Ming Zhan Wu

US Patent:

6379899, Apr 30, 2002

Filed:

Mar 13, 2001

Appl. No.:

09/805674

Inventors:

Edwin Ullman - Atherton CA
Ming Wu - Castro Valley CA

Assignee:

Discoverx - Fremont CA

International Classification:

C12Q 168

US Classification:

435 6, 435 912

Abstract:

Methods and compositions are provided for performing isothermal amplification of a nucleic acid target employing probes characterized by having a masked RNA polymer promoter unable to bind to a complementary initiator oligonucleotide and RNA polymerase and initiate transcription, a dsDNA sequence which when invaded by the target nucleic acid exposes the masked promoter to initiate transcription, and a template sequence, a portion of which is normally included in the dsDNA region, which when copied produces a product that can reinitiate the process of invading the dsDNA region and initiating transcription of another copy.

US Patent:

7612355, Nov 3, 2009

Filed:

Apr 12, 2005

Appl. No.:

11/105304

Inventors:

Ming Chiang Wu - Orinda CA, US
Pei Yu Chiou - Los Angeles CA, US
Aaron T. Ohta - Berkeley CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

G01N 21/86
G01V 8/00

US Classification:

25055904, 204547, 204603

Abstract:

An optical image-driven light induced dielectrophoresis (DEP) apparatus and method are described which provide for the manipulation of particles or cells with a diameter on the order of 100 μm or less. The apparatus is referred to as optoelectric tweezers (OET) and provides a number of advantages over conventional optical tweezers, in particular the ability to perform operations in parallel and over a large area without damage to living cells. The OET device generally comprises a planar liquid-filled structure having one or more portions which are photoconductive to convert incoming light to a change in the electric field pattern. The light patterns are dynamically generated to provide a number of manipulation structures that can manipulate single particles and cells or groups of particles/cells. The OET preferably includes a microscopic imaging means to provide feedback for the optical manipulation, such as detecting position and characteristics wherein the light patterns are modulated accordingly.

US Patent:

8063196, Nov 22, 2011

Filed:

Feb 1, 2005

Appl. No.:

11/049465

Inventors:

Minxue Zheng - Foster City CA, US
David Ahle - Oakland CA, US
Brian Warner - Martinez CA, US
Ming Wu - Redwood City CA, US

Assignee:

Siemens Healthcare Diagnostics Inc. - Tarrytown NY

International Classification:

C07H 21/04
C12Q 1/68

US Classification:

536 243, 435 61

Abstract:

The invention provides a set of highly orthogonal six-code universal sequences for use in bDNA singleplex and multiplex nucleic acid hybridization assays. The six-code orthogonal sequences do not cross-hybridize and thus, minimize or eliminate the 3-mer cross-hybridization inherent in the second and third generation bDNA assays. The highly orthogonal universal sequences may be used in singleplex or multiplex bDNA assays quantitatively and qualitatively to determine mRNA levels in a sample; to screen for and genotype targets, such as viruses, that are present in low volumes in a sample; to screen for and genotype SNPs; and to measure changes in the amount of a gene in a sample such as when gene amplifications or deletions occur. The highly orthogonal universal sequences may also be used as universal capture probes to selectively bind assay components in a way that facilitates their further analysis.

US Patent:

RE44711, Jan 21, 2014

Filed:

Nov 1, 2011

Appl. No.:

13/287093

Inventors:

Ming Chiang Wu - Moraga CA, US
Pei-Yu Chiou - Los Angeles CA, US
Aaron T. Ohta - Honolulu HI, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

G01N 21/86
G01V 8/00

US Classification:

25055904, 204547, 204603

Abstract:

An optical image-driven light induced dielectrophoresis (DEP) apparatus and method are described which provide for the manipulation of particles or cells with a diameter on the order of 100 μm or less. The apparatus is referred to as optoelectric tweezers (OET) and provides a number of advantages over conventional optical tweezers, in particular the ability to perform operations in parallel and over a large area without damage to living cells. The OET device generally comprises a planar liquid-filled structure having one or more portions which are photoconductive to convert incoming light to a change in the electric field pattern. The light patterns are dynamically generated to provide a number of manipulation structures that can manipulate single particles and cells or group of particles/cells. The OET preferably includes a microscopic imaging means to provide feedback for the optical manipulation, such as detecting position and characteristics wherein the light patterns are modulated accordingly.

