Nancy Lim

US Patent:

7083916, Aug 1, 2006

Filed:

Mar 19, 2001

Appl. No.:

09/812074

Inventors:

Henry A. Lester - South Pasadena CA, US
Nathan Dascal - South Pasadena CA, US
Nancy F. Lim - Pasadena CA, US
Wolfgang Schreibmayer - South Pasadena CA, US
Norman Davidson - Sierra Madre CA, US

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

C12Q 1/68

US Classification:

435 6, 435 72, 435 691, 536 235

Abstract:

This invention provides isolated nucleic acid molecules which encode inward rectifier, G-protein activated, mammalian, potassium KGA channel. This invention also provides a nucleic acid molecule of at least 15 nucleotides capable of specifically hybridizing with the above nucleic acid molecule. This invention further provides a vector comprising the isolated nucleic acid molecules which encode inward rectifier, G-protein activated, mammalian, potassium KGA channel. This invention provides a host vector system for the production of a polypeptide having the biological activity of KGA channel which comprises the above vector in a suitable host. This invention also provides a method for isolating from a sample a nucleic acid molecule encoding an inward rectifier, G-protein activated, potassium channel in a sample which comprises: (a) isolating the nucleic acids from the sample; (b) contacting the isolated nucleic acids with the molecule of at least 15 nucleotides capable of specifically hybridizing with the above nucleic acid molecule which encode inward rectifier, G-protein activated, mammalian, potassium KGA channel under the conditions permitting complex formation between the nucleic acid molecule encoding an inward rectifier, G-protein activated, potassium channel and the nucleic acid molecule of at least 15 nucleotides capable of specifically hybridizing with the above nucleic acid molecule which encode inward rectifier, G-protein activated, mammalian, potassium KGA channel; (c) isolating the complex formed; and (d) separating the nucleic acid molecule encoding an inward rectifier, G-protein activated, potassium channel from the complex, thereby isolating the nucleic acid molecule encoding an inward rectifier, G-protein activated, potassium channel.

US Patent:

57285357, Mar 17, 1998

Filed:

Jun 7, 1995

Appl. No.:

8/486342

Inventors:

Henry A. Lester - South Pasadena CA
Nathan Dascal - South Pasadena CA
Nancy F. Lim - Pasadena CA
Wolfgang Schreibmayer - South Pasadena CA
Norman Davidson - Sierra Madre CA

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

G01N 3350
C07K 14705

US Classification:

435 72

Abstract:

This invention provides isolated nucleic acid molecules which encode inward rectifier, G-protein activated, mammalian, potassium KGA channel. This invention also provides a nucleic acid molecule of at least 15 nucleotides capable of specifically hybridizing with the above nucleic acid molecule. This invention further provides a vector comprising the isolated nucleic acid molecules which encode inward rectifier, G-protein activated, mammalian, potassium KGA channel. This invention provides a host vector system for the production of a polypeptide having the biological activity of KGA channel which comprises the above vector in a suitable host. This invention also provides a method for isolating from a sample a nucleic acid molecule encoding an inward rectifier, G-protein activated, potassium channel in a sample which comprises: (a) isolating the nucleic acids from the sample; (b) contacting the isolated nucleic acids with the molecule of at least 15 nucleotides capable of specifically hybridizing with the above nucleic acid molecule which encode inward rectifier, G-protein activated, mammalian, potassium KGA channel under the conditions permitting complex formation between the nucleic acid molecule encoding an inward rectifier, G-protein activated, potassium channel and the nucleic acid molecule of at least 15 nucleotides capable of specifically hybridizing with the above nucleic acid molecule which encode inward rectifier, G-protein activated, mammalian, potassium KGA channel; (c) isolating the complex formed; and (d) separating the nucleic acid molecule encoding an inward rectifier, G-protein activated, potassium channel from the complex, thereby isolating the nucleic acid molecule encoding an inward rectifier, G-protein activated, potassium channel.

US Patent:

57340218, Mar 31, 1998

Filed:

Jun 7, 1995

Appl. No.:

8/473092

Inventors:

Henry A. Lester - South Pasadena CA
Nathan Dascal - South Pasadena CA
Nancy F. Lim - Pasadena CA
Wolfgang Schreibmayer - South Pasadena CA
Norman Davidson - Sierra Madre CA

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

C07K 1435
C12N 1512

US Classification:

530350

Abstract:

This invention provides isolated nucleic acid molecules which encode inward rectifier, G-protein activated, mammalian, potassium KGA channel. This invention also provides a nucleic acid molecule of at least 15 nucleotides capable of specifically hybridizing with the above nucleic acid molecule. This invention further provides a vector comprising the isolated nucleic acid molecules which encode inward rectifier, G-protein activated, mammalian, potassium KGA channel. This invention provides a host vector system for the production of a polypeptide having the biological activity of KGA channel which comprises the above vector in a suitable host. This invention also provides a method for isolating from a sample a nucleic acid molecule encoding an inward rectifier, G-protein activated, potassium channel in a sample which comprises: (a) isolating the nucleic acids from the sample; (b) contacting the isolated nucleic acids with the molecule of at least 15 nucleotides capable of specifically hybridizing with the above nucleic acid molecule which encode inward rectifier, G-protein activated, mammalian, potassium KGA channel under the conditions permitting complex formation between the nucleic acid molecule encoding an inward rectifier, G-protein activated, potassium channel and the nucleic acid molecule of at least 15 nucleotides capable of specifically hybridizing with the above nucleic acid molecule which encode inward rectifier, G-protein activated, mammalian, potassium KGA channel; (c) isolating the complex formed; and (d) separating the nucleic acid molecule encoding an inward rectifier, G-protein activated, potassium channel from the complex, thereby isolating the nucleic acid molecule encoding an inward rectifier, G-protein activated, potassium channel.

US Patent:

6255459, Jul 3, 2001

Filed:

Mar 16, 1998

Appl. No.:

9/042494

Inventors:

Henry A. Lester - South Pasadena CA
Nathan Dascal - South Pasadena CA
Nancy F. Lim - Pasadena CA
Wolfgang Schreibmayer - South Pasadena CA
Norman Davidson - Sierra Madre CA

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

C07K 1628
C07K 14705
C12N 1512

US Classification:

5303882

Abstract:

The invention provides antibodies directed against a G protein-activated, inward rectifying KGA potassium channel polypeptide.

US Patent:

57472785, May 5, 1998

Filed:

May 21, 1993

Appl. No.:

8/066371

Inventors:

Henry A. Lester - South Pasadena CA
Nathan Dascal - South Pasadena CA
Nancy F. Lim - Pasadena CA
Wolfgang Schreibmayer - South Pasadena CA
Norman Davidson - Sierra Madre CA

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

C12N 1512
C07K 3817

US Classification:

435 691

Abstract:

Nucleic acids encoding mammalian "KGA" potassium channels are provided. Vectors and transformed host cells are also provided. The KGA channels exhibit inward rectification and are activated by a G-protein. The products of the invention are useful in screening assays to identify modulators of the KGA channel and in methods for identifying or preparing nucleic acids encoding the KGA channel or related potassium channels.