Michael P Weiner

US Patent:

7244567, Jul 17, 2007

Filed:

Jan 28, 2004

Appl. No.:

10/768729

Inventors:

Yi-Ju Chen - New Haven CT, US
John H. Leamon - Guilford CT, US
Kenton L. Lohman - Guilford CT, US
Michael T. Ronan - New Haven CT, US
Jonathan M. Rothberg - Guilford CT, US
Maithreyan Srinivasan - Branford CT, US
Michael P. Weiner - Guilford CT, US

Assignee:

454 Life Sciences Corporation - Branford CT

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 911

Abstract:

This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer to a first strand of nucleic acid; annealing a second blocked primer to a second strand of nucleic acid; elongating the nucleic acid along the first strand with a polymerase; terminating the first sequencing primer; deblocking the second primer; and elongating the nucleic acid along the second strand.

US Patent:

7323305, Jan 29, 2008

Filed:

Sep 22, 2004

Appl. No.:

10/767779

Inventors:

John H. Leamon - Guilford CT, US
Kenton L. Lohman - Guilford CT, US
Jonathan M. Rothberg - Guilford CT, US
Michael P. Weiner - Guilford CT, US

Assignee:

454 Life Sciences Corporation - Branford CT

International Classification:

C12Q 1/68
C12P 19/34
C12M 1/34
C12M 1/40
C07H 21/04
C12Q 1/66
C12N 11/18

US Classification:

435 6, 435 8, 435 912, 435 9152, 435175, 4352872, 4352887, 536 242, 536 2433

Abstract:

An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.

US Patent:

7335762, Feb 26, 2008

Filed:

Aug 15, 2002

Appl. No.:

10/222592

Inventors:

Jonathan M. Rothberg - Guilford CT, US
Joel S. Bader - Stamford CT, US
Scott B. Dewell - New Haven CT, US
Keith McDade - Clinton CT, US
John W. Simpson - Madison CT, US
Jan Berka - New Haven CT, US
Christopher M. Colangelo - Old Lyme CT, US
Michael P. Weiner - Guilford CT, US

Assignee:

454 Life Sciences Corporation - Branford CT

International Classification:

C07H 21/04
C12Q 1/68
C12P 19/34

US Classification:

536 243, 435 6, 435 911, 435 912

Abstract:

Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (>10,000) of different oligonucleotides.

US Patent:

7842457, Nov 30, 2010

Filed:

Jan 28, 2004

Appl. No.:

10/767899

Inventors:

Jan Berka - New Haven CT, US
Yi-Ju Chen - New Haven CT, US
John H. Leamon - Guilford CT, US
Steve Lefkowitz - Branford CT, US
Kenton L. Lohman - Guilford CT, US
Vinod B. Makhijani - Guilford CT, US
Jonathan M. Rothberg - Guilford CT, US
Gary J. Sarkis - Guilford CT, US
Maithreyan Srinivasan - Branford CT, US
Michael P. Weiner - Guilford CT, US

Assignee:

454 Life Sciences Corporation - Branford CT

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 912

Abstract:

Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

US Patent:

8012690, Sep 6, 2011

Filed:

Oct 31, 2007

Appl. No.:

11/982095

Inventors:

Jan Berka - New Haven CT, US
Yi-Ju Chen - New Haven CT, US
John H. Leamon - Guilford CT, US
Steve Lefkowitz - Branford CT, US
Kenton L. Lohman - Guilford CT, US
Vinod B. Makhijani - Guilford CT, US
Jonathan M. Rothberg - Guilford CT, US
Gary J. Sarkis - Guilford CT, US
Maithreyan Srinivasan - Branford CT, US
Michael P. Weiner - Guilford CT, US

Assignee:

454 Life Sciences Corporation - Branford CT

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 612, 435 912

Abstract:

Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

US Patent:

8158359, Apr 17, 2012

Filed:

Jun 24, 2010

Appl. No.:

12/803362

Inventors:

John H. Leamon - Guilford CT, US
Kenton L. Lohman - Guilford CT, US
Jonathan M. Rothberg - Guilford CT, US
Michael P. Weiner - Guilford CT, US

Assignee:

454 Lice Sciences Corporation - Branford CT

International Classification:

C12Q 1/68

US Classification:

435 612

Abstract:

An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.

US Patent:

20020042137, Apr 11, 2002

Filed:

Jul 11, 2001

Appl. No.:

09/903452

Inventors:

Cynthia Richards - Durham NC, US
Michael Weiner - Cary NC, US

International Classification:

C12N015/861
C07H021/04
C12N007/01

US Classification:

435/456000, 435/235100, 536/023720

Abstract:

The present invention provides a system for simple generation of recombinant animal viruses. The system includes a virus homing vector and can further comprise a transfer vector. These components are used in a system that reduces the number of cloning steps and provides for easier preparation of a number of recombinant viruses.

US Patent:

20020142345, Oct 3, 2002

Filed:

Dec 22, 2000

Appl. No.:

09/747003

Inventors:

Anita Nelsen - Raleigh NC, US
Lottie Peppers - Apex NC, US
Michael Weiner - Cary NC, US

International Classification:

C12Q001/68
G01N033/53
G06F019/00

US Classification:

435/007100, 435/006000, 702/019000

Abstract:

The present invention provides a method of encoding a complex mixture of assay constituents comprising using combinations of detectable tags and a total number of detectable tags that is less than the total number of constituents to be encoded. More specifically, the method further comprises determining the total number of constituents to be encoded; determining the number of detectable tags in each combination, wherein the number of detectable tags in each combination is more than one and less than or equal to the number of prime numbers in the number of constituents to be encoded; and determining the total number of detectable tags, wherein the total number of detectable tags equals a sum of a set of factors of the total number of constituents, wherein the number of factors equals the number of detectable tags in each combination. The invention further provides using the encoding methods in a method of performing a multiplexed assay using complex mixtures of assay constituents and a kit for performing the multiplexed assay using complex mixtures of encoded assay constituents.