Michael S Byrne

US Patent:

6462809, Oct 8, 2002

Filed:

Nov 12, 1999

Appl. No.:

09/439876

Inventors:

Thomas E. Ryan - Batavia NY
Michael J. Byrne - East Aurora NY

Assignee:

Leica Microsystems, Inc. - Buffalo NY

International Classification:

G01N 2141

US Classification:

356128, 356136

Abstract:

A sensor apparatus and associated method for sensing and monitoring specific binding of analyte to an immobilized binding layer are disclosed. The apparatus preferably comprises an automatic critical angle refractometer having a linear scanned array and an optical system for illuminating a portion of the array, which illumination depends upon the refractive index of the binding layer deposited on an optically transparent element. The apparatus further includes a flow cell for bringing the analyte in contact with the binding layer. The apparatus also includes a computer for receiving and processing refractive index data from the critical angle refractometer during the reaction between the analyte and the layer, which computer may be peripherally connected to the refractometer or enclosed within the refractometer housing. A preferred sensing method of the present invention generally comprises providing a critical angle refractometer generating light impinging upon the immobilized binding layer, contacting the binding layer with a contacting phase, measuring the critical angle of total reflection, which measurements are indicative of the presence or absence of interactions between the analyte and the binding layer.

US Patent:

6717663, Apr 6, 2004

Filed:

Mar 8, 2002

Appl. No.:

10/094213

Inventors:

Robert C. Atkinson - Buffalo NY
Thomas E. Ryan - Batavia NY
Keshav Sharma - Lancaster NY
Michael J. Byrne - East Aurora NY

Assignee:

Reichert, Inc. - Depew NY

International Classification:

G01N 2141

US Classification:

356136

Abstract:

An optical configuration for differential refractive index measurements of a test sample relative to a reference sample comprises an optical path along which an illumination beam travels to simultaneously illuminate a pair of optical interfaces on opposite sides of a meridional plane corresponding to the test sample and reference sample, respectively. Partial beams leaving the optical interfaces are optically diverged to illuminate different segments of a linear scanned array aligned in the meridional plane. The difference in location of a pair of shadowlines or a pair of resonance minimums formed by the partial beams on the array provides an indication of the refractive index difference.

US Patent:

6734956, May 11, 2004

Filed:

May 6, 2002

Appl. No.:

10/139577

Inventors:

Michael J. Byrne - East Aurora NY
Keshav D. Sharma - Lancaster NY
Robert C. Atkinson - Buffalo NY
Bruce R. Cordier - West Seneca NY

Assignee:

Reichert, Inc. - Depew NY

International Classification:

G01N 2141

US Classification:

356128, 356135

Abstract:

An optical configuration for measuring a difference in refractive index between a first sample and a second sample comprises partitioned first and second optical interfaces symmetrically illuminated by an illumination beam to provide first and second partial beams defined by the refractive index of the first and second samples, respectively. A linear scanned array is aligned in a meridional plane of the optical configuration for detection purposes, and an optical multiplexor is provided upstream of the linear scanned array for receiving the first and second partial beams and defining first and second optical channels carrying optical signal information corresponding to the first and second partial beams. The optical multiplexor switches between optical channels, such that the linear scanned array detects either the first or second optical channel at a given time. Thus, differential measurements are possible using a single linear array.

US Patent:

6869787, Mar 22, 2005

Filed:

Feb 27, 2002

Appl. No.:

10/083336

Inventors:

Mark A. Olson - Middletown MD, US
Charles B. Millard - Frederick MD, US
Michael P. Byrne - New Market MD, US
Robert W. Wannemacher - Frederick MD, US
Ross D. LeClaire - Frederick MD, US

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

C12N009/00
C12N009/24
C12N009/26

US Classification:

435201, 435 4, 435 6, 435 691, 435183, 435194, 435195, 435200, 4352523, 4353201, 530350, 536 232 T

Abstract:

Disclosed herein are polypeptides and variants thereof comprising a polypeptide sequence having substantial identity to ricin A chain (RTA) that lack detectable N-glycosidase-rRNA activity or exhibit reduced N-glycosidase-rRNA activity as compared to controls and methods of making and using thereof. The polypeptides and variants have a greater solubility in aqueous solutions of physiological pH and ionic strength than RTA and also retain the integrity of the neutralizing immunological epitope of wild type RTA. Also disclosed are immunogenic compositions that may be used to immunize a subject against ricin intoxication. Methods of immunizing against, treating, and preventing ricin intoxication are disclosed.

