A method for making a directed antisense library against a target transcript is described. A cDNA of the target transcript is cloned in an appropriate cloning vector. Next, a plurality of deletion derivatives of the cloned cDNA is prepared such that the deletions serially extend into the cDNA from one end thereof. The resulting deletion library is then treated such that cDNA is removed from the other end of each cDNA insert, thus obtaining a fragment library having fragments of a selected size. A catalytic core is then inserted into each fragment of the fragment library, resulting in the directed antisense library. An illustrative antisense gene in the hammerhead ribozyme catalytic core. Plasmids for making the antisense library, plasmids and methods for making the fragment library, and a method for identifying target sites for antisense-mediated gene inhibition are also described.
Carl Kamb - Salt Lake City UT, US Sanghee Yoo - Salt Lake City UT, US Miguel Garcia-Guzman - Salt Lake City UT, US Michael Pierce - Salt Lake City UT, US
Methods for assaying a cellular pathway, and more particularly a -catenin-related pathway, are disclosed. The assays of the invention utilize particular host cells with desired -catenin pathway elements, and results in the identification of biologically active phenotypic probes and cellular targets and fragments, variants and mimetics thereof.
In Vivo Method For Identifying Target Sites For Antisense-Mediated Inhibition Of A Selected Gene
Duane Ruffner - Salt Lake City UT, US Michael Pierce - Salt Lake City UT, US Zhidong Chen - Salt Lake City UT, US
International Classification:
C12Q001/68 G01N033/53 C12N015/85
US Classification:
435/006000, 435/007100, 435/455000
Abstract:
A method for making a directed antisense library against a target transcript is described. A cDNA of the target transcript is cloned in an appropriate cloning vector. Next, a plurality of deletion derivatives of the cloned cDNA is prepared such that the deletions serially extend into the cDNA from one end thereof. The resulting deletion library is then treated such that cDNA is removed from the other end of each cDNA insert, thus obtaining a fragment library having fragments of a selected size. An antisense gene is then inserted into each fragment of the fragment library, resulting in the directed antisense library. An illustrative antisense gene in the hammerhead ribozyme catalytic core. Plasmids for making the antisense library, plasmids and methods for making the fragment library, and a method for identifying target sites for antisense-mediated gene inhibition are also described.
In Vitro Method For Identifying Target Sites For Antisense-Mediated Inhibition Of A Selected Gene
Duane Ruffner - Salt Lake City UT, US Michael Pierce - Salt Lake City UT, US Zhidong Chen - Salt Lake City UT, US
International Classification:
C12Q001/68 G01N033/53 C12N015/85
US Classification:
435/006000, 435/007100, 435/455000
Abstract:
A method for making a directed antisense library against a target transcript is described. A cDNA of the target transcript is cloned in an appropriate cloning vector. Next, a plurality of deletion derivatives of the cloned cDNA is prepared such that the deletions serially extend into the cDNA from one end thereof. The resulting deletion library is then treated such that cDNA is removed from the other end of each cDNA insert, thus obtaining a fragment library having fragments of a selected size. An antisense gene is then inserted into each fragment of the fragment library, resulting in the directed antisense library. An illustrative antisense gene in the hammerhead ribozyme catalytic core. Plasmids for making the antisense library, plasmids and methods for making the fragment library, and a method for identifying target sites for antisense-mediated gene inhibition are also described.
