Pamela S Fink

US Patent:

7718791, May 18, 2010

Filed:

Mar 31, 2006

Appl. No.:

11/396108

Inventors:

Brian Bahler - Apex NC, US
Jason Arnold Lotvin - Union NJ, US
Mark Edward Ruppen - Garnerville NY, US
Pamela Sue Fink Charbonneau - New City NY, US

Assignee:

Wyeth LLC - Madison NJ

International Classification:

C07H 1/06
C07H 1/00
C12P 19/04

US Classification:

536127, 5361231, 435101

Abstract:

A process for reducing the protein content and preserving the capsular polysaccharide content in a complex cellular lysate broth prior to purification is described. Utilizing pH reduction after cellular lysis has resulted in a purified polysaccharide that consistently meets the protein specification, and higher recovery yields of polysaccharide during the purification process.

US Patent:

20080286838, Nov 20, 2008

Filed:

Mar 20, 2008

Appl. No.:

12/052525

Inventors:

Yonghui Yuan - Tappan NY, US
Mark Ruppen - Garnerville NY, US
Wei-Qiang Sun - Morristown NJ, US
Ling Chu - Suffern NY, US
John Simpson - Upper Nyack NY, US
James Patch - Cornwall on Hudson NY, US
Justin Keith Moran - Valley Cottage NY, US
Pamela Fink - New City NY, US

Assignee:

Wyeth - Madison NJ

International Classification:

C12P 19/04

US Classification:

435101

Abstract:

A shortened process for producing a solution containing substantially purified capsular polysaccharides from a cellular lysate broth is described. Ultrafiltering and diafiltering a clarified lysate followed by pH adjustment to less than 4.5, preferably about 3.5, precipitated at least 98% of the protein in the solution without seriously affecting polysaccharide yield. Furthermore, following ultrafiltration and diafiltration and acidification to a pH of less than 4.5, filtration using activated carbon precipitated at least 90% of remaining protein without seriously affecting polysaccharide yield. Exemplary, non-limiting serotypes that can be purified using the shortened process of the invention are 1, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, and 23F. In one embodiment, the cells are lysed using deoxycholate sodium (DOC), while in another embodiment the lytic agent is a non-animal derived lytic agent such as N-lauryl sarcosine sodium (NLS).

US Patent:

20100028952, Feb 4, 2010

Filed:

Jun 21, 2007

Appl. No.:

12/305428

Inventors:

Eugene Joseph Vidunas - Middletown NY, US
Gow-Jen Tsai - Airmont NY, US
Ping Cai - New City NY, US
Justin Moran - Valley Cottage NY, US
Pamela Sue Fink - New City NY, US
Lourdes Jeanette Gordon - White Plains NY, US

Assignee:

WYETH - Madison NJ

International Classification:

C12P 21/00
C12N 1/20
C07K 1/00

US Classification:

435 712, 4352526, 530416

Abstract:

The present invention provides methods for production and purification of active chromoproteins produced by sp. 21G792. The chromoproteins are useful for developing pharmaceutical compositions and treating diseases such as cancer or bacterial infections.