Nancy A Reichert

US Patent:

6357176, Mar 19, 2002

Filed:

Mar 19, 1997

Appl. No.:

08/825550

Inventors:

Brian S. Baldwin - Mississippi State MS
Marty J. Fuller - Mississippi State MS
Nancy A. Reichert - Mississippi State MS
Kevin L. Hensler - Mississippi State MS

Assignee:

Mississippi State University - Mississippi State MS

International Classification:

A01C 104

US Classification:

47 56

Abstract:

A soil- and grass seed-less sod precursor containing a non-woven bio-cellulosic fiber mat and grass sprigs. The sod precursor can be used to produce a soil-free sod which is useful for manufacturing athletic fields, golf courses and lawns.

US Patent:

6479287, Nov 12, 2002

Filed:

May 1, 2000

Appl. No.:

09/562193

Inventors:

Nancy A. Reichert - Starkville MS
Teong-Kwee Lim - Johor, MY
Margaret M. Young - Falmouth, JM

Assignee:

Mississippi State University - Mississippi State MS

International Classification:

C12N 502

US Classification:

435427, 435430, 4354301, 435431

Abstract:

Protocols for organogenic regeneration of cotton are provided, which makes the in vitro regeneration of mature fertile plants in a reduced amount of time possible. Seedlings are the basis for monocotyl or hypocotyl explants which are transferred from the germination medium to a shoot initiation medium which comprises AgNO. These explants, prior to shoot initiation, may be transformed with exogenous DNA, either through inoculation with an Agorbacterium agent such as A. tumefaciens, or through biolistic bombardment of the explants with microprojectiles having the exogenous DNA adsorbed onto their surface.

US Patent:

6570068, May 27, 2003

Filed:

Oct 30, 2000

Appl. No.:

09/698080

Inventors:

Margaret M. Young - Trelawny, JM
Nancy A. Reichert - Starkville MS

Assignee:

Mississippi State University - Mississippi State MS

International Classification:

A01H 100

US Classification:

800293, 800278, 800300, 8003001, 8003201, 435470, 435440, 435419, 435430, 435431

Abstract:

Maize tissue may be regenerated from nodal extracts prepared from germinated mature seeds and germinated embryos grown in a medium containing plant growth regulators. Nodal section explants are secured from seedlings approximately 3-10 days old, preferably from 3-7 days old. The explants are grown on an induction medium until adventitious shoot formation is observed. The shoots are separated and elongated on an MS-based medium, and then rooted. Fast genotype-independent regeneration is obtained, in 12-14 weeks. These explants, as well as zygotic embryos, may be transformed with exogenous DNA using a biolistic approach, where DNA precipitated onto tungsten microprojectiles is accelerated at a minimum of 650 psi towards the explants at a distance of at least 7. 5 cm. Improved frequency of transformation is obtained using microprojectiles which prior to DNA precipitation were frozen in glycerol, and suspending from a preparation of 2. 5 M CaCl. The combination of transformation process and regeneration can be used, independent of genotype, to provide new commercial crop organisms.

US Patent:

6703541, Mar 9, 2004

Filed:

Nov 21, 2000

Appl. No.:

09/716410

Inventors:

Linas S. Padegimas - Starkville MS
Nancy A. Reichert - Starkville MS

Assignee:

Mississippi State University - Mississippi State MS

International Classification:

C12N 1509

US Classification:

800279, 800278, 800287, 8003201, 800288, 435419, 435468, 4353201, 536 236, 536 232, 536 237, 536 241

Abstract:

The present invention provides a nematode inducible promoter sequence from maize inbred line Mp307, and DNA constructs and transcription cassettes comprising said promoter sequence, a gene encoding a toxin, and a termination sequence. Methods for inducing nematode resistance in plants and transgenic plants resistant to nematode infection are also provided.