US Patent:

7081529, Jul 25, 2006

Filed:

Jul 20, 2001

Appl. No.:

09/910186

Inventors:

Leonard A. Smith - Clarksburg MD, US
Michael P. Byrne - New Market MD, US
John L. Middlebrook - Middletown MD, US
Hugh Lapenotiere - Charlestown WV, US
Michael A. Clayton - Mt. Airy MD, US
Douglas R. Brown - Githersburg MD, US

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

C07H 21/04

US Classification:

536 237, 514 44, 435 691

Abstract:

This invention is directed to preparation and expression of synthetic genes encoding polypeptides containing protective epitopes of botulinum neurotoxin (BoNT). The invention is also directed to production of immunogenic peptides encoded by the synthetic genes, as weel as recovery and purification of the immunogenic peptides from recombinant organisms. The invention is also directed to methods of vaccination against botulism using the expressed peptides.

US Patent:

7214787, May 8, 2007

Filed:

Jul 6, 2000

Appl. No.:

09/611419

Inventors:

Leonard A. Smith - Clarksburg MD, US
Michael P. Byrne - New Market MD, US
John L. Middlebrook - Middletown MD, US
Hugh Lapenotiere - Charlestown WV, US
Michael A. Clayton - Mt. Airy MD, US
Douglas R. Brown - Gaithersburg MD, US

Assignee:

United States of America as Represented by the Secretary of the Army - Washington DC

International Classification:

C07H 21/04

US Classification:

536 237, 536 231, 514 44, 435 711, 435 712, 435 691

Abstract:

This invention is directed to preparation and expression of synthetic genes encoding polypeptides containing protective epitopes of botulinum neurotoxin (BoNT). The invention is also directed to production of immunogenic peptides encoded by the synthetic genes, as weel as recovery and purification of the immunogenic peptides from recombinant organisms. The invention is also directed to methods of vaccination against botulism using the expressed peptides.

US Patent:

7407935, Aug 5, 2008

Filed:

Aug 23, 2004

Appl. No.:

10/923022

Inventors:

Mark A. Olson - Middletown MD, US
Charles B. Millard - Frederick MD, US
Michael P. Byrne - New Market MD, US
Robert W. Wannemacher - Frederick MD, US

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

A61K 38/16
A61K 36/47
C12P 21/02

US Classification:

514 12, 350370, 435 691

Abstract:

Disclosed herein are polypeptides and variants thereof comprising a polypeptide sequence having substantial identity to ricin A chain (RTA) that lack detectable N-glycosidase-rRNA activity or exhibit reduced N-glycosidase-rRNA activity as compared to controls and methods of making and using thereof. The polypeptides and variants have a greater solubility in aqueous solutions of physiological pH and ionic strength than RTA and also retain the integrity of the neutralizing immunological epitope of wild type RTA. Also disclosed are immunogenic compositions that may be used to immunize a subject against ricin intoxication. Methods of immunizing against, treating, and preventing ricin intoxication are disclosed.

US Patent:

7786285, Aug 31, 2010

Filed:

May 19, 2006

Appl. No.:

11/437212

Inventors:

Leonard A. Smith - Clarksburg MD, US
Michael P. Byrne - New Market MD, US
John L. Middlebrook - Middletown MD, US
Hugh Lapenotiere - Charlestown WV, US
Michael A. Clayton - Mt. Airy MD, US
Douglas R. Brown - Gaithersburg MD, US

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

C07H 21/02
C12N 15/09

US Classification:

536 237, 536 241, 435 691, 435 693, 435 697, 4352521, 4352523, 43525233, 4353201

Abstract:

This invention is directed to preparation and expression of synthetic genes encoding polypeptides containing protective epitopes of botulinum neurotoxin (BoNT). The invention is also directed to production of immunogenic peptides encoded by the synthetic genes, as well as recovery and purification of the immunogenic peptides from recombinant organisms. The invention is also directed to methods of vaccination against botulism using the expressed peptides.