Miles Miller

US Patent:

20100285584, Nov 11, 2010

Filed:

Nov 1, 2007

Appl. No.:

12/312197

Inventors:

Ron Weiss - Princeton NJ, US
Ihor Lemischka - Princeton NJ, US
Priscilla Purnick - Windsor NJ, US
Christoph Schaniel - New York NY, US
Miles Miller - Indianapolis IN, US
Patrick Guye - Rupperswil, CH

International Classification:

C12N 5/071
C12N 15/79

US Classification:

435377, 4353201, 435325

Abstract:

The present invention provides compositions and methods for programming mammalian cells to perform desired functions. In particular, the present invention provides compositions and methods for programming stem cells to differentiate into a desired cell type. A quorum sensing systems that regulates the expression of cell fate regulators is introduced into mammalian host cells, such as stem cells. The quorum sensing systems generally comprises vectors that express the components of a bacterial quorum sensing pathway, including proteins which catalyze the synthesis of an autoinducer and a gene encoding a regulatory partner of the autoinducer, and vectors in which genes encoding cell fate regulators are operably linked to a promoter induced by the autoinducer/regulatory partner complex. The system can also comprise vectors in which genes encoding additional cell fate regulators are operably linked to a promoter that is induced by a factor synthesized in response to a first stage of differentiation, so that a second stage of differentiation is triggered.

US Patent:

20200063158, Feb 27, 2020

Filed:

Jul 1, 2019

Appl. No.:

16/458892

Inventors:

- Princeton NJ, US
Ihor Lemischka - Princeton NJ, US
Priscilla E.M. Purnick - West Windsor NJ, US
Christoph Schaniel - New Yord City NY, US
Miles Miller - Indianapolis IN, US
Patrick Guye - Rupperswil, CH

International Classification:

C12N 15/85

Abstract:

The present invention provides compositions and methods for programming mammalian cells to perform desired functions. In particular, the present invention provides compositions and methods for programming stem cells to differentiate into a desired cell type. A quorum sensing systems that regulates the expression of cell fate regulators is introduced into mammalian host cells, such as stem cells. The quorum sensing systems generally comprises vectors that express the components of a bacterial quorum sensing pathway, including proteins which catalyze the synthesis of an autoinducer and a gene encoding a regulatory partner of the autoinducer, and vectors in which genes encoding cell fate regulators are operably linked to a promoter induced by the autoinducer/regulatory partner complex. The system can also comprise vectors in which genes encoding additional cell fate regulators are operably linked to a promoter that is induced by a factor synthesized in response to a first stage of differentiation, so that a second stage of differentiation is triggered.

US Patent:

20170044570, Feb 16, 2017

Filed:

Aug 30, 2016

Appl. No.:

15/251894

Inventors:

- Princeton NJ, US
Ihor Lemischka - Princeton NJ, US
Priscilla E. M. Purnick - West Windsor NJ, US
Christoph Schaniel - New York NY, US
Miles Miller - Indianapolis IN, US
Patrick Guye - Rupperswil, CH

International Classification:

C12N 15/85

Abstract:

The present invention provides compositions and methods for programming mammalian cells to perform desired functions. In particular, the present invention provides compositions and methods for programming stem cells to differentiate into a desired cell type. A quorum sensing systems that regulates the expression of cell fate regulators is introduced into mammalian host cells, such as stem cells. The quorum sensing systems generally comprises vectors that express the components of a bacterial quorum sensing pathway, including proteins which catalyze the synthesis of an autoinducer and a gene encoding a regulatory partner of the autoinducer, and vectors in which genes encoding cell fate regulators are operably linked to a promoter induced by the autoinducer/regulatory partner complex. The system can also comprise vectors in which genes encoding additional cell fate regulators are operably linked to a promoter that is induced by a factor synthesized in response to a first stage of differentiation, so that a second stage of differentiation is triggered.