Nicole M Perry

US Patent:

20140286932, Sep 25, 2014

Filed:

Mar 21, 2014

Appl. No.:

14/221755

Inventors:

Konstantinos Konstantopoulos - Ellicott City MD, US
Marey Shriver - Nottingham MD, US
Nicole Perry - Baltimore MD, US

Assignee:

JOHNS HOPKINS UNIVERSITY - Baltimore MD
UNIVERSITY OF MARYLAND, BALTIMORE - Baltimore MD

International Classification:

G01N 33/574
C07K 16/40
A61N 5/06
C12Q 1/68
A61N 5/10
A61K 38/17
A61K 45/06

US Classification:

4241301, 514 44 R, 5303879, 424649, 435 71, 435 611, 435 612, 435 74, 435 792, 435375, 600 1, 600 2

Abstract:

Provided herein are methods and kits for evaluating potential for invasiveness, metastasis, or recurrence of an epithelial cell cancer. In the methods the expression profile of giant obscurins is detected in a tissue sample of tumor cells or suspected tumor cells and assessed for giant obscuring expression level and distribution therein. Decreased levels or altered distribution of giant obscurins in the cells compared to a control non-invasive standard or to a sample taken at a different point in time is indicative of increased potential of at least one of the invasiveness, metastasis, or recurrence of the epithelial cell cancer. The kit comprises a detection reagent suitable for detecting the presence and distribution of giant obscurins or an amount of the gene product(s) encoding giant obscurins in cells of a tissue sample and instructions for using the detection reagent.