Nancy M Hannett

US Patent:

47693274, Sep 6, 1988

Filed:

Mar 28, 1986

Appl. No.:

6/845864

Inventors:

Michael A. Stephens - Bedford MA
Cathy F. Rudolph - Canton MA
Nancy M. Hannett - Medford MA
Diane L. Stassi - Belmont MA
Janice G. Pero - Lexington MA

Assignee:

Biotechnica International, Inc. - Cambridge MA

International Classification:

C12P 2100
C12N 1500
C12N 120
C12N 100

US Classification:

435 68

Abstract:

A vector including a DNA sequence encoding a secretory signal sequence substantially identical to the secretory signal encoding sequence of the Bacillus licheniformis. alpha. -amylase gene; upstream from the signal-encoding sequence, a promoter sequence and a ribosome binding site sequence, transcription of the signal-encoding sequence being under the control of the promoter sequence; and downstream from the signal-encoding sequence, a site for the insertion into the vector of a heterologous DNA sequence, in reading frame with the signal-encoding sequence.

US Patent:

51716739, Dec 15, 1992

Filed:

Jul 18, 1988

Appl. No.:

7/219599

Inventors:

Alan Sloma - Watertown MA
Nancy M. Hannett - Medford MA
M. A. Stephens - Concord MA
Cathy F. Rudolph - Stoughton MA
Gerald A. Rufo - Burlington MA
Janice Pero - Lexington MA

Assignee:

Biotechnica International, Inc. - Cambridge MA

International Classification:

C12N 928
C12N 121
C12N 1575
C12N 2100

US Classification:

435 691

Abstract:

A vector for high-level expression of a heterologous gene in Bacillus, including a DNA sequence which encodes all or part of the structural gene and contains the promoter and ribosome binding site of a Bacillus coagulans amylase gene; within or immediately downstream of the structural gene is a site for insertion of heterologous DNA; when inserted, the heterologous DNA is in the same translational reading frame as the amylase gene; the heterologous DNA thus is under control of the B. coagulans amylase gene regulatory elements and expressed at high levels. A signal-encoding sequence may be inserted independent of, or along with, the heterologous DNA leading to authentic secretion of the heterologous protein.