Pablo Sobrado from 410 Heartwood Xing, Blacksburg, VA 24060, age 50

Resumes

Associate Professor At Virginia Tech

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Position:

Associate Professor, Department of Biochemistry at Virginia Tech

Location:

Roanoke, Virginia Area

Industry:

Higher Education

Work:

Virginia Tech - Virginia Tech, Blacksburg, VA 24061 since Aug 2007
Associate Professor, Department of Biochemistry

Education:

University of Wisconsin-Madison 2004 - 2007
Reseach Associate, Enzymology Universidad de Chile 2003 - 2004
Postdoctoral fellow, Protein kinases Texas A&M University 1997 - 2003
Doctor of Philosophy (PhD), Biochemistry, Biophysics and Molecular Biology Merrimack College 1993 - 1997
Bachelor of Arts (BA), Biology/Biological Sciences, General

Pablo Sobrado

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Us Patents

Expression Systems For Mammalian And Mycobacterial Desaturases
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US Patent:

20080182249, Jul 31, 2008
Filed:

Sep 4, 2007
Appl. No.:

11/899081
Inventors:

Brian G. Fox - Madison WI, US
Pablo Sobrado - Blacksburg VA, US
Yong Chang - Madison WI, US
International Classification:

C12Q 1/68
C12N 15/63
US Classification:

435 6, 4353201
Abstract:

Expression system for components of a desaturase complex is provided. The system includes expression of a desaturase and an oxidoreductase. The system may be used for expression of mycobacterial desaturases or for expression of mammalian desaturases. The system may further include cell-free expression of other components of the desaturase complex. The expression system may include expression of stearoyl-CoA. The expression system may further include expression of cytochrome b5. The expression system may also include expression of cytochrome b5 reductase. The expression system may also include expression of Rv3230c. In addition, methods for assaying the activity of a stearoyl-CoA desaturase in vitro are provided.

Expression Systems For Functional Membrane Polypeptides
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US Patent:

20100330649, Dec 30, 2010
Filed:

Nov 10, 2008
Appl. No.:

12/267825
Inventors:

Brian G. Fox - Madison WI, US
Pablo Sobrado - Blacksburg VA, US
International Classification:

C12N 9/48
C12N 15/63
C07K 14/00
US Classification:

435212, 4353201, 530324
Abstract:

Expression systems and methods for the expression of functional membrane polypeptides such as human cytochrome b5 are provided. The systems include recombinant expression vectors capable of expressing soluble fusion proteins that include a solubilizing agent, a linker, and a membrane polypeptide, as well as one or more cleavers, e.g. proteases, capable of cleaving the linker to release the membrane polypeptide. When the fusion protein is expressed, the linker is cleaved by the cleaver to allow association of the membrane polypeptide with a membrane.