Michael Paul Vickery

US Patent:

20060166232, Jul 27, 2006

Filed:

Nov 15, 2005

Appl. No.:

11/280474

Inventors:

Michael Vickery - Birmingham AL, US
Angelo DePaola - Coden AL, US
George Blackstone - Theodore AL, US

International Classification:

C12Q 1/68
C07H 21/04

US Classification:

435006000, 536024100

Abstract:

The invention provides an internal control nucleic acid molecule including at least one forward primer binding site, at least one reverse primer binding site, and at least one amplifiable region, wherein the forward primer binding site, the reverse primer binding site, and the amplifiable region are all randomly generated. The invention also provides a kit that includes at least one internal control nucleic acid molecule of the invention, at least one forward primer, configured to be complementary to the forward primer binding site of the internal control nucleic acid molecule, and at least one reverse primer, configured to be complementary to the reverse primer binding site of the internal control nucleic acid molecule. The invention also provides methods of using the internal control nucleic acid molecules and kits of the invention.

US Patent:

20060110724, May 25, 2006

Filed:

Nov 19, 2004

Appl. No.:

10/994158

Inventors:

William Burkhardt - Mobile AL, US
Michael Vickery - Birmingham AL, US
Jessica Nordstrom - Irvington AL, US

International Classification:

C12Q 1/70
C12Q 1/68
C12P 19/34

US Classification:

435005000, 435006000, 435091200

Abstract:

A method is provided for reverse transcription-polymerase chain reaction (RT-PCR) comprising a) amplifying a reverse transcribed cDNA in a mixture comprising Norovirus Genogroup I and Norovirus Genogroup II primers and probes, wherein said Norovirus primers and probes distinguish between Genogroup I and Genogroup II viruses; b) quantifying virus; and c) normalizing data based on a universal internal RNA control. Optionally, the method may also include primers and probes for Enteroviruses. A reaction mixture comprising Norovirus Genogroup I and Norovirus Genogroup II primers and probes, wherein said Norovirus primers and probes distinguish between Genogroup I and Genogroup II viruses and universal internal RNA control primers and probes are also included.