Disclosed are isolated nucleic acid molecules (inducible promoters) capable of directing expression in plant cell. In some embodiments, the promoters include a nucleic acid sequence set forth as SEQ ID NO: 1 or SEQ ID NO: 2, or a variant thereof. In some embodiments, the promoter is capable of gene expression in plants of an operably linked nucleic acid sequence. Disclosed are transgenic plants or parts thereof, that can include expression cassettes and/or expression vectors, which can be stably incorporated into the genome of the plant. Methods of regulating the expression of a polynucleotide of interest in a plant cell are also disclosed.
- KNOXVILLE TN, US Aditi RAMBANI - KNOXVILLE TN, US Mitra MAZAREI - KNOXVILLE TN, US Vincent PANTALONE - KNOXVILLE TN, US
International Classification:
C12N 15/82 A01H 1/04 C12Q 1/68
Abstract:
The invention relates to genes which may be utilized for resistance to soybean cyst nematode. More specifically the present disclosure relates to identification of gene(s) that can confer upon a soybean plant resistance to soybean cyst nematode (SCN) and methods to use these loci and genes to obtain soybean strains that are resistant to SCN.
Methods Of Plant Transformation Using Transformable Cell Suspension Culture And Uses Thereof
- KNOXVILLE TN, US JONATHAN D. WILLIS - KNOXVILLE TN, US JASON BURRIS - KNOXVILLE TN, US MITRA MAZAREI - KNOXVILLE TN, US
International Classification:
C12N 15/82 A01H 4/00 C12N 5/00 C12Q 1/68
Abstract:
The subject invention pertains to a method of rapidly transforming a plant with a gene of interest. The method of the current invention comprises the steps of a) preparing a bacterial culture, wherein the bacterial culture comprises a vector containing the gene of interest, b) producing a plant cell suspension culture from the plant, c) contacting the plant cell suspension culture with the bacterial culture to produce a plant cell transformed with the gene of interest from the plant cell suspension culture, and e) producing a plant from the plant cell transformed with the gene of interest. The method of the current invention can be designed for a high throughput transformation and screening of a plant with a plurality of genes of interest and screening the plants to identify and obtain plants having desirable characteristics.
Disclosed are pest resistance genes isolated from soybean. In several embodiments, a pest resistance gene isolated from soybean includes a nucleic acid sequence at least 80% identical to SEQ ID NO: 1 or SEQ ID NO: 2 or a degenerate variant thereof, or a fragment thereof. Also disclosed are expression vectors and constructs that include such nucleic acids. Methods are disclosed of producing a transgenic plant that has enhanced resistance to pests. Transgenic plants, plant cells or tissue (such as a dicotyledon or a monocotyledon plants, plant cells or tissue) transformed with the disclosed constructs are also disclosed.