Narasimha Chary Samboju

US Patent:

8581036, Nov 12, 2013

Filed:

Mar 8, 2011

Appl. No.:

13/042565

Inventors:

Narasimha C. Samboju - Carmel IN, US
Jayakumar P. Samuel - Carmel IN, US
Gaofeng Lin - Zionsville IN, US
Steven R. Webb - Westfield IN, US
Frank Burroughs - Noblesville IN, US

Assignee:

Dow AgroSciences, LLC. - Indianapolis IN

International Classification:

C12N 15/82
C12N 15/87
C12N 5/00
C12N 5/02
C12N 15/29

US Classification:

800278, 435470, 435425, 4354301, 800293, 536 236

Abstract:

A method of introducing a molecule of interest into a plant cell having a cell wall includes interacting a gamma-zein peptide with a molecule of interest to form a gamma-zein linked structure. The gamma-zein linked structure is then placed in contact with the plant cell having a cell wall, and allowing uptake of the gamma-zein linked structure into the plant cell. Alternatively, a gene of interest can be expressed in a plant cell having an intact cell wall by interacting a gamma-zein peptide with the gene of interest to form a gamma-zein linked gene structure, allowing uptake of the gamma-zein linked gene structure into the plant cell, and expressing the gene of interest in the plant cell and its progeny.

US Patent:

8609420, Dec 17, 2013

Filed:

Mar 22, 2012

Appl. No.:

13/427750

Inventors:

Jayakumar P. Samuel - Carmel IN, US
Narasimha C. Samboju - Carmel IN, US
Kerrm Y. Yau - Carmel IN, US
Gaofeng Lin - Zionsville IN, US
Steven R. Webb - Westfield IN, US
Frank Burroughs - Noblesville IN, US

Assignee:

Dow AgroSciences, LLC. - Indianapolis IN

International Classification:

C07K 14/415
C12N 15/87
C12N 15/74
A01H 5/10
B82Y 10/00
B82Y 30/00
B82Y 5/00

US Classification:

435468, 435470, 435476, 800293, 8003201, 977728, 977774, 977704, 977705

Abstract:

Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using a QD-peptide conjugate having a quantum dot (QD) with one or more cell penetrating peptides (CPPs). Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

US Patent:

20100311168, Dec 9, 2010

Filed:

Apr 7, 2010

Appl. No.:

12/755447

Inventors:

Jayakumar P. Samuel - Carmel IN, US
Joseph F. Petolino - Zionsville IN, US
Narasimha C. Samboju - Carmel IN, US
Steven R. Webb - Westfield IN, US
Kerrm Y. Yau - Carmel IN, US

Assignee:

DOW AGROSCIENCES LLC - Indianapolis IN

International Classification:

C12N 5/02

US Classification:

435425, 435420, 435430, 435427, 435426, 435424

Abstract:

Provided are methods for introducing a sequence specific nuclease into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

US Patent:

20110093982, Apr 21, 2011

Filed:

Oct 8, 2010

Appl. No.:

12/901312

Inventors:

Jayakumar P. Samuel - Carmel IN, US
Narasimha C. Samboju - Carmel IN, US
Kerrm Y. Yau - Carmel IN, US
Steven R. Webb - Westfield IN, US
Frank Burroughs - Noblesville IN, US

Assignee:

DOW AGROSCIENCES LLC - Indianapolis IN

International Classification:

C12N 15/88
C12N 5/04
C12N 15/82

US Classification:

800278, 435410, 435414, 435412, 435415, 435430, 435468

Abstract:

Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using dendrimers, and optionally one or more CPPs. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

US Patent:

20120023619, Jan 26, 2012

Filed:

Jul 7, 2011

Appl. No.:

13/178199

Inventors:

Narasimha C. Samboju - Carmel IN, US
Kerrm Y. Yau - Carmel IN, US
Frank Burroughs - Noblesville IN, US
Jayakumar P. Samuel - Carmel IN, US
Steven R. Webb - Westfield IN, US

Assignee:

DOW AGROSCIENCES LLC - Indianapolis IN

International Classification:

C12N 15/82
C12N 5/04
B82Y 5/00

US Classification:

800279, 435470, 435410, 435412, 435414, 800278, 435415, 977774, 977904

Abstract:

Methods for introducing a linear nucleic acid molecule of interest into a cell comprising a cell wall include use of nanoparticles coated with polyethylene glycol. In some embodiments, the cell comprising a cell wall is a plant cell. Methods include genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with linear nucleic acid molecules.

US Patent:

20120023620, Jan 26, 2012

Filed:

Jul 7, 2011

Appl. No.:

13/178235

Inventors:

Kerrm Y. Yau - Carmel IN, US
Jayakumar P. Samuel - Carmel IN, US
Frank Burroughs - Noblesville IN, US
Narasimha C. Samboju - Carmel IN, US
Steven R. Webb - Westfield IN, US

Assignee:

DOW AGROSCIENCES LLC - Indianapolis IN

International Classification:

C12N 15/82
C12N 5/04
B82Y 5/00

US Classification:

800279, 435470, 435410, 435412, 435414, 435415, 800278, 977774, 977902

Abstract:

Methods for introducing a functionalized linear nucleic acid cassette molecule of interest into a plant cell comprising a cell wall include use of nanoparticles. In some embodiments, the cell comprising a cell wall is a cultured plant cell. Methods include genetically or otherwise modifying plant cells and for treating or preventing disease in any plant, especially crop plants. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with functionalized linear nucleic acid cassette molecules.

US Patent:

20120034697, Feb 9, 2012

Filed:

Aug 10, 2011

Appl. No.:

13/206637

Inventors:

P. Samuel Jayakumar - Carmel IN, US
Jeffrey Beringer - Carmel IN, US
Paul R. Schmitzer - Indianapolis IN, US
Frank Burroughs - Noblesville IN, US
Robbi J. Garisson - Fillmore IN, US
William M. Ainley - Carmel IN, US
Narasimha C. Samboju - Carmel IN, US

Assignee:

Dow AgroSciences LLC - Indianapolis IN

International Classification:

C12N 5/04

US Classification:

435412, 435410, 435414

Abstract:

The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.

US Patent:

20170022521, Jan 26, 2017

Filed:

Sep 22, 2015

Appl. No.:

14/861864

Inventors:

- Indianapolis IN, US
Joseph F. Petolino - Zionsville IN, US
Narasimha Chary Samboju - Carmel IN, US
Steven R. Webb - Westfield IN, US
Kerrm Y. Yau - Carmel IN, US

International Classification:

C12N 15/90
C12N 9/96
C12N 5/04
C12N 9/22

Abstract:

Provided are methods for introducing a sequence-specific nuclease into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.