled UDP-hexosamine precursor, the assay format can be used to quantitate the products of diverse members of this transferase family as well as enzymes that catalyze the further modification of these transferase products. Thus, the use of this flexible assay format allows biochemical and enzymologic analysis of many such membrane bound transferases.