A sandwich immunoassay is disclosed that provides simple to perform yet sensitive identification of analytes in samples. All assay constituents needed (except analyte to be detected) for one assay are dried. Upon reconstitution with sample, a 10 to 15 minute incubation gives a rapid and convenient detection assay capability. The method incorporates the capture of antigen to an immobilized capture antibody. A labeled reporter antibody with the molecule, binds to the antigen to form an immunocomplex capable of generating a detectable signal.
Immunoassay And Reagents And Kits For Performing The Same
Deborah Gatto-Menking - Bel Air MD, US Michael goode - Baltimore MD, US
Assignee:
Science and Technology Corp. - Hampton VA
International Classification:
G01N 33/53 C07K 16/46
US Classification:
435007930, 530391100
Abstract:
A sandwich immunoassay is disclosed that provides simple to perform yet sensitive identification of analytes in samples. All assay constituents needed (except analyte to be detected) for one assay are dried. Upon reconstitution with sample, a 10 to 15 minute incubation gives a rapid and convenient detection assay capability. The method incorporates the capture of antigen to an immobilized capture antibody. A labeled reporter antibody with the molecule, binds to the antigen to form an immunocomplex capable of generating a detectable signal.
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Ohio University - Biological Sciences and Chemistry
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