Matthew J Simpson

US Patent:

20180142249, May 24, 2018

Filed:

Oct 30, 2017

Appl. No.:

15/797285

Inventors:

- Indianapolis IN, US
Joseph F. Petolino - Zionsville IN, US
Andrew F. Worden - Indianapolis IN, US
Pierluigi Barone - Zionsville IN, US
Matthew A. Simpson - Brownsburg IN, US
Tonya L. Strange - Brownsburg IN, US

Assignee:

Dow AgroSciences LLC - Indianapolis IN

International Classification:

C12N 15/82

Abstract:

Compositions and methods to modify at least one target locus in a plant cell are provided, which comprises providing a plant cell, a plant, or a plant part with one or more target loci and one or more donor loci, providing at least one cleaving site specific nuclease to produce a double strand break within the target loci, followed by non-homologous end joining of at least one donor locus within at least one target locus. Target loci, donor loci and nuclease loci used in these methods, and plant cells, plants and plant parts comprising these target loci, donor loci, nuclease loci and/or the recombined loci are also provided.

US Patent:

20170283865, Oct 5, 2017

Filed:

Aug 29, 2016

Appl. No.:

15/249684

Inventors:

- Indianapolis IN, US
Matthew A. Simpson - Brownsburg IN, US
Zehui Cao - Westfield IN, US
Wei Chen - Carmel IN, US
Ning Zhou - Zionsville IN, US
Steven R. Webb - Westfield IN, US

Assignee:

Dow AgroSciences LLC - Indianapolis IN

International Classification:

C12Q 1/68
A01G 31/00
C12N 15/70
C12N 15/66
C40B 40/08
C12N 15/10

Abstract:

The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

US Patent:

20150307889, Oct 29, 2015

Filed:

Apr 24, 2015

Appl. No.:

14/695434

Inventors:

- Indianapolis IN, US
Tonya L. Strange - Brownsburg IN, US
Jayakumar Pon Samuel - Indianapolis IN, US
Ryan C. Blue - Fishers IN, US
Matthew A. Simpson - Brownsburg IN, US

International Classification:

C12N 15/82

Abstract:

Disclosed are methods for transformation of an androgenic-derived, haploid cell line with a site-specific nuclease. In some embodiments, the androgenic-derived, haploid cell line is a maize microspore-derived plant tissue culture. In addition, the disclosure provides a method for modifying, e.g., by mutating or targeting and integrating donor DNA into, a specific locus of a haploid or dihaploid tissue genome. The disclosure further provides methods for regenerating a whole plant from the haploid or dihaploid tissue that contains either the mutation at a specific genomic locus or a donor DNA integrated within a specific genomic locus may be obtained from the subject disclosure.

US Patent:

20140298547, Oct 2, 2014

Filed:

Dec 13, 2013

Appl. No.:

14/105223

Inventors:

- Indianapolis IN, US
Matthew Simpson - Brownsburg IN, US
Zehui Cao - Westfield IN, US
Wei Chen - Carmel IN, US
Ning Zhou - Zionsville IN, US
Steven R. Webb - Westfield IN, US

Assignee:

DOW AGROSCIENCES LLC - Indianapolis IN

International Classification:

C12Q 1/68

US Classification:

800306, 435 611, 506 9, 800298, 800312, 800314

Abstract:

The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.