US Patent:

61405558, Oct 31, 2000

Filed:

Jun 5, 1998

Appl. No.:

9/092180

Inventors:

Nancy A. Reichert - Starkville MS
Vanishree Rudraswamy - Starkville MS

Assignee:

Mississippi State University - Mississippi State MS

International Classification:

A01H 400
A01H 100
C12N 504
C12N 1582
C12N 1587

US Classification:

800293

Abstract:

Maize tissue may be regenerated from nodal extracts prepared from germinated mature seeds and germinated embryos. Nodal section explants are secured from seedlings in 3-5 days. The explants are grown on an induction medium until adventitious shoot formation is observed. The shoots are separated and elongated on an MS-based medium, and then rooted. Fast genotype-independent regeneration is obtained, in 12-14 weeks. These explants, as well as zygotic embryos, may be transformed with exogenous DNA using a biolistic approach, where DNA precipitated onto tungsten microprojectiles is accelerated as 650 psi towards the explants at a distance of at least 7. 5 microns. Improved frequency of transformation is obtained using microprojectiles which prior to DNA precipitation were frozen in glycerol, and suspending from a preparation of 2. 5 M CaCl. sub. 2. The combination of transformation process and regeneration can be used, independent of genotype, to provide new commercial crop organisms.

US Patent:

61538132, Nov 28, 2000

Filed:

Dec 11, 1998

Appl. No.:

9/209466

Inventors:

Nancy A. Reichert - Mississippi State MS
Vanishree Rudraswamy - Mississippi State MS
Liza Ming-Ju Chen - Starkville MS

Assignee:

Mississippi State University - Mississippi State MS

International Classification:

A07H 100
C12N 1529
C12N 1582
C12N 1500
A01H 106

US Classification:

800293

Abstract:

A method for transforming rice plants to express heterologous DNA which involves the biolistic bombardment of mature rice seeds (embryo bearing) and results in expression of the heterologous DNA coated on the biolistic particle in the post-bombardment embryo. The heterologous DNA may be introduced as a plastid, as opposed to bare DNA. The transformed rice embryo is regenerated into a rice plantlet which can be transferred from greenhouse to field.

US Patent:

59688300, Oct 19, 1999

Filed:

Mar 28, 1997

Appl. No.:

8/825469

Inventors:

Yinghui Dan - Mississippi State MS
Nancy A. Reichert - Mississippi State MS

Assignee:

Mississippi State University - Mississippi State MS

International Classification:

C12N 510
C12N 1582
A01H 500

US Classification:

435469

Abstract:

A method of transforming and regenerating soybean plants relies on selection of hypocotyl explants as the target material. Hypocotyl explants can be transformed either by microparticle bombardment with DNA-coated microparticles of inert metals, or by co-culturing with an Agrobacterium strain. The transformed explants can be successfully regenerated, using a protocol including culturing on a shoot induction medium, followed by transfer to a shoot elongation medium to form rooted plantlets, which are transplanted to soil.

US Patent:

59982071, Dec 7, 1999

Filed:

Jun 12, 1998

Appl. No.:

9/096360

Inventors:

Nancy A. Reichert - Starkville MS
Teong-Kwee Lim - Johor, MY
Margaret M. Young - Starkville MS

Assignee:

Mississippi State University - Mississippi State MS

International Classification:

A01H 400
A01H 100
C12N 1582
C12N 1584

US Classification:

435430

Abstract:

Protocols for ogranogenic regeneration of cotton and kenaf are provided, which makes the in vitro regeneration of mature fertile plants in a reduced amount of time possible. Seedlings are the basis for monocotyl or hypocotyl explants which are transferred from the germination medium to a shoot initiation medium which comprises AgNO. sub. 3. These explants, prior to shoot initiation, may be transformed with exogenous DNA either through inoculation with a Agrobacterium agent such as A. tumefaciens, or through biolistic bombardment of the explants with microprojectiles having the exogenous DNA adsorbed onto their surface.