Michael H Cardone

US Patent:

6730492, May 4, 2004

Filed:

Mar 9, 2002

Appl. No.:

10/093945

Inventors:

Michael H. Cardone - Boston MA
Michael Yaffe - Jamaica Plain MA

Assignee:

Merrimack Pharmaceuticals, Inc. - Cambridge MA

International Classification:

C12Q 148

US Classification:

435 15, 4353201, 435 6, 4352523, 435194, 435 691, 530350

Abstract:

Cell-based screening methods for determining kinase activity are provided. The methods utilize existing cellular pathways that are regulated by kinases. In one embodiment, various components of a ubiquitin-mediated degradation pathway are modified to create an assay that can be used to screen for a molecule that modulates the activity of a kinase of interest that otherwise does not regulate the degradation pathway. In another embodiment, various components of a protein translocation pathway are modified to screen for a molecule that modulates the activity of a kinase of interest that otherwise does not regulate the translocation pathway.

US Patent:

7083938, Aug 1, 2006

Filed:

Feb 3, 2004

Appl. No.:

10/771035

Inventors:

Michael H. Cardone - Boston MA, US
Michael Yaffe - Jamaica Plain MA, US

Assignee:

Merrimack Pharmaceuticals, Inc. - Cambridge MA

International Classification:

C12Q 1/48
C12N 9/00
C12P 21/06
C07K 1/00

US Classification:

435 15, 435183, 435 691, 530350

Abstract:

Cell-based screening methods for determining kinase activity are provided. The methods utilize existing cellular pathways that are regulated by kinases. In one embodiment, various components of a ubiquitin-mediated degradation pathway are modified to create an assay that can be used to screen for a molecule that modulates the activity of a kinase of interest that otherwise does not regulate the degradation pathway. In another embodiment, various components of a protein translocation pathway are modified to screen for a molecule that modulates the activity of a kinase of interest that otherwise does not regulate the translocation pathway.

US Patent:

8168755, May 1, 2012

Filed:

May 7, 2009

Appl. No.:

12/437127

Inventors:

Michael H. Cardone - Dorchester MA, US
Anthony G. Letai - Medfield MA, US

Assignee:

Eutropics Pharmaceuticals, Inc. - Cambridge MA

International Classification:

C07K 16/00
C12P 21/08

US Classification:

5303871, 5303881, 5303891

Abstract:

Isolated antibodies specifically binding to heterodimers of the Bcl-2 family and uses thereof for detecting presence of Bcl-2 heterodimers in a patient.

US Patent:

20020076727, Jun 20, 2002

Filed:

Aug 3, 2001

Appl. No.:

09/921655

Inventors:

Michael Cardone - Boston MA, US
Ulrik Nielsen - Cambridge MA, US
Gavin MacBeath - Arlington MA, US
James Marks - San Francisco CA, US
Peter Sorger - Cambridge MA, US
Anthony Sinsky - Boston MA, US

International Classification:

A61L002/00
B05D003/00
G01N033/53

US Classification:

435/007100, 427/002100

Abstract:

Disclosed are products and methods to facilitate the identification of compounds that are capable of interacting with biological macromolecules of interest, especially when such macromolecules are attached to a support surface in microarray. Aspects of the invention concern attachment chemistry, peptide labeling, antibody preparation, applications and so on.

US Patent:

20020102617, Aug 1, 2002

Filed:

Aug 3, 2001

Appl. No.:

09/923243

Inventors:

Gavin MacBeath - Arlington MA, US
Stuart Schreiber - Boston MA, US
Peter Sorger - Cambridge MA, US
Michael Cardone - Boston MA, US
John Newman - Cambridge MA, US

International Classification:

G01N033/53
G01N033/542
C12M001/34

US Classification:

435/007900, 435/287200

Abstract:

Driven by the influx of data from genome sequencing projects, systematic efforts are now underway to construct defined sets of cloned genes for high throughput expression and purification of recombinant proteins. To facilitate the subsequent study of protein function, the present invention provides protein microarrays that are compatible with the demand for extremely low sample volume and the rapid, simultaneous processing of thousands of proteins, and methods of assaying these arrays. The proteins are covalently or non-covalently attached to the surface of a solid support and retain their ability to interact specifically with other proteins, polynucleotides, other biological macromolecules, or small molecules.

US Patent:

20030170611, Sep 11, 2003

Filed:

Mar 9, 2002

Appl. No.:

10/093840

Inventors:

Michael Cardone - Boston MA, US
Michael Yaffe - Jamaica Plain MA, US

International Classification:

C12Q001/70
G01N033/53
C12Q001/48

US Classification:

435/005000, 435/007100, 435/015000

Abstract:

Cell-based screening methods for determining kinase activity are provided. The methods utilize existing cellular pathways that are regulated by kinases. In one embodiment, various components of a ubiquitin-mediated degradation pathway are modified to create an assay that can be used to screen for a molecule that modulates the activity of a kinase of interest that otherwise does not regulate the degradation pathway. In another embodiment, various components of a protein translocation pathway are modified to screen for a molecule that modulates the activity of a kinase of interest that otherwise does not regulate the translocation pathway.

US Patent:

20030170850, Sep 11, 2003

Filed:

Mar 9, 2002

Appl. No.:

10/093838

Inventors:

Michael Cardone - Boston MA, US
Michael Yaffe - Jamaica Plain MA, US

International Classification:

C12Q001/66
C12N009/12
C07H021/04
C12P021/02
C12N005/06

US Classification:

435/194000, 435/069700, 435/320100, 435/325000, 536/023200

Abstract:

Cell-based screening methods for determining kinase activity are provided. The methods utilize existing cellular pathways that are regulated by kinases. In one embodiment, various components of a ubiquitin-mediated degradation pathway are modified to create an assay that can be used to screen for a molecule that modulates the activity of a kinase of interest that otherwise does not regulate the degradation pathway. In another embodiment, various components of a protein translocation pathway are modified to screen for a molecule that modulates the activity of a kinase of interest that otherwise does not regulate the translocation pathway.

US Patent:

20040038428, Feb 26, 2004

Filed:

Apr 1, 2003

Appl. No.:

10/404619

Inventors:

Gavin MacBeath - Arlington MA, US
Stuart Schreiber - Boston MA, US
Peter Sorger - Cambridge MA, US
Michael Cardone - Boston MA, US
John Newman - Cambridge MA, US

International Classification:

G01N033/543

US Classification:

436/518000

Abstract:

Driven by the influx of data from genome sequencing projects, systematic efforts are now underway to construct defined sets of cloned genes for high throughput expression and purification of recombinant proteins. To facilitate the subsequent study of protein function, the present invention provides protein microarrays that are compatible with the demand for extremely low sample volume and the rapid, simultaneous processing of thousands of proteins, and methods of assaying these arrays. The proteins are covalently or non-covalently attached to the surface of a solid support and retain their ability to interact specifically with other proteins, polynucleotides, other biological macromolecules, or small molecules.