Michele M Mauro

US Patent:

53506936, Sep 27, 1994

Filed:

Apr 8, 1993

Appl. No.:

8/044400

Inventors:

Jonathan Maimon - Rego Park NY
Bruce S. Schneider - Great Neck NY
Kenneth C. Gorray - Fresh Meadows NY
Michele Mauro - Bayside NY

Assignee:

Long Island Jewish Medical Center - New Hyde Park NY

International Classification:

C12M 100
C12M 102

US Classification:

435287

Abstract:

An apparatus for fusing cells which includes a multichamber syringe having a first chamber containing a suspension of cells, a second chamber containing a suspension of cells, and a third chamber containing at least 40% by volume polyethylene glycol (PEG). The exit passageways of the chambers being braided such that the downstream ends thereof are beveled and face one another at the same level. The relative cross sections of the chambers being of a diameter such that a desired ratio of the suspensions and solution form in midair a mixture of 15% to 25% PEG by volume. The apparatus also includes a non-linear tube in fluid communication with the syringe for receiving the mixture therefrom and a device for causing a reciprocating passage of the mixture through the non-linear tube.