Michael Pw Lin

US Patent:

20090203035, Aug 13, 2009

Filed:

Oct 27, 2008

Appl. No.:

12/259089

Inventors:

Nathan C. Shaner - Seaside CA, US
Michael Z. Lin - San Diego CA, US
Michael McKeown - La Jolla CA, US
Roger Y. Tsien - La Jolla CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

G01N 33/53
C07K 14/00
C07H 21/00
C12N 15/74
C12N 5/06
C12Q 1/02

US Classification:

435 71, 530350, 530402, 536 231, 4353201, 435325, 435 29

Abstract:

The present invention relates to novel fluorescent protein variants of DsRed and eqFP578. Fluorescent protein variants having increased photostability and/or having reversible photoswitching behavior, as well as polynucleotides encoding such variants are provided herein. Methods of using these novel fluorescent protein variants and methods for constructing other fluorescent protein variants having increased photostability are also provided by the present invention.

US Patent:

20100041092, Feb 18, 2010

Filed:

Sep 28, 2007

Appl. No.:

12/443411

Inventors:

Michael Z. Lin - San Diego CA, US
Roger Y. Tsien - La Jolla CA, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

C12Q 1/02
C07K 2/00
C12N 9/96
C07H 21/04
C12N 15/62
C12N 1/21

US Classification:

435 29, 530402, 435188, 536 234, 43525233, 536 231

Abstract:

The disclosure provides method and compositions for visualizing protein turnover. In particular, the disclosure provides methods and compositions useful for measuring the age of particular proteins or the dynamics of localized protein translation.