Genomics Institute of the Novartis Research Foundation San Diego, CA 2011 to 2013 Postdoctoral fellowHoward Hughes Medical Institute New York, NY 2010 to 2011 Postdoctoral fellowUC San Diego San Diego, CA 2005 to 2010 Research Associate and Teaching Assistant, University of California at San DiegoZhejiang University
2002 to 2005 Research AssociateZhejiang University
2001 to 2002 Research Assistant
Education:
University of California at San Diego San Diego, CA 2005 to 2010 PhD in BiologyZhejiang University 2002 to 2005 Master in Cell BiologyZhejiang University 1998 to 2002 Bachelor in Biology
Skills:
Cell Biology Techniques: High throughput screens and automation handling Mammalian tissue culture including human mesenchymal stem cells, hematopoietic stem cells, fibroblasts, subcutaneous preadipocytes, endothelial cells, and macrophages etc. Immunofluorescence and microscopy Cell separation using Miltenyi magnetic beads Cell-based assays including proliferation, cytotoxicity and apoptosis assays Flow cytometry Stable cell line establishment Cell-based luciferase assays In vitro target validation of small molecules and proteins Molecular Biology Techniques: ELISA SDS-PAGE and Western blot DNA/RNA extraction and RT-qPCR Microarray analysis Retrovirus production Protein expression and purification using bacteria and mammalian cells Lentivirus shRNA and siRNA knockdown Plasmid constructions In vivo Techniques: Histology and immunohistochemistry Xenograft tumor models and breast cancer models Collection and analysis of animal blood, serum and tissues Bone marrow reconstitution Intraperitoneal, subcutaneous and intravenous injections Development of animal models