Matthew R Yee

US Patent:

20190187140, Jun 20, 2019

Filed:

Aug 21, 2017

Appl. No.:

16/326687

Inventors:

- Oakland CA, US
Benjamin Ming Wu - San Marino CA, US
David Yuan Pereira - Los Angeles CA, US
Chloe Michelle Wu - San Marino CA, US
Matthew Foosing Yee - Saratoga CA, US
Joshua Anthony Keefe - Sunnyvale CA, US
Christina Caroline Pearce - Orinda CA, US
Amir Adam Dailamy - Calabasas CA, US
Vincent K. Wong - San Gabriel CA, US
Nguyen Khoi Le - Sacramento CA, US

International Classification:

G01N 33/559
G01N 33/543
G01N 33/545
G01N 33/569
G01N 33/487

Abstract:

It was discovered that hydrogel scaffolds can be used to induce phase separation as aqueous two-phase systems (ATPSs) pass through and/or rehydrate the scaffolds, allowing for concentration of target analyte(s) (e.g., biomolecule(s)) into a particular phase of the ATPS or into a leading front. Accordingly, in various embodiments methods and devices are provided that utilize aqueous two-phase systems and hydrogel scaffolds to improve the sensitivity of assays (e.g., of point-of-care assays) without sacrificing cost or ease of use.