US Patent:

20030152924, Aug 14, 2003

Filed:

Dec 7, 2001

Appl. No.:

10/012742

Inventors:

Edwin Ullman - Atherton CA, US
Ming Wu - Castro Valley CA, US

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

Methods are provided for the determination of specific nucleotide sequences, particularly single nucleotide polymorphisms, by using probes comprising a first strand complementary to the target sequence, a shorter second strand forming a stem and a linker adjacent the double stranded stem connecting the two strands, whereby binding of target to the probe under conditions that do not cause melting of the double stranded stem in the absence of target results in the dissociation of the second strand from the first strand. The ss second strand is then detected as exemplified by using a FRET pair, where dissociation of the stem results in separation of the FRET pair and increase in fluorescence. Amplification of the target sequence may be employed prior to combination with the probe. The method finds particular application with complex nucleic acid mixtures.

US Patent:

20030165917, Sep 4, 2003

Filed:

Aug 14, 2002

Appl. No.:

10/219195

Inventors:

Edwin Ullman - Atherton CA, US
Ming Wu - Castro Valley CA, US
Yen Liu - Cupertino CA, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

A method for detection of nucleic acid targets using as reagents: (1) a stem loop probe having a long strand for hybridizing to a target, a short strand, usually with a free 3′-end for extension, hybridized to a portion of the long strand and a linker forming a loop and joining the short and long strands; and (2) a hybridizing reagent that hybridizes to the short strand when released by the target. The target is detected by extension of one or both the probe or hybridizing reagent along each other. A circular hybridizing reagent can be employed with DNA polymerase for concatenated extension of the probe 3′-end or extension of the 3′-end of the probe along a hybridizing reagent having a promoter sequence for forming transcripts of at least a portion of the long strand or the hybridizing reagent. A non-cleavable restriction site consensus sequence in the linker, where the hybridizing reagent is extended with dNTPs and DNA polymerase and the extended sequence is cleaved with a restriction enzyme, so that chains may be repetitively produced and cleaved. The presence of the target nucleic acid is established by detecting the concatenated chain, the RNA transcripts or the repetitively produced chains.

US Patent:

20030180776, Sep 25, 2003

Filed:

Feb 20, 2003

Appl. No.:

10/371600

Inventors:

Ming Wu - Castro Valley CA, US
Edwin Ullman - Atherton CA, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

Methods and compositions are provided for amplifying tandem repeats, particularly for detecting binding events, using a nucleic acid reagent comprising two features, one having a tandem repeat region and the other having an extendable 3′ terminus. When the two oligonucleotides are hybridized in the presence of a DNA polymerase and NTPs for replicating the tandem repeat region, repetitive extension of the extendable 3′ terminus along the tandem repeat region is obtained. By having labeled NTPs, the amplification can be detected as indicative of the binding event. Kits are provided for performing the method.

US Patent:

20080277356, Nov 13, 2008

Filed:

May 7, 2007

Appl. No.:

11/745411

Inventors:

Stephane Mouradian - Menlo Park CA, US
Josh Molho - Oakland CA, US
Ming Wu - Redwood City CA, US
Hayley Wu - Sunnyvale CA, US
Javier A. Farinas - Los Altos CA, US

Assignee:

Caliper Life Sciences, Inc. - Mountain View CA

International Classification:

B01D 37/00
B01L 11/00

US Classification:

210779, 210767, 422101

Abstract:

A microfluidic device with a filter includes a substrate; a flowpath including a well formed in the substrate in fluid communication with a channel formed in the substrate; and a filter disposed across the flowpath.