Michael A Moser

US Patent:

7514212, Apr 7, 2009

Filed:

Jan 17, 2004

Appl. No.:

10/542464

Inventors:

James R. Prudent - Madison WI, US
Scott C. Johnson - Sun Prairie WI, US
Michael J. Moser - Madison WI, US
David J. Marshall - Madison WI, US

Assignee:

EraGen Biosciences, Inc. - Madison WI

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 912

Abstract:

The present invention provides methods, kits and compositions for high fidelity nucleic acid amplification reactions of target nucleic acids such that specific amplification products that incorporate non-standard bases are produced. The amplification reactions can either be linear or exponential. In some embodiments, the fidelity of the amplification reaction is high enough such that a majority of the amplification products maintain non-standard bases at specific sites designated by the user after a specified number of amplification cycles. In some embodiments, the ratio of non-standard nucleoside triphosphates is greater than the amount of standard nucleoside triphosphates initially present in the reaction mixture.

US Patent:

7820808, Oct 26, 2010

Filed:

Apr 1, 2004

Appl. No.:

10/551705

Inventors:

Michael James Moser - Madison WI, US
David J. Marshall - Madison WI, US
James R. Prudent - Madison WI, US
Christopher V. Van Hout - Ann Arbor MI, US
Christine A. Larsen - Corvallis OR, US

Assignee:

EraGen Biosciences, Inc. - Madison WI

International Classification:

C07H 21/04
A61K 48/00

US Classification:

536 245, 536 231, 514 44

Abstract:

The present invention provides nucleic acid based polymerase inhibitors and methods for reducing non-specific polymerase extension and amplification in nucleic acid amplification reactions. The polymerase inhibitors provide a double stranded nucleic acid portion that is recognized by a polymerase enzyme as a template for extension but is incapable of being extended by the polymerase enzyme. The polymerase binds to the polymerase inhibitor which sequesters the enzyme until the temperature achieves a level that denatures the double stranded portion of the inhibitor after which the polymerase is released and can then catalyze nucleic acid extension.

US Patent:

8293472, Oct 23, 2012

Filed:

Jun 6, 2006

Appl. No.:

11/447734

Inventors:

Michael James Moser - Madison WI, US

Assignee:

Luminex Corporation - Austin TX

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 612, 435 61, 435 611

Abstract:

Disclosed are methods for identifying and characterizing polynucleotide sequences in a sample which may include a heterogeneous sample. Some of the methods are directed to the identification and characterization of a virus in a sample, which may include HIV capable of cause AIDS or AIDS-like symptoms. The virus may be HIV-1, and may also include drug resistant mutations. The methods may include reacting a mixture that includes, in addition to nucleic acid isolated from the sample, at least one oligonucleotide capable of specifically hybridizing to HIV nucleic acid where the oligonucleotide includes at least one non-natural base. In addition, the methods may include detection of one or more mutations in HIV nucleic acid that are associated with drug resistance.

US Patent:

8313932, Nov 20, 2012

Filed:

Sep 17, 2010

Appl. No.:

12/884575

Inventors:

Michael James Moser - Madison WI, US
David J. Marshall - Madison WI, US
James R. Prudent - Madison WI, US
Cristopher V. Van Hout - Ann Arbor MI, US
Christine A. Larsen - Corvallis OR, US

Assignee:

Luminex Corporation - Austin TX

International Classification:

C12P 19/34
A61K 48/00
C07H 21/04

US Classification:

435 912, 536 231, 536 245, 514 44

Abstract:

The present invention provides nucleic acid based polymerase inhibitors and methods for reducing non-specific polymerase extension and amplification in nucleic acid amplification reactions. The polymerase inhibitors provide a double stranded nucleic acid portion that is recognized by a polymerase enzyme as a template for extension but is incapable of being extended by the polymerase enzyme. The polymerase binds to the polymerase inhibitor which sequesters the enzyme until the temperature achieves a level that denatures the double stranded portion of the inhibitor after which the polymerase is released and can then catalyze nucleic acid extension.

US Patent:

20070059686, Mar 15, 2007

Filed:

Jul 28, 2006

Appl. No.:

11/494820

Inventors:

Michael Moser - Madison WI, US
James Prudent - Madison WI, US

International Classification:

C12Q 1/70
C12Q 1/68

US Classification:

435005000, 435006000

Abstract:

Disclosed are methods and kits for identifying a virus in a sample, which may include a coronavirus capable of causing Severe Acute Respiratory Syndrome (“SARS”) or SARS-like symptoms. The virus may be a SARS-Associated Coronavirus (“SARS-CoV”). Typically, the methods may include reacting a mixture that includes, in addition to nucleic acid isolated from the sample, at least one oligonucleotide capable of specifically hybridizing to SARS-CoV nucleic acid where the oligonucleotide includes at least one non-natural base. In addition, the mixture may include control nucleic acid.

US Patent:

20090275039, Nov 5, 2009

Filed:

Apr 16, 2009

Appl. No.:

12/425088

Inventors:

Michael J. Moser - Madison WI, US
Vecheslav A. Elagin - Waunakee WI, US

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

Disclosed are methods, kits, and components for detecting small RNA molecules, such as microRNA and siRNA, in a sample. The methods utilize primers and reporter molecules comprising non-natural bases. The disclosed kits may include one or more components for performing the disclosed methods.

US Patent:

20100086908, Apr 8, 2010

Filed:

May 16, 2008

Appl. No.:

12/122250

Inventors:

James R. Prudent - Madison WI, US
David J. Marshall - Madison WI, US
Wai-Ming Lee - Madison WI, US
James Gern - Madison WI, US
Michael J. Moser - Madison WI, US
Edward L. Beaty - Madison WI, US
Gerda Harms - Middleton WI, US

International Classification:

C12Q 1/70

US Classification:

435 5

Abstract:

The present invention relates generally to the field of nucleic acid detection and more specifically to the detection of human respiratory viruses in a patient sample. In some aspects, the invention relates to the detection of multiple respiratory viral groups, including rhinovirus, respiratory syncytial virus, parainfluenza virus, influenza virus, metapneumovirus, adenovirus, coronavirus, and enterovirus.

US Patent:

20120122095, May 17, 2012

Filed:

Jan 11, 2007

Appl. No.:

11/652702

Inventors:

Michael James Moser - Madison WI, US
David J. Marshall - Madison WI, US

International Classification:

C12Q 1/68
G01N 21/64

US Classification:

435 612, 435 615

Abstract:

Disclosed are methods and kits for identifying a virulent bacteria in a sample, which may include virulent bacteria belonging to the genus (e.g., , and ). Typically, the methods include (a) reacting a mixture that includes, in addition to nucleic acid isolated from the sample, (i) at least one oligonucleotide capable of specifically hybridizing to nucleic acid of plasmid pX01; and (ii) at least one oligonucleotide capable of specifically hybridizing to nucleic acid of plasmid pX02. In addition, the mixture may include control nucleic acid. In the methods, nucleic acid of plasmid pX01 and nucleic acid of plasmid pX02 are detected, and optionally control nucleic acid is detected, thereby identifying the virulent bacteria.