Michelle L Lane

US Patent:

6762053, Jul 13, 2004

Filed:

Jun 8, 2001

Appl. No.:

09/877395

Inventors:

David K. Gardner - Highlands Ranch CO
Michelle T. Lane - Highlands Ranch CO

Assignee:

Vitrolife, Inc. - Englewood CO

International Classification:

C12N 500

US Classification:

435407, 435325, 435404

Abstract:

The present invention provides a supplement and a culture media useful for culturing mammalian gametes and embryonic tissue. The culture media comprises at least one of recombinant human albumin, fermented hyaluronan, and citrate. Because the constituents are produced from non-conventional sources, the culture medium is free from contaminants such as viruses, prions and endotoxins. Additionally, because the medium is completely defined, the medium is not subject to variations which can impair the development of mammalian cells and prevent meaningful comparisons of empirical studies.

US Patent:

6838235, Jan 4, 2005

Filed:

Dec 18, 2002

Appl. No.:

10/322914

Inventors:

David K. Gardner - Highlands Ranch CO, US
Michelle Lane - Highlands Ranch CO, US

Assignee:

Vitrolife Group - Gothenburg

International Classification:

A01N 102
C12N 508

US Classification:

435 2, 435366, 435374, 435404

Abstract:

Instead of immersing human reproductive cells in a single culture medium throughout the various procedures used in IVF, a process is provided by which the reproductive cells may be moved through a sequence of distinct culture media as the various IVF procedures are carried out. In one implementation, the culture media specifically formulated to provide a physical environment similar to that found within the female reproductive tract and conducive to growth and development of human reproductive cells during the various stages of the IVF process. In this regard, specifically formulated culture media can be applied to support the reproductive cells in one or more of the following procedures: oocyte retrieval and handling; oocyte maturation; ordinary fertilization; oocyte, zygote and embryo examination and biopsy; embryonic development to the eight-cell stage; embryonic development to the blastocyst stage; embryo transfer; and cryopreservation.

US Patent:

20060292688, Dec 28, 2006

Filed:

Apr 7, 2006

Appl. No.:

11/400746

Inventors:

David Gardner - Highlands Ranch CO, US
Michelle Lane - Littleton CO, US
Peter Svalander - Hilnlycke, SE

International Classification:

C12N 5/06

US Classification:

435325000, 435404000

Abstract:

Instead of immersing human reproductive cells in a single culture medium throughout the various procedures used in IVF, a process is provided by which the reproductive cells may be moved through a sequence of distinct culture media as the various IVF procedures are carried out. In one implementation, the culture media specifically formulated to provide a physical environment similar to that found within the female reproductive tract and conducive to growth and development of human reproductive cells during the various stages of the IVF process. In this regard, specifically formulated culture media can be applied to support the reproductive cells in one or more of the following procedures: oocyte retrieval and handling; oocyte maturation; ordinary fertilization; oocyte, zygote and embryo examination and biopsy; embryonic development to the eight-cell stage; embryonic development to the blastocyst stage; embryo transfer, and cryopreservation.