Nancy H Green

US Patent:

53645910, Nov 15, 1994

Filed:

Jun 1, 1992

Appl. No.:

7/891517

Inventors:

Nancy F. Green - Pittsford NY
Thomas J. Cummins - Rochester NY
Fred T. Oakes - Melvern PA

Assignee:

Eastman Kodak Company - Rochester NY

International Classification:

G01N 33537
G01N 33546

US Classification:

422 58

Abstract:

There are described a device and method for doing confined reactions such as PCR amplification and detection, wherein solids (e. g. , beads) used to obtain separation between bound and "free" label reagents, are transferred from region to region, specifically through a wash liquid so as to wash off the "free" label reagent from the solids. Separate chambers have dividers that are overcome by piercing or by liquification, to create passageways for the transfer of the solids. The passageways remain contained within the device.

US Patent:

51550210, Oct 13, 1992

Filed:

Feb 9, 1989

Appl. No.:

7/308843

Inventors:

Richard C. Sutton - Rochester NY
Thomas J. Cummins - Rochester NY
Nancy F. Green - Pittsford NY

Assignee:

Eastman Kodak Company - Rochester NY

International Classification:

G01N 33571
G01N 33546

US Classification:

435 5

Abstract:

Herpes simplex viral antigen can be readily determined by contacting a specimen containing Herpes simplex virus of herpes simplex viral-infected cells with polymeric particles which have a surface area of from about 0. 1 to about 600 m. sup. 2 /g. Within a few minutes of this contact, antigen which is bound to the particles is contacted with antibodies thereto so as to form an immunological complex on the particles. Bound complex is separated from uncomplexed materials, and the presence of the complex is then appropriately determined. A kit for determining herpes comprises the particles described above, a disposable test device having a microporous membrane and antibodies to herpes simplex viral antigen.

US Patent:

50810103, Jan 14, 1992

Filed:

Feb 9, 1989

Appl. No.:

7/308841

Inventors:

Thomas J. Cummins - Rochester NY
Sheryl S. Sullivan - Hilton NY
Randall D. Madsen - Pleasant Hill CA
Nancy F. Green - Pittsford NY

Assignee:

Eastman Kodak Company - Rochester NY
Cetus Corporation - Emeryville CA

International Classification:

G01N 33571

US Classification:

435 5

Abstract:

An extraction composition has been found useful for extracting antigen from herpes simplex virus. This composition has a pH of from about 8. 5 to about 12, and comprises an alcoholamine or salt thereof, a nonionic surfactant comprised of a condensation product of an alkylphenol and ethylene oxide, cholic acid or a salt or derivative thereof and an anionic surfactant. Extraction of antigen is accomplished by contacting the extraction composition with a specimen suspected of containing herpes organisms under suitable conditions. Extracted antigen can be determined by forming an immunological complex with antibodies thereto and by detecting that complex. The extraction composition can be supplied as part of a diagnostic test kit.