Mikhail L Shapiro

US Patent:

20220346811, Nov 3, 2022

Filed:

Jul 18, 2022

Appl. No.:

17/813177

Inventors:

- Pasadena CA, US
Abhinav AGARWAL - Pasadena CA, US
Mikhail SHAPIRO - Pasadena CA, US
Azita EMAMI - Pasadena CA, US

International Classification:

A61B 17/17

Abstract:

A three dimensional magnetic sensor attached to a surgical nail is located based on an applied monotonic magnetic field gradient. Another three dimensional magnetic sensor locates a surgical drill. A display generates a real time image of the relative alignment of the surgical drill and of the surgical nail, allowing a surgeon to repair bone fractures.

US Patent:

20220290162, Sep 15, 2022

Filed:

Mar 11, 2022

Appl. No.:

17/692778

Inventors:

- Pasadena CA, US
Mikhail G. Shapiro - Pasadena CA, US
Michael S. Yao - Pasadena CA, US

International Classification:

C12N 15/63
C12N 15/90
C12N 9/22

Abstract:

Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of probiotic cells. There are provided, in some embodiments, thermal bioswitches that allow probiotic cells to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Genetic circuits capable of inducing expression of a payload upon thermal stimulation are provided. Thermally actuated probiotic cells and methods of use for the treatment of diseases or disorders are also provided.

US Patent:

20230103980, Apr 6, 2023

Filed:

Oct 3, 2022

Appl. No.:

17/937614

Inventors:

- Pasadena CA, US
Mohamad H. Abedi - Pasadena CA, US
Mikhail G. Shapiro - Pasadena CA, US
Ann Liu - Pasadena CA, US

International Classification:

C12N 15/82
G01N 33/53
B01L 7/00
C12N 15/63

Abstract:

Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of therapeutic macrophages through a combination of molecular and physical actuation. There are provided, in some embodiments, thermal bioswitches that allow macrophages to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Genetic circuits capable of inducing expression of a payload upon thermal stimulation are provided. There are provided, in some embodiments, heat-inducible macrophages and methods of using are provided.

US Patent:

20230109152, Apr 6, 2023

Filed:

Oct 4, 2022

Appl. No.:

17/937975

Inventors:

- Pasadena CA, US
Mikhail G. Shapiro - Pasadena CA, US
Anupama Lakshmanan - Seattle WA, US
Teresa Tran - Los Angeles CA, US

International Classification:

C12Q 1/6897
C12N 5/071
C12N 15/62
C12N 15/85
A61B 8/08
C07K 14/005
C07K 14/215
C07K 14/195

Abstract:

Disclosed herein include methods, compositions, and kits suitable for use in calcium imaging. There are provided, in some embodiments, Ca-sensing GvpC proteins. Disclosed herein include Ca-sensing gas vesicles (GVs) comprising Ca-sensing GvpC proteins. In some embodiments, the Ca-sensing GvpC protein is capable of undergoing a first allosteric conformational change upon the Ca-binding domain binding Ca that causes the Ca-sensing GV to change from a GV stiff state to a GV soft state. One or more of the mechanical, acoustic, surface, and magnetic properties of a Ca-sensing GV can differ between the GV soft state and the GV stiff state.

US Patent:

20230094152, Mar 30, 2023

Filed:

Sep 28, 2022

Appl. No.:

17/936286

Inventors:

- Pasadena CA, US
Mikhail G. Shapiro - Pasadena CA, US

International Classification:

C12N 15/86
C12N 15/63
A61B 8/08

Abstract:

Disclosed herein include methods, compositions, and kits suitable for use in imaging of in situ gene expression. There are provided, in some embodiments, viral vector compositions. Disclosed herein includes a single viral vector comprising one or more gas vesicle assembly (GVA) gene(s) encoding one or more GVA protein(s), and one or more gas vesicle structural (GVS) gene(s) encoding one or more GVS protein(s). The one or more GVA protein(s) and the one or more GVS protein(s) can be capable of forming gas vesicles (GVs) upon expression in a cell.

US Patent:

20230047753, Feb 16, 2023

Filed:

Jul 22, 2022

Appl. No.:

17/814384

Inventors:

- Pasadena CA, US
Hongyi Li - Pasadena CA, US
John E. Heath - Stanford CA, US
Mikhail G. Shapiro - Pasadena CA, US

International Classification:

C12N 15/861

Abstract:

Disclosed herein include adeno-associated virus (AAV) acoustic targeting peptides. An AAV comprising the AAV acoustic targeting peptide can exhibit increased transduction at site(s) of focused ultrasound blood-brain barrier opening (FUS-BBBO), increased neuronal tropism, and diminished transduction of peripheral organs. Disclosed herein include recombinant adeno-associated virus (rAAV) comprising an AAV acoustic targeting peptide disclosed herein. Also provided herein include methods of delivering an agent to one or more target brain region(s) of a subject.

US Patent:

20230016245, Jan 19, 2023

Filed:

Jul 15, 2022

Appl. No.:

17/866240

Inventors:

- Pasadena CA, US
Mikhail G. Shapiro - Pasadena CA, US

International Classification:

C12N 15/85
A61K 49/22

Abstract:

Disclosed herein include methods, compositions, and kits enabling expression of multiple proteins from a single mRNA with a predetermined stoichiometry. There are provided, in some embodiments, nucleic acid compositions comprising a promoter operably linked to a polynucleotide comprising a first nucleic acid unit encoding first unit payload protein(s) and a second nucleic acid unit encoding second unit payload protein(s). The first nucleic acid unit and the second nucleic acid unit can each comprise a 3′ engineered translation initiation site (eTIS) comprising a three-nucleotide tunable element immediately upstream of a start codon. The eTIS of each of the first nucleic acid unit and the second nucleic acid unit can be configured to achieve a predetermined stoichiometry of the first unit payload protein(s) and the second unit payload protein(s) in a cell or cell-like environment.

US Patent:

20210324389, Oct 21, 2021

Filed:

Apr 15, 2021

Appl. No.:

17/230998

Inventors:

- Pasadena CA, US
Mikhail Shapiro - Pasadena CA, US
Dan I. Piraner - Pasadena CA, US
Justin Lee - Pasadena CA, US

International Classification:

C12N 15/63
C07K 14/725

Abstract:

Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of therapeutic T-cells through a combination of molecular and physical actuation. There are provided, in some embodiments, thermal bioswitches that allow T-cells to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Also disclosed herein are T cell activity sensors. Genetic circuits capable of inducing expression of a payload upon thermal stimulation and/or immune cell stimulation are provided. There are provided, in some embodiments, thermally actuated immune cells and methods of using are provided. Oscillator circuits and methods of preventing T cell exhaustion are also disclosed.