Michael J Laird

US Patent:

7405059, Jul 29, 2008

Filed:

Jun 7, 2006

Appl. No.:

11/447892

Inventors:

Michael W. Laird - San Ramon CA, US

Assignee:

Human Genome Sciences, Inc. - Rockville MD

International Classification:

C12N 15/09
C12N 1/21

US Classification:

435 693, 4352523

Abstract:

Novel Shine-Dalgarno (ribosome binding site) sequences, vectors containing such sequences, and host cells transformed with these vectors are provided. Methods of use of such sequences, vectors, and host cells for the efficient production of proteins and fragments thereof in prokaryotic systems are also provided. In particular embodiments of the invention, compounds and methods for high efficiency production of soluble protein in prokaryotic systems are provided.

US Patent:

8574869, Nov 5, 2013

Filed:

Jan 19, 2012

Appl. No.:

13/354223

Inventors:

Yung-Hsiang Kao - San Mateo CA, US
Michael W. Laird - San Ramon CA, US
Melody Trexler Schmidt - San Carlos CA, US
Rita L. Wong - Redwood City CA, US
Daniel P. Hewitt - Sunnyvale CA, US

Assignee:

Genentech, Inc. - South San Francisco CA

International Classification:

C12P 1/00
C12N 5/02

US Classification:

435 41, 435325

Abstract:

Provided herein are methods for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

US Patent:

20030175208, Sep 18, 2003

Filed:

Oct 16, 2002

Appl. No.:

10/270487

Inventors:

Guo-Liang Yu - Berkeley CA, US
Reinhard Ebner - Gaithersburg MD, US
Jian Ni - Germantown MD, US
Craig Rosen - Laytonsville MD, US
Stephen Ullrich - Rockville MD, US
Michael Laird - Germantown MD, US

Assignee:

Human Genome Sciences, Inc. - Rockville MD

International Classification:

A61K051/00

US Classification:

424/001490, 424/001690

Abstract:

The present invention relates to nucleic acid molecules encoding Neutrokine-alpha and/or Neutrokine-alphaSV polypeptides, including soluble forms of the extracellular domain. Neutrokine-alpha and/or Neutrokine-alphaSV polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to antibodies or portions thereof that specifically bind Neutrokine-alpha and/or Neutrokine-alphaSV and diagnostic and therapeutic methods using these antibodies. Also provided are diagnostic methods for detecting immune system-related disorders and therapeutic methods for treating immune system-related disorders using the compositions of the invention.

US Patent:

20080274503, Nov 6, 2008

Filed:

Jun 19, 2008

Appl. No.:

12/142313

Inventors:

Michael W. Laird - San Ramon CA, US

Assignee:

Human Genome Sciences, Inc. - Rockville MD

International Classification:

C12P 21/04
C12N 15/11
C12N 15/00
C12N 15/63
C12N 1/20

US Classification:

435 691, 536 231, 4353201, 435471, 43525233

Abstract:

Novel Shine-Dalgarno (ribosome binding site) sequences, vectors containing such sequences, and host cells transformed with these vectors are provided. Methods of use of such sequences, vectors, and host cells for the efficient production of proteins and fragments thereof in prokaryotic systems are also provided. In particular embodiments of the invention, compounds and methods for high efficiency production of soluble protein in prokaryotic systems are provided.

US Patent:

20090053786, Feb 26, 2009

Filed:

Jul 8, 2008

Appl. No.:

12/217745

Inventors:

Yung-Hsiang Kao - San Mateo CA, US
Michael W. Laird - San Ramon CA, US
Melody Trexler Schmidt - San Carlos CA, US
Rita L. Wong - Redwood City CA, US
Daniel P. Hewitt - Sunnyvale CA, US

International Classification:

C12N 9/99
C12N 5/06

US Classification:

435184, 435375, 435358, 4352523, 43525233

Abstract:

The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

US Patent:

20210095040, Apr 1, 2021

Filed:

Dec 16, 2020

Appl. No.:

17/124314

Inventors:

- South San Francisco CA, US
Michael W. LAIRD - San Ramon CA, US
Melody Trexler SCHMIDT - Danville CA, US
Rita L. WONG - Redwood City CA, US
Daniel P. HEWITT - Sunnyvale CA, US

Assignee:

Genentech, Inc. - South San Francisco CA

International Classification:

C07K 16/28
A61K 39/395
C07K 1/14

Abstract:

The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

US Patent:

20210009988, Jan 14, 2021

Filed:

Sep 29, 2020

Appl. No.:

17/036075

Inventors:

- South San Francisco CA, US
Masaru Ken Shiratori - South San Francisco CA, US
Bradley Richard Snedecor - South San Francisco CA, US
Michael W. Laird - South San Francisco CA, US

Assignee:

GENENTECH, INC. - South San Francisco CA

International Classification:

C12N 15/10
C12N 5/071
C12N 15/113
C12N 9/22

Abstract:

The present disclosure relates to methods, cells and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interests, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have modulated lactogenic activity. The present disclosure also relates to methods and compositions for modulating pyruvate kinase muscle (PKM) expression (e.g., PKM-1 expression) in a mammalian cell to thereby reduce or eliminate the lactogenic activity of the cell, as well compositions comprising a cell having reduced or eliminated lactogenic activity and methods of using the same.

US Patent:

20200347145, Nov 5, 2020

Filed:

Apr 13, 2020

Appl. No.:

16/847317

Inventors:

- South San Francisco CA, US
Michael W. LAIRD - San Ramon CA, US
Melody Trexler SCHMIDT - Danville CA, US
Rita L. WONG - Redwood City CA, US
Daniel P. HEWITT - Sunnyvale CA, US

Assignee:

Genentech, Inc. - South San Francisco CA

International Classification:

C07K 16/28
A61K 39/395
C07K 1/14

Abstract:

The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.