Replicable libraries having discrete members in defined locations for screening for antigens to a pathogenic organism are provided. Also provided are methods for using such libraries as well as a specific antigen, CT788, which induces T-cell activation during a infection.
Use Of Toxin Peptides And/Or Affinity Handles For Delivering Compounds Into Cells
R. JOHN COLLIER - WELLESLEY MA, US STEVEN R. BLANKE - HOUSTON TX, US JILL C. MILNE - BROOKLINE MA, US ERICKA L. BENSON - KALAMAZOO MI, US JIMMY D. BALLARD - NORMAN OK, US MICHAEL N. STARNBACH - NEEDHAM MA, US
A method and compositions for delivering a compound to the cytoplasm of a cell are disclosed. The compound to be delivered may be an antigenic compound, may be linked to a polycationic affinity handle, or both. In one of the methods disclosed, the B moiety of a toxin, such as the anthrax PA polypeptide, is also provided to enhance delivery of the compound to the cytoplasm of the cell.
- Cambridge MA, US - Boston MA, US Michael N. Starnbach - Needham MA, US Robert S. Langer - Newton MA, US Omid C. Farokhzad - Waban MA, US Ulrich Von Andrian - Chestnut Hill MA, US
Provided herein are new compositions including an inactivated pathogen and one or more adjuvant-loaded polymeric nanoparticles, wherein the adjuvant-loaded nanoparticles are bound to the inactivated pathogen. These compositions are useful for preventing and/or treating diseases caused by the specific pathogens, especially when administered to a subject's mucosal membranes.
Compositions For And Methods Of Identifying Antigens
Replicable libraries having discrete members in defined locations for screening for antigens to a pathogenic organism are provided. Also provided are methods for using such libraries as well as a specific antigen, CT788, which induces T-cell activation during a infection.
Targeted Cytosolic Delivery Of Antigenic Compounds
- Cambridge MA, US R. John COLLIER - Wellesley MA, US Catarina V. NOGUEIRA - Somerville MA, US Michael STARNBACH - Needham MA, US
Assignee:
President and Fellows of Harvard College - Cambridge MA
International Classification:
A61K 39/385
Abstract:
The invention relates to engineered anthrax toxin compositions that can target antigen-presenting cells such as dendritic cells. Specifically, the compositions comprise (a) a native-receptor-ablated anthrax toxin protective antigen (PA) fused to a receptor-binding moiety specific for a target receptor on a dendritic cell and (b) a lethal factor (LF) or a fragment thereof fused to an active moiety comprising at least one repeat of a disease-specific antigen. The invention also relates to methods of using these compositions for targeted delivery to dendritic cells, methods of enhancing CTL activation, and methods of inducing an immune response to cancers, bacteria, and/or viruses.
Compositions For And Methods Of Identifying Antigens
- Cambridge MA, US Michael N. Starnbach - Needham MA, US Todd Gierahn - Brookline MA, US Nadia R. Roan - San Francisco CA, US
International Classification:
C12N 15/10
Abstract:
Replicable libraries having discrete members in defined locations for screening for antigens to a pathogenic organism are provided. Also provided are methods for using such libraries as well as a specific antigen, CT788, which induces T-cell activation during a infection.
- Cambridge, MA, US - Boston MA, US Michael N. STARNBACH - Needham MA, US Robert S. LANGER - Newton MA, US Omid C. FAROKHZAD - Waban MA, US Ulrich Von ANDRIAN - Chestnut Hill MA, US
International Classification:
A61K 39/118 A61K 39/04 A61K 39/02
Abstract:
Provided herein are new compositions including an inactivated pathogen and one or more adjuvant-loaded polymeric nanoparticles, wherein the adjuvant-loaded nanoparticles are bound to the inactivated pathogen. These compositions are useful for preventing and/or treating diseases caused by the specific pathogens, especially when administered to a subject's mucosal membranes.