Michael L Samuels

US Patent:

20100022414, Jan 28, 2010

Filed:

Jul 17, 2009

Appl. No.:

12/504764

Inventors:

Darren Link - Lexington MA, US
Brian Hutchison - Arlington MA, US
Michael Samuels - Windham NH, US
Michael Weiner - Guilford CT, US

Assignee:

Raindance Technologies, Inc. - Lexington MA

International Classification:

C40B 40/10
C40B 40/04
C40B 50/08

US Classification:

506 18, 506 15, 506 27

Abstract:

The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.

US Patent:

20120122714, May 17, 2012

Filed:

Sep 30, 2011

Appl. No.:

13/250702

Inventors:

Michael Samuels - Windham NH, US
Darren Roy Link - Lexington MA, US

Assignee:

RAINDANCE TECHNOLOGIES, INC. - Lexington MA

International Classification:

C40B 30/04
C40B 40/12
C40B 40/06
C40B 40/10
G01N 33/53
C40B 40/04

US Classification:

506 9, 436501, 506 15, 506 16, 506 18, 506 19

Abstract:

The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

US Patent:

20120220494, Aug 30, 2012

Filed:

Feb 16, 2012

Appl. No.:

13/398677

Inventors:

Michael Samuels - Windham NH, US
Jeffrey Charles Olson - Chelmsford MA, US
Andrew Watson - Bedford MA, US
Keith Brown - Carlsbad CA, US
Darren Roy Link - Lexington MA, US

Assignee:

RAINDANCE TECHNOLGIES, INC. - Lexington MA

International Classification:

C40B 50/08
C40B 40/06
C40B 40/10
C40B 50/06

US Classification:

506 16, 506 27, 506 26, 506 18

Abstract:

The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

US Patent:

20120264646, Oct 18, 2012

Filed:

Jun 1, 2012

Appl. No.:

13/487030

Inventors:

Darren R. Link - Lexington MA, US
Michael L. Samuels - Windham NH, US

Assignee:

Raindance Technologies, Inc. - Lexington MA

International Classification:

C40B 30/08
G01N 33/566
G01N 21/00
C12M 1/40
G01N 33/573
G01N 21/64

US Classification:

506 11, 435 74, 435 23, 435 61, 435 18, 4352887, 435 794

Abstract:

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

US Patent:

20130210659, Aug 15, 2013

Filed:

Feb 11, 2013

Appl. No.:

13/764459

Inventors:

Andrew Watson - Bedford MA, US
Darren Roy Link - Lexington MA, US
Jeffrey Charles Olson - Chelmsford MA, US
Michael Samuels - Windham NH, US

International Classification:

C12N 15/10
C12Q 1/68

US Classification:

506 9

Abstract:

The invention generally relates to method for screening for a condition in a subject. In certain embodiments, methods of the invention involve obtaining a pool of nucleic acids from a sample, incubating the nucleic acids with first and second sets of binders, in which the first set binds uniquely to different regions of a target nucleic acid in the pool, the second set binds uniquely to different regions of a reference nucleic acid in the pool, and the first and second sets include different detectable labels, removing unbound binders, detecting the labels, and screening for a condition based upon results of the detecting step.

US Patent:

20210395808, Dec 23, 2021

Filed:

Sep 3, 2021

Appl. No.:

17/466309

Inventors:

- Hercules CA, US
Michael L. Samuels - Windham NH, US

International Classification:

C12Q 1/6844
C12Q 1/6858

Abstract:

The invention generally relates to methods for distinguishing a rare genetic variation in a nucleic acid sequence.

US Patent:

20210088519, Mar 25, 2021

Filed:

Oct 27, 2020

Appl. No.:

17/081220

Inventors:

- Hercules CA, US
Michael L. Samuels - Windham NH, US

International Classification:

G01N 33/573
B01F 5/06
B01J 19/00
B01F 13/00
B01L 3/00
B01F 3/08
C40B 40/04
C40B 50/08
C40B 60/10
G01N 33/50
C12Q 1/25
C12Q 1/6804
C12Q 1/6818
C12Q 1/6827
G01N 33/542
G01N 33/58

Abstract:

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

US Patent:

20200249230, Aug 6, 2020

Filed:

Feb 3, 2020

Appl. No.:

16/780508

Inventors:

- Hercules CA, US
Michael L. Samuels - Windham NH, US

International Classification:

G01N 33/573
G01N 33/58
G01N 33/542
C12Q 1/6827
C12Q 1/6818
C12Q 1/6804
C12Q 1/25
G01N 33/50
C40B 60/10
C40B 50/08
C40B 40/04
B01F 3/08
B01L 3/00
B01F 13/00
B01J 19/00
B01F 5/06

Abstract:

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the sunset. The amount of target can be determined based on the detected number.