Michael R Weiner

US Patent:

20040185484, Sep 23, 2004

Filed:

Jan 28, 2004

Appl. No.:

10/767894

Inventors:

Gina Costa - Beverly MA, US
John Leamon - Guilford CT, US
Jonathan Rothberg - Guilford CT, US
Michael Weiner - Guilford CT, US

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

This invention relates to methods of generating single stranded DNA libraries for use in amplification and sequencing reactions. In various aspects, the disclosed methods include: fragmenting DNA; polishing the fragments' ends; ligating the fragments to universal adaptors; performing strand displacement and extension of the nicked fragments; purifying the double-stranded ligation products; capturing the double-stranded ligation products onto a solid support; and isolating single stranded DNA library fragments, and binding these fragments to another solid support.

US Patent:

20090011959, Jan 8, 2009

Filed:

May 27, 2008

Appl. No.:

12/154901

Inventors:

Gina L. Costa - Beverly MA, US
John H. Leamon - Guilford CT, US
Jonathan M. Rothberg - Guilford CT, US
Michael P. Weiner - Guilford CT, US

International Classification:

C40B 50/14

US Classification:

506 30

Abstract:

This invention relates to methods of generating single stranded DNA libraries for use in amplification and sequencing reactions. In various aspects, the disclosed methods include: fragmenting DNA; polishing the fragments' ends; ligating the fragments to universal adaptors; performing strand displacement and extension of the nicked fragments; purifying the double-stranded ligation products; capturing the double-stranded ligation products onto a solid support; and isolating single stranded DNA library fragments, and binding these fragments to another solid support.

US Patent:

20100022414, Jan 28, 2010

Filed:

Jul 17, 2009

Appl. No.:

12/504764

Inventors:

Darren Link - Lexington MA, US
Brian Hutchison - Arlington MA, US
Michael Samuels - Windham NH, US
Michael Weiner - Guilford CT, US

Assignee:

Raindance Technologies, Inc. - Lexington MA

International Classification:

C40B 40/10
C40B 40/04
C40B 50/08

US Classification:

506 18, 506 15, 506 27

Abstract:

The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.

US Patent:

20100137163, Jun 3, 2010

Filed:

Jun 1, 2006

Appl. No.:

12/087713

Inventors:

Darren R. Link - Lexington MA, US
Laurent Boitard - Paris, FR
Jeffrey Branciforte - Cromwell CT, US
Yves Charles - Waterbury CT, US
Gilbert Feke - Durham CT, US
John Q. Lu - Plano TX, US
David Marran - Durham CT, US
Ahmadali Tabatabai - Branford CT, US
Michael Weiner - Guilford CT, US
Wolfgang Hinz - Guilford CT, US
Jonathan M. Rothberg - Guilford CT, US

International Classification:

C40B 40/06
C40B 60/14
C40B 60/08
C40B 50/14

US Classification:

506 16, 506 40, 506 37, 506 30

Abstract:

The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.

US Patent:

20130090248, Apr 11, 2013

Filed:

Sep 27, 2012

Appl. No.:

13/629227

Inventors:

Darren R. Link - Lexington MA, US
Michael Weiner - Guilford CT, US
David Marran - Durham CT, US
Jonathan M. Rothberg - Guilford CT, US

International Classification:

C12Q 1/68

US Classification:

506 2, 506 9

Abstract:

The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

US Patent:

20130183659, Jul 18, 2013

Filed:

Feb 20, 2013

Appl. No.:

13/772002

Inventors:

Darren R. Link - Lexington MA, US
Michael Weiner - Guilford CT, US
David Marran - Durham CT, US
Jonathan M. Rothberg - Guilford CT, US

International Classification:

G01N 27/327

US Classification:

435 5, 435 612

Abstract:

The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

US Patent:

20130210639, Aug 15, 2013

Filed:

Feb 28, 2013

Appl. No.:

13/779943

Inventors:

Darren R. Link - Lexington MA, US
Michael Weiner - Guilford CT, US
David Marran - Durham CT, US
Jonathan M. Rothberg - Guilford CT, US

International Classification:

C12N 15/10
C12Q 1/68

US Classification:

506 2, 435 612, 435 912, 506 26

Abstract:

The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

US Patent:

20130217583, Aug 22, 2013

Filed:

Feb 5, 2013

Appl. No.:

13/759660

Inventors:

Darren Link - Lexington MA, US
Laurent Boitard - Gullford CT, US
Jeffrey Branciforte - Cromwell CT, US
Yves Charles - Waterbury CT, US
Gilbert Feke - West Simsbury CT, US
John Q. Lu - Gullford CT, US
David Marran - Durham CT, US
Ahmadali Tabatabai - Branford CT, US
Michael Weiner - Guilford CT, US
Wolfgang Hinz - Guilford CT, US
Jonathan M. Rothberg - Guilford CT, US

International Classification:

C12Q 1/68
G01N 33/543

US Classification:

506 2, 506 9

Abstract:

The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.