Mohan S Saini

US Patent:

55433111, Aug 6, 1996

Filed:

Aug 7, 1992

Appl. No.:

7/926203

Inventors:

Barbara A. Brummond - Rochester NY
Mohan S. Saini - Pittsford NY
Ignazio S. Ponticello - Pittsford NY

Assignee:

Johnson & Johnson Clinical Diagnostics, Inc. - Rochester NY

International Classification:

C12N 996

US Classification:

435188

Abstract:

A labeled carbamazepine analogue is described comprising: (A) a label, of the type used in immunoassays, having an amine or sulfhydryl group such as horseradish peroxidase; (B) a carbamazepine nucleus; and (C) a linking chain linking the carboxamide group of the carbamazepine nucleus to the label, said linking chain having about 4 to about 40 atoms consisting of: (1) C. sub. 2 to C. sub. 6 alkylene groups; and (2) 5 to 7 membered heterocyclic ring groups, each group being joined into the linking group through chemical groups selected from (a) esters, (b) amides (c) heteroatoms selected from --O--, --S--, and --NR--; wherein R represents hydrogen or C. sub. 1 to C. sub. 6 alkyl; and (d) carbonyl. The new labeled analogues are useful in immunoassay elements and processes for detection of carbamazepine drugs, for example, in body fluids.

US Patent:

55784577, Nov 26, 1996

Filed:

Aug 7, 1992

Appl. No.:

7/926202

Inventors:

Barbara A. Brummond - Rochester NY
Mohan S. Saini - Pittsford NY
Ignazio S. Ponticello - Pittsford NY

Assignee:

Johnson & Johnson Clinical Diagnostics, Inc. - Rochester NY

International Classification:

G01N 33545
C12N 996

US Classification:

435 793

Abstract:

An immunoassay method and analytical elements for detecting carbamazepine drugs, for example, in body fluids, is described. The immunoassay method comprises: (A) contacting a liquid sample, containing carbamazepine, with a labeled carbamazepine analogue in the presence of antibodies for carbamazepine under conditions that promote the formation of carbamazepine/antibody immunocomplexes; and (B) determining the quantity of the drug in the liquid by measuring bound or unbound labeled drug analogue; characterized in that the labeled carbamazepine comprises: (1) a label, of the type used in immunoassays, having an amine or sulfhydryl group; (2) a carbamazepine nucleus; and (3) a linking chain linking the carboxamide group of the carbamazepine nucleus to the label, said linking chain having about 4 to about 40 atoms consisting of: (a) alkylene groups; and (b) heterocyclic ring groups, each group being joined into the linking group through chemical groups selected from (a) esters, (b) amides, (c) heteroatoms selected from --O--, --S--, and --NR--; wherein each R independently represents hydrogen or C. sub. 1 to C. sub. 6 alkyl; and (d) carbonyl groups. The elements of the invention are preferably dry, thin film analytical elements comprising a labeled carbamazepine analogue as described.

US Patent:

59812060, Nov 9, 1999

Filed:

May 20, 1992

Appl. No.:

7/886263

Inventors:

Thomas Charles Arter - Rochester NY
Mohan S Saini - Pittsford NY

Assignee:

Johnson & Johnson Clinical Diagnostic Systems, Inc. - Rochester NY

International Classification:

C12Q 142
C12Q 100

US Classification:

435 21

Abstract:

A dry analytical element has been prepared for the assay of prostatic acid phosphatase at a pH of from about 3 to about 6. 5. The element can be a single porous spreading zone or a multilayer structure. Within the element is a relatively nonhygroscopic aromatic phosphate substrate for the analyte which produces a phenol reaction product. This product is reacted with a diazonium or tetrazolium salt, also in the element, to produce a chromophore for detection. A buffer in the element maintains it at the proper pH during the assay.

US Patent:

53959337, Mar 7, 1995

Filed:

Aug 7, 1992

Appl. No.:

7/926205

Inventors:

Ignazio S. Ponticello - Pittsford NY
Mohan S. Saini - Pittsford NY

Assignee:

Eastman Kodak Company - Rochester NY

International Classification:

C07D22322

US Classification:

540589

Abstract:

New carbamazepine hapten analogues are described comprising: (A) an active ester group; (B) a carbamazepine nucleus; and (C) a linking chain (i) linking the carboxamide group of the carbamazepine nucleus to the active ester group, said linking chain having about 4 to about 40 atoms consisting of: (1) alkylene groups; and (2) 5 to 7 membered heterocyclic ring groups, each group being joined into the linking group through chemical groups selected from (a) esters, (b) amides, (c) hetero atoms selected from --0--, --S--, and --NR--; wherein R represents hydrogen or C. sub. 1 to C. sub. 6 alkyl; and (d) carbonyl groups. The carbamazepine-active ester analogues are useful in preparing labeled carbamazepines. The labeled carbamazepines are useful in immunoassay elements and processes for the detection of carbamazepine drugs, for example, in body fluids.