The invention is specifically directed to efficient, random, simple insertion of a transposon or derivative transposable element into DNA in vivo or in vitro. The invention is particularly directed to mutations in ATP-utilizing regulatory transposition proteins that permit insertion with less target-site specificity than wild-type. The invention encompasses gain-of-function mutations in TnsC, an ATP-utilizing regulatory transposition protein that activates the bacterial transposon Tn7. Such mutations enable the insertion of a Tn7 transposon or derivative transposable element in a non-specific manner into a given DNA segment. Insertion can be effected in plasmid and cosmid libraries, cDNA libraries, PCR products, bacterial artificial chromosomes, yeast artificial chromosomes, mammalian artificial chromosomes, genomic DNAs, and the like. Such insertion is useful in DNA sequencing methods, for genetic analysis by insertional mutagenesis, and alteration of gene expression by insertion of a given genetic sequence.
The present invention provides hyperactive piggyBac transposons, in particular hyperactive piggyBac transposons from (cabbage looper moth) that transpose at a higher frequency than wildtype. The invention also features integration defective piggyBac transposons. The piggyBac transposons and transposases can be used in gene transfer systems for stably introducing nucleic acids into the DNA of a cell. The gene transfer system can be used in methods, for example, but not limited to, gene therapy, insertional mutagenesis, or gene discovery.
The present invention provides hyperactive piggyBac transposons, in particular hyperactive piggyBac transposons from (cabbage looper moth) that transpose at a higher frequency than wildtype. The invention also features integration defective piggyBac transposons. The piggyBac transposons and transposases can be used in gene transfer systems for stably introducing nucleic acids into the DNA of a cell. The gene transfer system can be used in methods, for example, but not limited to, gene therapy, insertional mutagenesis, or gene discovery.
Gain Of Function Mutations In Atp-Dependent Transposition Proteins
Johns Hopkins University School of Medicine - Baltimore MD
International Classification:
C07K 100
US Classification:
530350, 536 232
Abstract:
The invention is specifically directed to efficient, random, simple insertion of a transposon or derivative transposable element into DNA in vivo or in vitro. The invention is particularly directed to mutations in ATP-utilizing regulatory transposition proteins that permit insertion with less target-site specificity than wild-type. The invention encompasses gain-of-function mutations in TnsC, an ATP-utilizing regulatory transposition protein that activates the bacterial transposon Tn. Such mutations enable the insertion of a Tn transposon or derivative transposable element in a non-specific manner into a given DNA segment. Insertion can be effected in plasmid and cosmid libraries, cDNA libraries, PCR products, bacterial artificial chromosomes, yeast artificial chromosomes, mammalian artificial chromosomes, genomic DNAs, and the like. Such insertion is useful in DNA sequencing methods, for genetic analysis by insertional mutagenesis, and alteration of gene expression by insertion of a given genetic sequence.
Trichoplusia Ni Piggybac Transposases With Reduced Integration Activity
The present invention is directed to nucleic acid and amino acid sequences of a novel piggyBac transposase enzymes created by modifying the transposase of Trichoplusia ni. The piggyBac transposases of the present invention are functionally active or hyperactive for excision and have decreased integration activity compared to wild type Trichoplusia ni piggyBac transposase enzyme. These transposases are ideal for use in methods of transforming cells and organisms. In particular embodiments, the present invention provides methods of transient integration and expression of transgenes.
- Baltimore MD, US Nancy L. Craig - Baltimore MD, US
International Classification:
C12N 15/90 A61K 38/45 C12N 9/12
Abstract:
The present invention provides hyperactive piggyBac transposons, in particular hyperactive piggyBac transposons from (cabbage looper moth) that transpose at a higher frequency than wildtype. The invention also features integration defective piggyBac transposons. The piggyBac transposons and transposases can be used in gene transfer systems for stably introducing nucleic acids into the DNA of a cell. The gene transfer system can be used in methods, for example, but not limited to, gene therapy, insertional mutagenesis, or gene discovery.
Trichoplusia Ni Piggybac Transposases With Reduced Integration Activity
HOWARD HUGUES MEDICAL INSTITUTE - Chevy Chase MD THE JOHNS HOPKINS UNIVERSITY - Baltimore MD
International Classification:
C12N 9/12 C12N 15/90
US Classification:
435462, 435194
Abstract:
The present invention is directed to nucleic acid and amino acid sequences of a novel piggyBac transposase enzymes created by modifying the transposase of The piggyBac transposases of the present invention are functionally active or hyperactive for excision and have decreased integration activity compared to wild type piggyBac transposase enzyme. These transposases are ideal for use in methods of transforming cells and organisms. In particular embodiments, the present invention provides methods of transient integration and expression of transgenes.
Jul 2011 to 2000 Associate Visiting Professor of Clinical Law and Friedman FellowEmployment Justice Center
2011 to 2000 Advising Attorney (Volunteer)Crowell & Moring, LLP Washington, DC Sep 2007 to Apr 2010 AssociateSuperior Court of the District of Columbia Washington, DC Sep 2006 to Aug 2007 Law Clerk for The Honorable Judge Brook HedgeD.C. Public Defender's Service Washington, DC Jun 2005 to Aug 2005 Law ClerkWomen Empowered Against Violence (WEAVE) Washington, DC Jan 2005 to May 2005 Legal InternD.C. Office of Human Rights Washington, DC Jun 2004 to Aug 2004 Legal InternStreet Law, Inc. Washington, DC Nov 2001 to May 2003 International Programs Assistant
Education:
George Washington University Law School Washington, DC 2011 to 2013 LLM expected Summer 2013 in lawThe George Washington University Law School Washington, DC 2003 to 2006 JD in law with highest honors and order of the coifSwarthmore College Swarthmore, PA 1997 to 2001 BA in History
Beverly, MA Raymond, NH North Hampton, NH Salem, MA Horrible campground in NH
Work:
Flair Cleaners - "all around" (1969-1996) Some security company - Security guard (1996-2000)
Education:
Beverly High School - Business
Relationship:
Its_complicated
About:
I love my sons, family, animals (especially my cat, Layla), cooking, surfing (the 'net), and want world peace for all...including animals!
Bragging Rights:
Worked full time while raising 3 sons as a single parent.
Nancy Craig
Lived:
Salem, MA
Work:
Mainly, Flair Cleaners in Beverly, MA - "All Around"
Education:
BHS - Business
About:
I'm the Nancy whom everyone *seems* to love, due to my tolerance, compassion, love, understanding and a pretty good sense of humor! I love animals and cooking (trying to get away from cooking anim...
Bragging Rights:
Funny, I DO have the best 3 sons in the WORLD! All grown up and awesome. I'm truly blessed!