Nancy Chaltain Craig

US Patent:

20020188105, Dec 12, 2002

Filed:

Dec 19, 2001

Appl. No.:

10/024809

Inventors:

Nancy Craig - Baltimore MD, US

Assignee:

Johns Hopkins University School of Medicine

International Classification:

C12N009/16
C12N015/74
C12P021/02
C07K014/705

US Classification:

530/350000, 435/196000, 435/320100, 435/473000, 435/069100

Abstract:

The invention is specifically directed to efficient, random, simple insertion of a transposon or derivative transposable element into DNA in vivo or in vitro. The invention is particularly directed to mutations in ATP-utilizing regulatory transposition proteins that permit insertion with less target-site specificity than wild-type. The invention encompasses gain-of-function mutations in TnsC, an ATP-utilizing regulatory transposition protein that activates the bacterial transposon Tn7. Such mutations enable the insertion of a Tn7 transposon or derivative transposable element in a non-specific manner into a given DNA segment. Insertion can be effected in plasmid and cosmid libraries, cDNA libraries, PCR products, bacterial artificial chromosomes, yeast artificial chromosomes, mammalian artificial chromosomes, genomic DNAs, and the like. Such insertion is useful in DNA sequencing methods, for genetic analysis by insertional mutagenesis, and alteration of gene expression by insertion of a given genetic sequence.

US Patent:

20110311506, Dec 22, 2011

Filed:

Feb 25, 2010

Appl. No.:

13/203393

Inventors:

Nancy Lynn Craig - Baltimore MD, US

Assignee:

THE JOHNS HOPKINS UNIVERSITY - Baltimore MD

International Classification:

A61K 38/45
C12N 15/63
C12N 5/10
C07H 21/04
C12N 9/12

US Classification:

424 945, 536 231, 435194, 435325, 435455

Abstract:

The present invention provides hyperactive piggyBac transposons, in particular hyperactive piggyBac transposons from (cabbage looper moth) that transpose at a higher frequency than wildtype. The invention also features integration defective piggyBac transposons. The piggyBac transposons and transposases can be used in gene transfer systems for stably introducing nucleic acids into the DNA of a cell. The gene transfer system can be used in methods, for example, but not limited to, gene therapy, insertional mutagenesis, or gene discovery.

US Patent:

20140065124, Mar 6, 2014

Filed:

Sep 26, 2013

Appl. No.:

14/038132

Inventors:

Nancy Lynn Craig - Baltimore MD, US

Assignee:

THE JOHNS HOPKINS UNIVERSITY - Baltimore MD

International Classification:

C12N 15/85

US Classification:

424 945, 536 231, 435194, 435325, 435455

Abstract:

The present invention provides hyperactive piggyBac transposons, in particular hyperactive piggyBac transposons from (cabbage looper moth) that transpose at a higher frequency than wildtype. The invention also features integration defective piggyBac transposons. The piggyBac transposons and transposases can be used in gene transfer systems for stably introducing nucleic acids into the DNA of a cell. The gene transfer system can be used in methods, for example, but not limited to, gene therapy, insertional mutagenesis, or gene discovery.

US Patent:

6420524, Jul 16, 2002

Filed:

Feb 20, 1998

Appl. No.:

09/027169

Inventors:

Nancy L. Craig - Baltimore MD

Assignee:

Johns Hopkins University School of Medicine - Baltimore MD

International Classification:

C07K 100

US Classification:

530350, 536 232

Abstract:

The invention is specifically directed to efficient, random, simple insertion of a transposon or derivative transposable element into DNA in vivo or in vitro. The invention is particularly directed to mutations in ATP-utilizing regulatory transposition proteins that permit insertion with less target-site specificity than wild-type. The invention encompasses gain-of-function mutations in TnsC, an ATP-utilizing regulatory transposition protein that activates the bacterial transposon Tn. Such mutations enable the insertion of a Tn transposon or derivative transposable element in a non-specific manner into a given DNA segment. Insertion can be effected in plasmid and cosmid libraries, cDNA libraries, PCR products, bacterial artificial chromosomes, yeast artificial chromosomes, mammalian artificial chromosomes, genomic DNAs, and the like. Such insertion is useful in DNA sequencing methods, for genetic analysis by insertional mutagenesis, and alteration of gene expression by insertion of a given genetic sequence.

US Patent:

20180072999, Mar 15, 2018

Filed:

Sep 7, 2017

Appl. No.:

15/698060

Inventors:

- Baltimore MD, US
Nancy L. Craig - Baltimore MD, US

Assignee:

The Johns Hopkins University - Baltimore MD

International Classification:

C12N 9/12
C12N 9/22
C12N 15/85
C12N 15/81
C12N 15/79
C12N 15/90

Abstract:

The present invention is directed to nucleic acid and amino acid sequences of a novel piggyBac transposase enzymes created by modifying the transposase of Trichoplusia ni. The piggyBac transposases of the present invention are functionally active or hyperactive for excision and have decreased integration activity compared to wild type Trichoplusia ni piggyBac transposase enzyme. These transposases are ideal for use in methods of transforming cells and organisms. In particular embodiments, the present invention provides methods of transient integration and expression of transgenes.

US Patent:

20170226531, Aug 10, 2017

Filed:

Apr 25, 2017

Appl. No.:

15/496286

Inventors:

- Baltimore MD, US
Nancy L. Craig - Baltimore MD, US

International Classification:

C12N 15/90
A61K 38/45
C12N 9/12

Abstract:

The present invention provides hyperactive piggyBac transposons, in particular hyperactive piggyBac transposons from (cabbage looper moth) that transpose at a higher frequency than wildtype. The invention also features integration defective piggyBac transposons. The piggyBac transposons and transposases can be used in gene transfer systems for stably introducing nucleic acids into the DNA of a cell. The gene transfer system can be used in methods, for example, but not limited to, gene therapy, insertional mutagenesis, or gene discovery.

US Patent:

20140287513, Sep 25, 2014

Filed:

Jul 16, 2012

Appl. No.:

14/233024

Inventors:

Nancy L. Craig - Baltimore MD, US

Assignee:

HOWARD HUGUES MEDICAL INSTITUTE - Chevy Chase MD
THE JOHNS HOPKINS UNIVERSITY - Baltimore MD

International Classification:

C12N 9/12
C12N 15/90

US Classification:

435462, 435194

Abstract:

The present invention is directed to nucleic acid and amino acid sequences of a novel piggyBac transposase enzymes created by modifying the transposase of The piggyBac transposases of the present invention are functionally active or hyperactive for excision and have decreased integration activity compared to wild type piggyBac transposase enzyme. These transposases are ideal for use in methods of transforming cells and organisms. In particular embodiments, the present invention provides methods of transient integration and expression of transgenes.