Fused Pyrimidones And Thiopyrimidones, And Uses Thereof
Raj Gopal Venkat - Salt Lake City UT, US Longwu Qi - West Lafayette IN, US Michael Pierce - Salt Lake City UT, US Paul B. Robbins - Park City UT, US Sudhir R. Sahasrabudhe - Sandy UT, US Robert Selliah - Midvale UT, US
Assignee:
PROLEXYS PHARMACEUTICALS, INC. - SALT LAKE CITY UT
Mar 2011 to 2000 Sr. Process Manufacturing EngineerZeroScape Synthetic Turf Solutions
2007 to 2000 Operations ManagerOverstock.com Salt Lake City, UT 2009 to 2010 Sr. Process Improvement EngineerParker Hannifin EPS Division Salt Lake City, UT 2003 to 2007 Process EngineerParker Hannifin EPS Division
1999 to 2003 Lean Continuous Improvement ManagerBRIGHAM YOUNG UNIVERSITY Provo, UT 1996 to 1999 Electricians' AssistantWORDPERFECT CORPORATION Orem, UT 1990 to 1996 Logistics Coordinator
Education:
BRIGHAM YOUNG UNIVERSITY Provo, UT 2000 Bachelor of Science in Manufacturing Engineering Technology
Sandra Day O'Connor College of Law Natural Resources Clinic Tempe, AZ Sep 2012 to Nov 2012 Student ClinicianArizona Attorney General's Office Gilbert, AZ Jun 2012 to Aug 2012 Student ExternSandra Day O'Connor College of Law Immigration Law Clinic Tempe, AZ Mar 2012 to May 2012 Student ClinicianHBLL Security Provo, UT Jan 2008 to Aug 2010 Senior Security OfficerPolitical Science Department Provo, UT Mar 2009 to May 2009 Research AssistantDow Chemical Employees' Credit Union Midland, MI Jun 2007 to Jun 2008 Item ProcessorChurch of Jesus Christ of Latter-day Saints
May 2005 to Jun 2007 Volunteer RepresentativeDow Chemical Employees' Credit Union Midland, MI Nov 2002 to Aug 2004 Member Service RepresentativeChurch of Jesus Christ of Latter-day Saints Tempe, AZ Volunteer Religious Leader
Education:
Sandra Day O'Connor College of Law, Arizona State University Tempe, AZ Dec 2012 J.D. in LawBrigham Young University Provo, UT 2004 to 2010 BA in Political Science (Minor in Management)
Aug 2013 to 2000 Law ClerkSandra Day O'Connor College of Law Natural Resources Clinic Tempe, AZ Sep 2012 to Nov 2012 Student ClinicianArizona Attorney General's Office Gilbert, AZ Jun 2012 to Aug 2012 Student ExternSandra Day O'Connor College of Law Immigration Law Clinic Tempe, AZ Mar 2012 to May 2012 Student ClinicianHBLL Security Provo, UT 2008 to 2010 Senior Security OfficerPolitical Science Department Provo, UT Mar 2009 to May 2009 Research AssistantDow Chemical Employees' Credit Union Midland, MI 2002 to 2004 Item Processor
Education:
Sandra Day O'Connor College of Law at Arizona State University Tempe, AZ Dec 2012 J.D. in LawBrigham Young University Provo, UT Aug 2010 B.A. in Political Science
Skills:
Interpersonal Skills, Problem Solving, Customer Service, MS Office Suite, Leadership, Academics, Written Communication, Verbal Communication, Research
United States Air Force Houston, TX Feb 1992 to Feb 2013 Air Force RecuiterFairfield High School Fairfield, CA Jun 2004 to May 2006 Teacher of MathematicsRoy Middle School Roy, UT Jun 2003 to May 2004 Teacher of TechnologyUnited States Air Force Tacoma, WA Mar 1980 to Feb 1992 ElectricianUnited States Marine Corps Camp Pendleton, CA Nov 1975 to Aug 1979 Combat Engineer
Education:
Texas Southern University Houston, TX 2000 to 2002 Master - Industrial Technology in Quality Control & First Line SupervisionSouthern Illinois University, Carbondale Carbondale, IL 1984 to 1986 Bachelor of Science, Industrial Technology in Production Control & First Line SupervisionClinton Comm College Plattsburgh, NY 1983 to 1985 Associate in Science, Mathematics in General Mathematics
Salt Lake City, Utah Lindon, Utah Missouri, Washington, Florida, Illinois
Work:
Mobile/Video Marketing Experts LLC - VP Mobile Marketing
Education:
Utah Valley State College, University of Utah
About:
We build Premium Mobile sites and create SMS follow up marketing campaigns. We always keep in mind that conversions are the goal so our mobile sites are built to capture leads and convert them to clie...
Tagline:
Mobile Marketing Consultant. Text Message Marketing Campaigns, Facebook Marketing Campaigns and SMS Marketing to enhance and increase business. Online video commercials, Online media campaigns, Website Creation, Group Coupon Campaign Management, Google Places and all aspects if internet marketing.
Bragging Rights:
Mobile Marketing Consultant. Text Message Marketing Campaigns, Facebook Marketing Campaigns and SMS Marketing to enhance and increase business. Online video commercials, Online media campaigns, Website Creation, Group Coupon Campaign Management, Google Places and all aspects if internet marketing.
Michael Pierce
Education:
University of Southern California - Annenberg School of Communication and Journalism, Arizona State University
Tagline:
“Don't think about art when you're working. Try to think about life.” - Jean-Michel Basquiat
Bragging Rights:
Little League manager to 2nd place AA Clemson Tigers 2012 and award winning filmmaker and advertising guy.
Michael Pierce
Lived:
Spanish Fork, Utah, USA
Work:
Retired
Education:
Hard Knocks University
Michael Pierce
Work:
Me, myself and i - Smartass (1983)
Education:
Wright State University - International studies
Tagline:
Tag! You're it!
Michael Pierce
Education:
Harvard Medical School, Trinity College - Neuroscience, Wellesley High School
Michael Pierce
Work:
Retired - The man
Education:
North Kingstown senior high - Vocational,machinist