Michael S Knapp

US Patent:

6537748, Mar 25, 2003

Filed:

Feb 26, 1999

Appl. No.:

09/258133

Inventors:

Philip Goelet - Cockeysville MD
Michael R. Knapp - Baltimore MD
Stephen Anderson - Princeton NJ

Assignee:

Orchid BioSciences, Inc. - Princeton NJ

International Classification:

C12Q 168

US Classification:

435 6, 435 5, 435 912, 536 231, 536 2433, 536 261, 536 2612

Abstract:

This invention concerns a reagent composition comprising at least two different terminators of a nucleic acid template-dependent, primer extension reaction. This invention also concerns a method for determining the identity of a nucleotide base at a specific position in a nucleic acid of interest. This invention further concerns a method for determining the presence or absence of a particular nucleotide sequence in a sample of nucleic acids. This invention further concerns a method for identifying different alleles in a sample containing nucleic acids. This invention further concerns a method for determining the genotype of an organism at one or more particular genetic loci.

US Patent:

7585466, Sep 8, 2009

Filed:

Jul 18, 2000

Appl. No.:

09/618178

Inventors:

Stephen E. Lincoln - Cockeysville MD, US
Michael R. Knapp - Baltimore MD, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

G01N 21/25
G01N 21/29

US Classification:

422 8208, 422 67, 435 6

Abstract:

Genotypes of a subject may be determined by analyzing a biological sample. Measurements of the intensity of allele specific signals may be obtained and used to generate reaction values. These reaction values may be compared to probability distributions in order to obtain conditional probabilities of genotypes of interest. The genotype of the subject may be determined based on the measures of these conditional probabilities.

US Patent:

20030044778, Mar 6, 2003

Filed:

Feb 26, 1999

Appl. No.:

09/258132

Inventors:

PHILIP GOELET - COCKEYSVILLE MD, US
MICHAEL R. KNAPP - BALTIMORE MD, US
STEPHEN ANDERSON - PRINCETON NJ, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

This invention concerns a reagent composition comprising at least two different terminators of a nucleic acid template-dependent, primer extension reaction. This invention also concerns a method for determining the identity of a nucleotide base at a specific position in a nucleic acid of interest. This invention further concerns a method for determining the presence or absence of a particular nucleotide sequence in a sample of nucleic acids. This invention further concerns a method for identifying different alleles in a sample containing nucleic acids. This invention further concerns a method for determining the genotype of an organism at one or more particular genetic loci.

US Patent:

20030170624, Sep 11, 2003

Filed:

May 1, 2001

Appl. No.:

09/846863

Inventors:

Philip Goelet - Cockeysville MD, US
Michael Knapp - Baltimore MD, US

International Classification:

C12Q001/68
C07H021/04
C12P019/34

US Classification:

435/006000, 435/091200, 536/024300

Abstract:

Molecules and methods suitable for identifying polymorphic sites in the genome of a plant or animal. The identification of such sites is useful in determining identity, ancestry, predisposition to genetic disease, the presence or absence of a desired trait, etc.

US Patent:

56102879, Mar 11, 1997

Filed:

Nov 16, 1994

Appl. No.:

8/341148

Inventors:

Theo Nikiforov - San Diego CA
Michael R. Knapp - Baltimore MD

Assignee:

Molecular Tool, Inc. - Baltimore MD

International Classification:

C07H 2104
C12Q 168
C12N 1500
C12N 1108

US Classification:

536 243

Abstract:

Synthetic nucleic acid molecules are non-covalently immobilized in the presence of a salt or cationic detergent on a hydrophilic polystyrene solid support containing an --OH, --C. dbd. O or --COOH hydrophilic group or on a glass solid support. The support is contacted with a solution having a pH of about 6 to about 8 containing the synthetic nucleic acid and the cationic detergent or salt. Preferably, the cationic detergent is 1-ethyl-3-(3'-dimethylaminopropyl)-1,3-carbodiimide hyrochloride at a concentration of about 30 mM to about 100 mM or octyldimethylamine hydrochloride at a concentration of about 50 mM to about 150 mM. The salt is preferably NaCl at a concentration of about 50 mM to about 250 mM. When the detergent is 1-ethyl-3-(3'-dimethylaminopropyl)-1,3-carbodiimide hyrochloride, the glass support or the hydrophilic polystyrene support is used. When NaCl or octyldimethylamine hydrochloride is used, the support is the hydrophilic polystyrene.

US Patent:

58888195, Mar 30, 1999

Filed:

Mar 5, 1991

Appl. No.:

7/664837

Inventors:

Philip Goelet - Cockeysville MD
Michael R. Knapp - Baltimore MD
Stephen Anderson - Princeton NJ

Assignee:

Molecular Tool, Inc. - Baltimore MD

International Classification:

C12Q 170
C12Q 168
C12P 1934

US Classification:

435 5

Abstract:

The invention concerns a reagent composition that employs at least two different terminators of a nucleic acid template-dependent primer extension reaction to determine the identity of a nucleotide base at a specific position in a nucleic acid of interest. The invention also concerns the method for determining such identification. The invention may be used to determine the presence or absence of a specific nucleotide sequence in a sample. It may also be employed in determination of genotype and in the identification of different alleles.

US Patent:

60047445, Dec 21, 1999

Filed:

Oct 11, 1991

Appl. No.:

7/775786

Inventors:

Philip Goelet - Cockeysville MD
Michael R. Knapp - Baltimore MD
Stephen Anderson - Princeton NJ

Assignee:

Molecular Tool, Inc. - Baltimore MD

International Classification:

C12Q 170
C12Q 168
C12P 1934
C07H 2104

US Classification:

435 5

Abstract:

The invention concerns a reagent composition that employs at least two different terminators of a nucleic acid template-dependent primer extension reaction to determine the identity of a nucleotide base at a specific position in a nucleic acid of interest. The invention also concerns an immobilized method for determining such identification. The invention may be used to determine the presence or absence of a specific nucleotide sequence in a sample. It may also be employed in determination of genotype and in the identification of different alleles.

US Patent:

55189008, May 21, 1996

Filed:

Nov 23, 1993

Appl. No.:

8/155746

Inventors:

Theo T. Nikiforov - Baltimore MD
Michael R. Knapp - Baltimore MD

Assignee:

Molecular Tool, Inc. - Baltimore MD

International Classification:

C12Q 168
C12P 1934
C07H 2104
C12N 1500

US Classification:

435 911

Abstract:

A method for generating single-stranded nucleic acid molecules. The molecules contain nuclease resistant modified nucleotides, such that they are resistant to 5'. fwdarw. 3' exonucleases.