Matthew D Richards

US Patent:

20210163995, Jun 3, 2021

Filed:

Nov 25, 2020

Appl. No.:

17/105100

Inventors:

- Budapest, HU
- Ludwigshafen, DE
- San Diego CA, US
Matthew B. Richards - Lebanon NH, US
Tera Stoughtenger - New Lisbon WI, US
Kenneth Barrett - San Diego CA, US

International Classification:

C12P 7/06
C12N 1/18
C12N 9/60

Abstract:

The present disclosure concerns a process for fermenting a biomass with a reduced dose of a purified exogenous enzyme (which can be, for example a purified exogenous glucoamylase). The process comprises contacting a biomass (which may comprise starch) with a recombinant yeast host cell. The recombinant yeast host cell has a genetic modification for expressing a heterologous polypeptide having starch or dextrin hydrolase activity (which may be, for example, from a glucoamylase). The nucleic acid molecule encoding the heterologous polypeptide having starch or dextrin hydrolase activity comprises allowing the secretion of the heterologous polypeptide.