Nhung T Nguyen

US Patent:

20200095576, Mar 26, 2020

Filed:

Sep 24, 2019

Appl. No.:

16/581587

Inventors:

- College Station TX, US
Yun Huang - Houston TX, US
Nhung T. Nguyen - Houston TX, US
Lian He - Houston TX, US

International Classification:

C12N 15/11
C12N 9/22
C12N 15/90

Abstract:

In an embodiment, a device for modulating intracellular gene expression, the device having a calcium actuator component and a transcription reprogramming component. In another embodiment, a method for modulating intracellular gene expression, where the method includes inducing a system having a calcium actuator component and a transcription reprogramming component with at least one of light and a chemical, causing an increase in Ca, and translocating the transcription reprogramming component from cytosol to the nucleus. In a further embodiment, a method for modulating gene intracellular expression, where the method includes inducing a system having an NIR-stimulable optogenetic platform with at least one of light and a chemical, where the NIR-stimulable optogenetic platform facilitates Ca release and the NIR-stimulable optogenetic platform is LOV2-SOAR, causing an increase in Ca, and translocating a calcium-responsive dCas9 fusion construct from cytosol to the nucleus, where the calcium-responsive dCas9 fusion construct is NFAT-dCas9-VP64.

Address:

4514 Evergreen Mdw Ct, Katy, TX 77449

VIN:

5FNRL38297B034519

Make:

HONDA

Model:

ODYSSEY 4DR WGN LX

Year:

2007