Michael Reidy

US Patent:

20100240597, Sep 23, 2010

Filed:

Jun 12, 2008

Appl. No.:

12/664342

Inventors:

Carole L. Cramer - Jonesboro AR, US
Maureen C. Dolan - Jonesboro AR, US
Michael J. Reidy - Rockville MD, US

Assignee:

ARKANSAS STATE UNIVERSITY - State University AR

International Classification:

A61K 38/12
C07K 14/415
C12P 21/06
C12N 5/02
C12N 5/10

US Classification:

514 212, 530370, 435 701, 435375, 435419

Abstract:

A method of preparing molecules of interest for delivery to eukaryotic cells is shown, wherein a ricin B chain subunit not having a ribosome inactivating subunit and retaining lectin activity is modified by modifying the first cysteine residue to be absent or substituted with an amino acid other than cysteine, or removing a protease sensitive site at the N-terminal of the subunit, or adding an endoplasmic reticulum retrieval signal, and operatively associating the subunit with a molecule of interest. Methods of operatively associating the subunit and molecule of interest include chemical conjugation at primary amines, conjugation with N-glycans of the subunit, a disulfide bond, and assembly of immunoglobulin domains. The invention provides for operatively associating multiple molecules of interest with a ricin B chain subunit, and delivery into targeted cells, cell components, and combination of cells.