Michael L Nguyen

US Patent:

20220404353, Dec 22, 2022

Filed:

Nov 20, 2020

Appl. No.:

17/780227

Inventors:

- San Diego CA, US
Jim Chauvapun - San Diego CA, US
Mathias Ehrich - San Diego CA, US
Megan Monier - Corona CA, US
Michael Van Nguyen - San Diego CA, US
Dirk van den Boom - Encinitas CA, US

International Classification:

G01N 33/543
G01N 33/53
C12Q 1/6804
C12N 15/10

Abstract:

Provided are systems and methods of sample preparation and analyte detection.

US Patent:

20220380752, Dec 1, 2022

Filed:

May 25, 2022

Appl. No.:

17/824617

Inventors:

- San Diego CA, US
Mathias Ehrich - San Diego CA, US
Michael Van Nguyen - San Diego CA, US
Christopher Ellison - La Jolla CA, US
Jeffery William Domenighini - Encinitas CA, US
Dylann Duncan Ceriani - San Diego CA, US
Kristopher Scott Buchanan - Fort Collins CO, US
Michael Gordon Smith - Fort Collins CO, US

International Classification:

C12N 15/10
C12Q 1/6806

Abstract:

Provided herein are devices and methods for extracting blood plasma from ultra-low volumes of blood. In some cases, the devices and methods use positive pressure to force a blood sample through a filter or a membrane. In some cases, the devices and methods use centrifugation to force a blood sample through a filter or a membrane. The devices and methods disclosed herein are suitable for the preparation of high-quality samples containing cell-free nucleic acids.

US Patent:

20230065224, Mar 2, 2023

Filed:

Jan 28, 2021

Appl. No.:

17/795853

Inventors:

- San Diego CA, US
Kapil MAHAKALKAR - San Diego CA, US
Yi ZHANG - San Diego CA, US
Andrew Boyd MACCONNELL - San Diego CA, US
Joseph Franklin ROKICKI - San Diego CA, US
Michael VAN NGUYEN - San Diego CA, US

International Classification:

C12N 15/10
B01L 3/00

Abstract:

This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are a method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.

US Patent:

20210340609, Nov 4, 2021

Filed:

Mar 11, 2021

Appl. No.:

17/198932

Inventors:

- San Diego CA, US
Michael VAN NGUYEN - San Diego CA, US

Assignee:

Omniome, Inc. - San Diego CA

International Classification:

C12Q 1/6853
C12Q 1/6886
C12Q 1/6827
C12Q 1/686
C12Q 1/6883
C12Q 1/6872

Abstract:

A method for identifying target alleles, that includes steps of (a) forming a plurality of stabilized ternary complexes at a plurality of features on an array, wherein the stabilized ternary complexes each has a polymerase, a template nucleic acid having a target allele of a locus, a primer hybridized to the locus, and a next correct nucleotide having a cognate in the locus, wherein either (i) the primer is an allele-specific primer having a 3′ nucleotide that is a cognate nucleotide for the target allele, or (ii) the primer is a locus-specific primer and the next correct nucleotide hybridizes to the target allele; and (b) detecting stabilized ternary complexes at the features, thereby identifying the target alleles.

US Patent:

20210340612, Nov 4, 2021

Filed:

Mar 3, 2021

Appl. No.:

17/190894

Inventors:

- San Diego CA, US
Joseph ROKICKI - San Diego CA, US
Keunho AHN - San Diego CA, US
Brittany Ann ROHRMAN - San Diego CA, US
Michael NGUYEN - San Diego CA, US
Kandaswamy VIJAYAN - San Diego CA, US

Assignee:

Omniome, Inc. - San Diego CA

International Classification:

C12Q 1/6869
C12Q 1/6806
C07H 21/02
C07H 99/00
C07H 21/04
C12Q 1/6874

Abstract:

Method and composition for identifying cognate nucleotides in a Sequencing By Binding™ procedure, wherein one or more labeled nucleotides are detected in ternary complexes but never incorporated. Labeled nucleotides can be incorporable nucleotides that contact preformed blocked primed template nucleic acids. Alternatively, labeled nucleotides are labeled non-incorporable nucleotides. Labeled nucleotides, including labeled non-incorporable nucleotides, can be detected in ternary complexes in the same reaction mixture that incorporates a reversible terminator nucleotide to create a blocked primed template nucleic acid. Detection of ternary complexes can take place in the presence of a catalytic metal ion.

US Patent:

20210246491, Aug 12, 2021

Filed:

Apr 28, 2021

Appl. No.:

17/243481

Inventors:

- Carlsbad CA, US
Christine J. Shaw - San Diego CA, US
Milena Iacobelli Martinez - Vista CA, US
Anna Maria Al-Khouri - San Diego CA, US
Bradley Adam Brown - San Marcos CA, US
John Jay Harvey - San Marcos CA, US
Michael Thomas Nguyen - Carlsbad CA, US

Assignee:

Clinical Micro Sensors, Inc. (dba GenMark Diagnostics, Inc.) - Carlsbad CA

International Classification:

C12Q 1/689
C12Q 1/686
C12Q 1/6853
B01L 3/00
C12Q 1/6895
G01N 27/327
G01N 27/48

Abstract:

The present disclosure relates to methods and devices for amplifying a plurality of targets in a single PCR run while distinguishing between clinically relevant amplification and amplification from other sources such as from background contamination. The methods and devices further enable discrimination between gram-positive, gram-negative and fungal infections as wells as identify antimicrobial resistance genes. When applying the methods and devices of the invention, the species or genus of an infection(s), and genus of a fungal co-infection(s) or category of bacterial (gram-positive or negative) co-infection(s) are identified. Species identification of co-infections can also be achieved. Further, when applying the methods and devices of the invention, organisms which are likely to be contaminating organisms from a blood draw are identified.

US Patent:

20200324287, Oct 15, 2020

Filed:

May 8, 2020

Appl. No.:

16/870809

Inventors:

- San Diego CA, US
Andrew Boyd MACCONNELL - San Diego CA, US
Joseph Franklin ROKICKI - Del Mar CA, US
Michael VAN NGUYEN - San Diego CA, US

Assignee:

Plexium, Inc. - San Diego CA

International Classification:

B01L 3/00
C12N 15/10
C12Q 1/6869
C40B 50/04
C40B 50/14
C12Q 1/6876
C40B 30/06
C40B 50/16
C40B 30/04

Abstract:

This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.

US Patent:

20200140939, May 7, 2020

Filed:

Jan 7, 2020

Appl. No.:

16/736139

Inventors:

- San Diego CA, US
Michael VAN NGUYEN - San Diego CA, US

Assignee:

Omniome, Inc. - San Diego CA

International Classification:

C12Q 1/6853
C12Q 1/6883
C12Q 1/6872
C12Q 1/686
C12Q 1/6827
C12Q 1/6886

Abstract:

A method for identifying target alleles, that includes steps of (a) forming a plurality of stabilized ternary complexes at a plurality of features on an array, wherein the stabilized ternary complexes each has a polymerase, a template nucleic acid having a target allele of a locus, a primer hybridized to the locus, and a next correct nucleotide having a cognate in the locus, wherein either (i) the primer is an allele-specific primer having a 3′ nucleotide that is a cognate nucleotide for the target allele, or (ii) the primer is a locus-specific primer and the next correct nucleotide hybridizes to the target allele; and (b) detecting stabilized ternary complexes at the features, thereby identifying the target alleles.