Pamela S Olson

US Patent:

56291728, May 13, 1997

Filed:

Aug 2, 1994

Appl. No.:

8/284784

Inventors:

Desmond Mascarenhas - Sunnyvale CA
Yang Zhang - Sunnyvale CA
Pamela S. Olson - Cupertino CA
David R. Olsen - Menlo Park CA
Pedro A. Cohen - San Francisco CA

Assignee:

Celtrix Pharmaceuticals, Inc. - Santa Clara CA

International Classification:

C12N 119
C12N 1562
C07K 1400

US Classification:

435 647

Abstract:

The invention is directed to the use of a fusion partner that does not contain a leader sequence, as a means to increase the solubility and activity of recombinant polypeptides by facilitating the expression of fusion proteins, which are then transported out of the cytoplasm. The invention includes a nucleic acid encoding a fusion polypeptide comprising a mature interleukin-1-like polypeptide or a leader-deleted-translocating polypeptide, and a polypeptide of interest; as well as host cells comprising such nucleic acids, and fusion proteins so encoded. The invention also encompasses methods of using such nucleic acids to produce recombinant fusion polypeptides, mature polypeptides of interest, and purified compositions thereof.

US Patent:

59142545, Jun 22, 1999

Filed:

May 12, 1997

Appl. No.:

8/854811

Inventors:

Desmond Mascarenhas - Los Altos Hills CA
Yang Zhang - Sunnyvale CA
Pamela S. Olson - Cupertino CA
David R. Olsen - Menlo Park CA
Pedro A. Cohen - San Francisco CA
Kimyen B. Nguyen - Union City CA

Assignee:

Celtrix Pharmaceuticals, Inc. - Santa Clara CA

International Classification:

C07K 1900
C12N 1562

US Classification:

435 697

Abstract:

The invention is directed to the use of a fusion partner that does not contain a leader sequence, as a means to increase the solubility and activity of recombinant polypeptides by facilitating the expression of fusion proteins, which are then transported out of the cytoplasm. The invention includes a nucleic acid encoding a fusion polypeptide comprising a mature interleukin-1-like polypeptide or a leader-deleted-translocating polypeptide, and a polypeptide of interest; as well as host cells comprising such nucleic acids, and fusion proteins so encoded. The invention also encompasses methods of using such nucleic acids to produce recombinant fusion polypeptides, mature polypeptides of interest, and purified compositions thereof.

US Patent:

58612734, Jan 19, 1999

Filed:

Jun 7, 1995

Appl. No.:

8/482182

Inventors:

Pamela S. Olson - Cupertino CA
Desmond Mascarenhas - Los Altos Hills CA

Assignee:

Celtrix Phamraceuticals, Inc. - Santa Clara CA

International Classification:

C12P 2102
C12N 121
C12N 1564
C12N 1570

US Classification:

435 691

Abstract:

The present invention provides compositions and methods for producing a heterologous protein of interest by inserting a copy of a gene encoding the heterologous protein of interest into the chromosome of a host cell, such as E. coli. A chromosomal transfer DNA (a circular, non-self-replicating DNA) is used to integrate the gene encoding the heterologous protein of interest into the host cell chromosome. The chromosomal transfer DNA comprises at least one selectable marker and may optionally include repeated DNA sequences flanking the selectable marker, facilitating chromosomal amplification of the integrated DNA. The gene encoding the protein of interest may be expressed after integration into the chromosome of the host cell; selection for chromosomal amplification may be performed prior to expression of the gene.