Michael M Delaney

US Patent:

8026349, Sep 27, 2011

Filed:

Aug 18, 2005

Appl. No.:

11/660589

Inventors:

Stephanie A. Hartsel - Berthoud CO, US
Robert J. Kaiser - Broomfield CO, US
Michael O. Delaney - Firestone CO, US

Assignee:

Dharmacon, Inc. - Lafayette CO

International Classification:

C07H 21/00
C07H 21/04

US Classification:

536 231, 536 253, 560190

Abstract:

Methods and compositions for making nucleoside phosphoramidites and nucleic acids, including mono-, di-, and polynucleotides, comprising a linker covalently attached to a levulinyl moiety are provided. A levulinyl-protected linking moiety affords an orthogonal approach to modifying a polynucleotide during or after solid phase synthesis with a molecule of interest, for example, a conjugate or a dye.

US Patent:

20070269889, Nov 22, 2007

Filed:

Feb 4, 2005

Appl. No.:

11/051195

Inventors:

Devin Leake - Denver CO, US
Anastasia Khvorova - Boulder CO, US
Yuriy Fedorov - Superior CO, US
Michael Delaney - Firestone CO, US
Barbara Robertson - Boulder CO, US
Emily Anderson - Lafayette CO, US
Angela Reynolds - Conifer CO, US

Assignee:

DHARMACON, INC. - Lafayette CO

International Classification:

C07H 21/04

US Classification:

435375000, 536024100

Abstract:

RNA molecules, including siRNA molecules and related control, trackability and exaequo agents with specific stability modifications are provided. These molecules are particularly advantageous as transfection control reagents. The molecules include first and second 5′ terminal sense nucleotides with 2′-O-alkyl groups and a label on the first 5′ terminal sense nucleotide, in conjunction with at least one additional 2′-O-alkyl pyrimidine modified sense nucleotide, and either: (i) at least one 2′ fluoro modified pyrimidine antisense nucleotide and a phosphorylated first 5′ terminal antisense nucleotide; or (ii) a first and second 5′ terminal antisense nucleotide with 2′-O-alkyl modifications and at least one additional 2′-O-alkyl pyrimidine modified antisense nucleotide.

US Patent:

20090280567, Nov 12, 2009

Filed:

Jun 17, 2009

Appl. No.:

12/486497

Inventors:

Devin Leake - Denver CO, US
Anastasia Khvorova - Boulder CO, US
Yuriy Fedorov - Superior CO, US
Michael Owen Delaney - Dacono CO, US
Barbara Robertson - Boulder CO, US
Emily Anderson - Lafayette CO, US
Angela Reynolds - Conifer CO, US

Assignee:

DHARMACON, INC. - Lafayette CO

International Classification:

C12N 15/87
C07H 21/02

US Classification:

435440, 536 241

Abstract:

RNA molecules, including siRNA molecules and related control, trackability and exaequo agents with specific stability modifications are provided. These molecules are particularly advantageous as transfection control reagents. The molecules include first and second 5′ terminal sense nucleotides with 2′-O-alkyl groups and a label on the first 5′ terminal sense nucleotide, in conjunction with at least one additional 2′-O-alkyl pyrimidine modified sense nucleotide, and either: (i) at least one 2′ fluoro modified pyrimidine antisense nucleotide and a phosphorylated first 5′ terminal antisense nucleotide; or (ii) a first and second 5′ terminal antisense nucleotide with 2′-O-alkyl modifications and at least one additional 2′-O-alkyl pyrimidine modified antisense nucleotide.

US Patent:

20100216984, Aug 26, 2010

Filed:

May 16, 2008

Appl. No.:

12/600829

Inventors:

Michael Oren Delaney - Dacono CO, US

Assignee:

Dharmacon, Inc. - Lafayette CO

International Classification:

C07H 19/00
C07F 7/12
C07D 249/04

US Classification:

536 2532, 556488, 548255, 536 221

Abstract:

The present invention provides compounds of the formula (I): C-Q-O—Si(R)(R)—N wherein C is a chromophore; Q is selected from the group consisting of optionally substituted aliphatic, aryl, heteroaryl, cycloalkyl or heterocycloalkyl; Rand Rare independently selected from the group consisting of optionally substituted Calkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, Calkyloxy, cycloalkyloxy, heterocycloalkyloxy, alkylsilyloxy and arylsilyloxy; and N is a glycosylamine or abasic moiety.

US Patent:

20180142236, May 24, 2018

Filed:

Apr 7, 2016

Appl. No.:

15/571532

Inventors:

- Wauwatosa WI, US
Emily Marie Anderson - Lafayette CO, US
Michael Oren Delaney - Lafayette CO, US

International Classification:

C12N 15/11
C12N 9/22

Abstract:

The present invention provides synthetic single guide RNAs that comprise two separate functional sequences (commonly known as crRNA and tracrRNA) connected by a linker. These synthetic single guide RNA molecules are useful in gene editing when used with RNA-guided endonucleases such as cas9 in eukaryotic cells. The availability of the synthetic single guide RNAs makes the screening for gene editing in high-through-put format simple and convenient.