Nathan A Greene

US Patent:

7645608, Jan 12, 2010

Filed:

Aug 15, 2005

Appl. No.:

11/203929

Inventors:

Nathan Greene - Thousand Oaks CA, US

Assignee:

PML Microbiologicals, Inc. - Wilsonville OR

International Classification:

C12M 1/26
C12M 1/36
C12M 3/00

US Classification:

4352863, 4353091, 4353092, 435810

Abstract:

A device for storing, rehydrating, transferring, and streaking a stored freeze dried specimen comprises a three piece construction including a collection vial used to store a freeze dried specimen, a capsule including a reservoir with hydrating fluid retained by a frangible membrane, and an adapter fluidly linking the capsule to the collection vial. The adapter is configured to fluidly link to the capsule to the collection vial via leak-proof couplings to create a closed system of specimen, hydrating fluid, and a small quantity of trapped air. The fluid communication between the vial and the capsule is accomplished through the adapter via an internal lumen longitudinally traversing the adapter. The adapter and capsule combination can also serve as a transfer pipette once the hydration operation is complete to withdraw the specimen from the collection vial and transfer the specimen to a petri dish or other culture medium. The adapter and capsule combination is quickly detachable from the collection vial to allow an inoculating tip exposed to the specimen to be transferred conveniently to a media for culturing with minimal exposure.