Nathan C Moore

US Patent:

20230080444, Mar 16, 2023

Filed:

Sep 16, 2022

Appl. No.:

17/946862

Inventors:

- Cambridge MA, US
Bryan D. Teece - Boston MA, US
James Gillett Truslow - Boston MA, US
Nathan Francis Moore - Canton MA, US
Jeffrey T. Borenstein - West Roxbury MA, US
Vishal Tandon - Somerville MA, US

International Classification:

C12M 1/02
C12M 1/12
C12N 15/87
C12M 1/00
C12N 15/86
C12M 3/06
C12M 1/40
C12M 1/34

Abstract:

A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.

US Patent:

20230063282, Mar 2, 2023

Filed:

Sep 16, 2022

Appl. No.:

17/946948

Inventors:

- Cambridge MA, US
Bryan D. Teece - Boston MA, US
James Gillett Truslow - Boston MA, US
Nathan Francis Moore - Canton MA, US
Jeffrey T. Borenstein - Holliston MA, US
Vishal Tandon - Somerville MA, US

Assignee:

THE CHARLES STARK DRAPER LABORATORY, INC. - Cambridge MA

International Classification:

C12M 1/02
C12M 1/12
C12N 15/87
C12M 1/00
C12N 15/86
C12M 3/06
C12M 1/40
C12M 1/34

Abstract:

A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.

US Patent:

20190307946, Oct 10, 2019

Filed:

Jun 25, 2019

Appl. No.:

16/452191

Inventors:

- Cambridge MA, US
Kenneth T. Kotz - Newton MA, US
Nathan Francis Moore - Canton MA, US

International Classification:

A61M 1/36
B01L 3/00
B03B 1/04

Abstract:

A method for separating cells in a biofluid includes pretreating the biofluid by introducing an additive comprising a cell activator, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel to accumulate target cells in a primary stream and non-target cells in a secondary stream. A system for microfluidic cell separation capable of separating target cells from non-target cells in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive comprising a cell activator, and at least one acoustic transducer coupled to the microfluidic separation channel.

US Patent:

20190085280, Mar 21, 2019

Filed:

Sep 20, 2018

Appl. No.:

16/137478

Inventors:

- Cambridge MA, US
Bryan D. Teece - Boston MA, US
James Gillett Truslow - Boston MA, US
Nathan Francis Moore - Canton MA, US

International Classification:

C12M 1/02
C12M 1/12
C12M 1/00
C12N 15/87

Abstract:

A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.

US Patent:

20180280971, Oct 4, 2018

Filed:

Mar 30, 2018

Appl. No.:

15/942052

Inventors:

- Cambridge MA, US
Alla A. Gimbel - Medford MA, US
Jose A. Santos - Westwood MA, US
Daniel T. Doty - Arlington MA, US
Nathan F. Moore - Providence RI, US
Louis B. Kratchman - Quincy MA, US
James Truslow - Boston MA, US

International Classification:

B01L 3/00
C12Q 1/24
G01N 33/50

Abstract:

Systems and methods for conducting assays on tissue fragment samples including providing a suspension maintaining pump, and a plurality of fluid reservoirs, wherein the fluid reservoirs are configured to hold a volume of fluid. The fluid reservoirs are fluidically coupled to a microfluidic assay chip, wherein the microfluidic assay chip includes a plurality of parallel assay channels, a first inlet port for introduction of a tissue fragment sample into the microfluidic assay ship, and a second inlet port coupled to the fluid reservoir. Each channel of the microfluidic assay chip also includes a retention barrier configured to trap the tissue fragment sample such that the fluid perfuses through the tissue sample, as well as an outlet port fluidically coupled to a waste receptacle.